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I. j. Aqua. Anim. Health 2017, 3(2): 74-85 Back to browse issues page
Evaluation of silver nanoparticles toxicity in Daphnia magna: Comparison of chemical and green biosynthetic productions
Z Tulaby Dezfuly, M Alishahi , A Aramoon, S Mashjoor
Abstract:   (1536 Views)
Recently nanoparticles, particularly silver nanoparticles, are broadly used in industry, hence the contamination of the environment with AgNPs has caused considerable concern. In this study, the toxicity of biosynthetic nanosilver produced by two macroalgae: Sargassum boveanum and Ulva flexuosa extracts were compared with chemical nanosilver in Daphnia magna. Size and quality of nanoparticles evaluated by TEM, FT-IR spectrum, and Particle size analyzer. The acute toxicity test was evaluated following the OECD and Test guideline No: 211. D. magna were reproduced using parthenogenesis from a single individual according to OECD guideline. Then Daphnia exposed to eight serial dilutions of each nanosilver in triplicates for 48 hours. The mortality rate after 12h, 24h, 36h, and 48h were recorded and analyzed using probit software. Results showed that all nanosilver (regardless of their synthesis origin) were toxic in Daphnia and acute toxicity of this nanosilver was different (p<0.05).  The 48h LC50 of SPN, UPN, and CPN in Daphnia were 1.03, 3.24 and 0.03 mg L-1 respectively. The mortality rate in D. magna enhanced in all tested groups, along with increasing nanosilver concentration and exposure time duration. Highest toxicity belongs to chemical nanosilver (LC50 = 0.03 mg L-1), which was 30 and 100 times more toxic than SP (LC50 = 1.03) and UP (LC50 = 3.24 mg L-1) respectively. According to the high toxicity of chemosynthetic nanosilver compare to biosynthetic ones, biosynthetic nanoparticles are highly recommendable and environmentally friendly alternative to chemical oriented nanoparticles.

Keywords: Nanosilver, Daphnia magna, Sargassum boveanum, Ulva flexuosa, Biosynthetic
Full-Text [PDF 943 kb]   (627 Downloads)    
Type of Study: Research | Subject: Toxicology and polution
Received: 2017/06/7 | Accepted: 2017/10/14 | Published: 2017/11/4
1. Asghari S., Johari S.A., Lee J.H., Kim Y.S., Jeon Y.B., Choi H.J., Moon M.C. and Yu I.J. (2012) Toxicity of various silver nanoparticles compared to silver ions in Daphnia magna. Journal of Nanobiotechnology 2, 10 (1):14. [DOI:10.1186/1477-3155-10-14]
2. Baird D.J., Barber I., Bradley M., Calow P. and Soares A.V.M. (1989) The Daphnia bioassay: a critique., Hydrobiologia 188/189, 403-406. [DOI:10.1007/BF00027806]
3. Benn T.M. and Westerhoff P. (2008) Nanoparticle silver released into water from commercially available sock fabrics. Environmental Science and Technology 42, 4133–4139. [DOI:10.1021/es7032718]
4. Bianchini A. and Wood C. (2003) Mechanism of acute silver toxicity in Daphnia magna. Environmental Toxicology and Chemistry 22, 1361–1367. [DOI:10.1002/etc.5620220624]
5. Burrows E.M. (1991) Seaweeds of the British Isles. Volume 2 Chlorophyta. Natural History Museum Publications. London ISBN 0-565-00981-8.
6. Fung M.C. and Bowen D. (1996) Silver products for medical indications: risk-benefit assessment. The Journal of Clinical Toxicology 34, 119–126. [DOI:10.3109/15563659609020246]
7. Gatoo M.A., Naseem S., Arfat M.Y., Dar A.M., Qasim K. and Zubair S. (2014) Physicochemical properties of nanomaterials: implication in associated toxic manifestations. BioMed Research International 6, 1-8. [DOI:10.1155/2014/498420]
8. Griffitt R.J., Luo J., Gao J., Bonzongo J.C., and Barber D.S. (2008) Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environmental Toxicology and Chemistry 9, 1972–1978. [DOI:10.1897/08-002.1]
9. Hardy F.G. and Guiry M.D. (2003) A Check-list and Atlas of the Seaweeds of Britain and Ireland.The British Phycological Society. ISBN 0 9527115 16. p 29.
10. Hedayati A., Kolangi H., Jahanbakhshi A., and Shaluei F. (2012) Evaluation of silver nanoparticles ecotoxicity in silver carp (Hypophthalmicthys molitrix) and goldfish (Carassius auratus). Bulgarian Journal of Veterinary Medicine 15, 172−177.
11. Hogstrand C. and Wood C.M. (1998) Towards a better understanding of the bioavailability, physiology and toxicity of silver to fish: Implications for water quality criteria. Environmental Toxicology and Chemistry 17, 572–578. [DOI:10.1002/etc.5620170405]
12. Kahru A. and Dubourguier H.C. (2010) From ecotoxicology to nanoecotoxicology. Toxicology 269, 105–119. [DOI:10.1016/j.tox.2009.08.016]
13. Kumar P., Senthamilselvi S., Lakshmi prabha A., Selvaraj S., Macklin Rani L., Suganthi P., Sarojini Devi B. and Govindaraju M. (2012) Antibacterial activity and In-vitro cytotoxicity assay against brine shrimp using silver nanoparticles synthesized from Sargassum iliifolium. Digest Journal of Nanomaterials and Biostructures 7, 1447–1455.
14. Kumar P., Senthamilselvi S., Lakshmi Prabha A., Premkumar K., Muthukumaran R., Visvanathan P., Ganeshkumar R.S. and Govindaraju M. (2012a) Efficacy of biosynthesized silver nanoparticles using Acanthophora spicifera to encumber biofilm formation. Digest Journal of Nanomaterials and Biostructures 7, 511–522.
15. Kumar p., Senthamil Selvi S. and Govindaraju M. (2012) Seaweed-mediated biosynthesis of silver nanoparticles using Gracilaria corticata for its antifungal activity against Candida spp. Applied Nanoscience 3(6), 495-500.
16. Lapresta-Ferna´ndez A., Ferna´ndez A. and Blasco J. (2012) Nanoecotoxicity effects of engineered silver and gold nanoparticles in aquatic organisms. Trends in Analytical Chemistry 32, 40-59. [DOI:10.1016/j.trac.2011.09.007]
17. Lovri´c J., Bazzi H.S., Cuie Y., Fortin G.R.A., Winnik F.M. and Maysinger D. (2005) Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots. Journal of Molecular Medicine 83, 377–385. [DOI:10.1007/s00109-004-0629-x]
18. Luoma S.N. (2008) Silver nanotechnologies and the environment: Old problems or new challenges. PEN 15. Project on Emerging Nanotechnologies, Woodrow Wilson International Center for Scholar, Washington, DC, p 65.
19. Mueller N.C. and Nowack B. (2008) Exposure modeling of engineered nanoparticles in the environment. Environmental Science and Technology 42, 4447–4453. [DOI:10.1021/es7029637]
20. Nunes B.S., Carvalho F.D., Guilhermino L.M., Stappen G.V. (2006) Use of the genus Artemia in ecotoxicity testing. Environmental Pollution 144, 453-462. [DOI:10.1016/j.envpol.2005.12.037]
21. OECD (Organisation for Economic Co-operation and Development) 202 (2004) OECD guidelines for the testing of chemicals. Daphnia sp., acute immobilization test. Paris, France.
22. OECD (Organisation for Economic Co-operation and Development) 211 (2008) OECD guidelines for the testing of chemicals. Daphnia sp., reproduction test. Paris, France.
23. Parikh R.Y., Singh S., Prasad B.L.V., Patole M.S., Sastry M. and Shouche Y.S. (2008) Extracellular synthesis of crystalline silver nanoparticles and molecular evidence of silver resistance from Morganella sp.: towards understanding biochemical synthesis mechanism. Journal of chemical biology 9, 1415–1422.
24. Ribeiro, F., Gallego-Urrea J.A., Jurkschat K., Crossley A., Hassellöv M., Taylor C., Soares A.M.V.M. and Loureiro S. (2014) Silver nanoparticles and silver nitrate induce high toxicity to Pseudokirchneriella subcapitata, Daphnia magna and Danio rerio. Science of the Total Environment 466–467, 232–241. [DOI:10.1016/j.scitotenv.2013.06.101]
25. Sam N., Palanichamy S., Chellammal S., Kalaiselvi P. and Subramanian G. (2015) Antifouling effects of silver nanoparticles synthesized from tropical seaweeds. International Journal of Current Microbiology and Applied Sciences 4, 1029-1042.
26. Shen M.H., Zhou X.X., Yang X.Y., Chao J.B., Liu R., and Liu J.F. (2015) Exposure medium: key in identifying free Ag1 as the exclusive species of silver nanoparticles with acute toxicity to Daphnia magna. Scientific Reports 5, 1-8. [DOI:10.1038/srep09674]
27. Silva P.C., Bassoon Ph.W. and Moe R.L. (1996) Catalogue of the Benthic Marine Algae of the Indian Ocean, University of California 79, 661.
28. Sotiriou G.A. and Pratsinis S.E. (2010) Antibacterial activity of nanosilver ions and particles. Environmental Science and Technology 44, 5649–5654. [DOI:10.1021/es101072s]
29. Vijayan R.S., Santhiyagu P., Singamuthu M., Kumari Ahila N., Jayaraman R., and Ethiraj K. (2014) Synthesis and Characterization of Silver and Gold Nanoparticles Using Aqueous Extract of Seaweed, Turbinaria conoides, and Their Antimicrofouling Activity. The Scientific World Journal 2014, 1-11. [DOI:10.1155/2014/938272]
30. Wang Z., Chen J., Li X., Shao J. and Peijnenburg W.J. (2012) Aquatic toxicity of nanosilver colloids to different trophic organisms: Contributions of particles and free silver ion. Environmental Toxicology and Chemistry 31, 2408–2413. [DOI:10.1002/etc.1964]
31. Zhao C.M. and Wang W.X. (2012) Importance of surface coatings and soluble silver in silver nanoparticles toxicity to Daphnia magna. Nanotoxicology 6, 361–370. [DOI:10.3109/17435390.2011.579632]
32. Zhao C.M. and Wang W.X. (2013) Regulation of sodium and calcium in Daphnia magna exposed to silver nanoparticles. Environmental Toxicology and Chemistry 32, 913–919. [DOI:10.1002/etc.2133]

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Tulaby Dezfuly Z, Alishahi M, Aramoon A, Mashjoor S. Evaluation of silver nanoparticles toxicity in Daphnia magna: Comparison of chemical and green biosynthetic productions. I. j. Aqua. Anim. Health. 2017; 3 (2) :74-85
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