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:: دوره 3، شماره 2 - ( 5-1396 ) ::
جلد 3 شماره 2 صفحات 98-109 برگشت به فهرست نسخه ها
ارزیابی فعالیت کمپلمان در سرم چهار گونه از ماهیان خاویاری: اوزون برون (Acipenser stellatus)، سیبری (Acipenser baeri)، شیپ (Acipenser nudiventris) و فیل ماهی (Huso huso)
آزاده یکتا سرشت ، امین غلامحسینی، علی جانپرور
چکیده:   (641 مشاهده)
Serum complement is valuable tool in determining the health status of fish. This study was conducted to examine in vitro activity of serum complement as an indicator of innate immunity in four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae). The Effects of different temperatures, concentrations and volumes of serum on alternative complement pathway activity were evaluated using standard haemolytic assays in four sturgeon species (A. stellatus, A baeri, A. nudiventris and Huso huso). Results indicated significant relation between measured hemolysis and concentration, volume and temperature (p<0.05). Maximal hemolytic activity was exhibited at 100% serum in four sturgeon species. (p<0.05). Exposure of different volumes of serum from sturgeon species to RBCs exhibited volume dependent hemolysis (p<0.05). Maximal hemolytic activity was resulted in 30 μL of serum for all the sturgeon species. The highest hemolytic activity occurred at 4 °C for all the sturgeon species, but the hemolytic activity decreased at 37 °C. The results showed that the alternative complement pathway activity in sturgeon species serum may be affected by temperature, concentration and volume of serum that it caused an increase in non-specific immunity and the resistance to the outbreak of diseases.

 
 
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نوع مطالعه: پژوهشي | موضوع مقاله: Aquaculture and Health management
دریافت: ۱۳۹۶/۵/۲۸ | پذیرش: ۱۳۹۶/۱۰/۴ | انتشار: ۱۳۹۶/۱۰/۱۱


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Yektaseresht A, Gholamhosseini A, Janparvar A. Characterization of complement activity in serum of the four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae) . I. j. Aqua. Anim. Health. 2017; 3 (2) :98-109
URL: http://ijaah.ir/article-1-148-fa.html

یکتا سرشت آزاده، غلامحسینی امین، جانپرور علی. ارزیابی فعالیت کمپلمان در سرم چهار گونه از ماهیان خاویاری: اوزون برون (Acipenser stellatus)، سیبری (Acipenser baeri)، شیپ (Acipenser nudiventris) و فیل ماهی (Huso huso). نشریه ایرانی سلامت حیوانات آبزی. 1396; 3 (2) :98-109

URL: http://ijaah.ir/article-1-148-fa.html



دوره 3، شماره 2 - ( 5-1396 ) برگشت به فهرست نسخه ها
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