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I. j. Aqua. Anim. Health 2017, 3(2): 98-109 Back to browse issues page
Characterization of complement activity in serum of the four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae)
A Yektaseresht , A Gholamhosseini , A Janparvar
Abstract:   (1078 Views)
Serum complement is valuable tool in determining the health status of fish. This study was conducted to examine in vitro activity of serum complement as an indicator of innate immunity in four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae). The Effects of different temperatures, concentrations and volumes of serum on alternative complement pathway activity were evaluated using standard haemolytic assays in four sturgeon species (A. stellatus, A baeri, A. nudiventris and Huso huso). Results indicated significant relation between measured hemolysis and concentration, volume and temperature (p<0.05). Maximal hemolytic activity was exhibited at 100% serum in four sturgeon species. (p<0.05). Exposure of different volumes of serum from sturgeon species to RBCs exhibited volume dependent hemolysis (p<0.05). Maximal hemolytic activity was resulted in 30 μL of serum for all the sturgeon species. The highest hemolytic activity occurred at 4 °C for all the sturgeon species, but the hemolytic activity decreased at 37 °C. The results showed that the alternative complement pathway activity in sturgeon species serum may be affected by temperature, concentration and volume of serum that it caused an increase in non-specific immunity and the resistance to the outbreak of diseases.

 
 
Keywords: Complement, Hemolysis, Immunity, Serum, Sturgeon
Full-Text [PDF 662 kb]   (311 Downloads)    
Type of Study: Research | Subject: Aquaculture and Health management
Received: 2017/08/19 | Accepted: 2017/12/25 | Published: 2018/01/1
References
1. Birstein V.J. (1993) Sturgeon and Paddlefishes: Threatened Fishes in Need of Conservation. Conservation Biology 7 (4), 773-787. [DOI:10.1046/j.1523-1739.1993.740773.x]
2. Buchtikova S., Simkova A., Rohlenov K., Flajshans M., Lojek A., Lilius E.M., Hyrsl P.(2011) The seasonal changes in innate immunity of the common carp (Cyprinus carpio). Aquaculture 318, 169–175. [DOI:10.1016/j.aquaculture.2011.05.013]
3. Gongora R., Figueroa F., Klein J. (1998) Independent duplication of Bf and C3 complement genes in the zebrafish. Scandinavian Journal of Immunology 48, 651-658. [DOI:10.1046/j.1365-3083.1998.00457.x]
4. Hedrick R.P., McDowell T.S., Ahne W., Torhy C., Kinkelin P.d. (1992) Proerties of three iridovirus-like agents associated with systemic infections of fish. Diseases of Aquatic Organisms 13, 203-209. [DOI:10.3354/dao013203]
5. Hernandez A., Tort L. (2003). Annual variation of complement. lysozyme and haemagglutinin levels in serum of the gilthead sea bream Sparus aurata. Fish and Shellfish Immunology15, 479–481. [DOI:10.1016/S1050-4648(03)00024-X]
6. Holland M.C.H, Lambris J.D. (2002) The complement system in teleosts. Fish and Shellfish Immunology 12, 399-420. [DOI:10.1006/fsim.2001.0408]
7. Ingram G.A. (1987) Haemolytic activity in the serum of brown trout, Salmo trutta L. Journal of Fish Biology, 31, 9-17. [DOI:10.1111/j.1095-8649.1987.tb05286.x]
8. Kirschfink M., Mollnes T.E. (2003) Modern complement analysis. Clinical and Diagnostic Laboratory Immunology 10, 982-989. [DOI:10.1128/CDLI.10.6.982-989.2003]
9. Kuroda N., Naruse K., Shima A., Nonaka M., Sasaki M., Nonaka M. (2000) Molecular cloning and linkage analysis of complement C3 and C4 genes of the Japanese medaka fish. Immunogenetics 51, 117-128. [DOI:10.1007/s002510050020]
10. Lachmann P.J., Hobart M.J. (1978) Complement technology. In: Handbook of experimental immunology (ed. By D. M. Weir), pp.5A.12–5A.13. Blackwell Scientific Publications, Oxford.
11. LaPatra S.E., Groff J.M., Jones G.R., Munn B., Patterson T.L., Holt R.A., Hauck A.K., Hedrick R.P. (1994) Occurrence of white sturgeon iridovirus infections among cultured white sturgeon in the Pacific Northwest. Aquaculture 126, 201-210. [DOI:10.1016/0044-8486(94)90036-1]
12. Matsuyama H., Hirata A., Nakao M., Yano T. (1985) Optimum conditions for the assay of hemolytic complement titer of porgy (Pagrms major) serum. Journal of the Faculty of Agriculture Kyushu University 30, 149-158.
13. Merchant M., Britton A. (2006) Characterization of serum complement activity of the saltwater (Crocodylus porosus) and freshwater (Crocodylus johnstoni) crocodiles. Comparative Biochemistry and Physiology A 143, 488-493. [DOI:10.1016/j.cbpa.2006.01.009]
14. Merchant M.E., Henry D., Falconi R., Muscher B., Bryja J. (2012) Characterization of serum complement activity in serum of the Komodo dragon (Varanus komodoensis). Advances in Biological Chemistry 2, 353-359. [DOI:10.4236/abc.2012.24043]
15. Meng F., Sun Y., Liu X., Wang J., Xu T., Wang R. (2012) Analysis of C3 Suggests Three Periods of Positive Selection Events and Different Evolutionary Patterns between Fish and Mammals. PLoS ONE 7(5), e37489. [DOI:10.1371/journal.pone.0037489]
16. Montgomery J.M., Gillespie D., Sastrawan P., Freeking T.M.,Stewart G.L. (2002) Aerobic salivary bacteria in wild and captive Komodo dragons. Journal of Wildlife Diseases 38,545- 551. [DOI:10.7589/0090-3558-38.3.545]
17. Nakao M., Mutsuro J., Obo R., Fujiki K., Nonaka M., Yano T. (2000) Molecular cloning and protein analysis of divergent forms of the complement component C3 from a bony fish, the common carp (Cyprinus carpio): presence of variants lacking the catalytic histidine. European Journal of Immunology 30, 858-866. [DOI:10.1002/1521-4141(200003)30:33.0.CO;2-M]
18. Nakao M., Obo R., Mutsuro J., Yano T. (1997) Sequence diversity of cDNA encoding the third component (C3) of carp (Cyprinus carpio). Developmental & Comparative Immunology 21, 144. [DOI:10.1016/S0145-305X(97)88612-0]
19. Nakao M., Yano T. (1988) Characterization of the Classical Complement Pathway of Rainbow Trout. Journal of the Faculty of Agriculture Kyushu University 33, 61-65.
20. Nakao M., Yano T. (1998) Structural and functional identification of complement components of the bony fish, carp (Cyprinus carpio). Immunological Review166, 27-38. [DOI:10.1111/j.1600-065X.1998.tb01250.x]
21. Pridgeon J.W., Klesius P.H. (2012) Major bacterial diseases in aquaculture and their vaccine development CAB Reviews 7, 048.
22. Sakai, D.K. (1981) Heat inactivation of complements and immune hemolysis reactions in rainbow trout, masu salmon, coho salmon, goldfish and tilapia. Bulletin of the Japanese Society of Scientific Fisheries 47, 565-571. [DOI:10.2331/suisan.47.565]
23. Soltani M., Kalbassi M.R. (2001) Protection of Persian sturgeon (Acipenser persicus) fingerling against Aeromonas hydrophila septicemia using three different antigens. Bulletin of the European Association of Fish Pathologists 21, 235-239.
24. Sunyer J.O., Tort L., Lambris J.D. (1997) Diversity of the third form of complement, C3, in fish: functional characterization of five forms of C3 in the diploid fish Sparus aurata. Biochemical Journal 326, 877-881. [DOI:10.1042/bj3260877]
25. Sunyer J.O., Zardakis I.K., Sahu A., Lambris J.D. (1996) Multiple forms of complement C3 in trout that differ in binding to complement Activators. Proceedings of the National Academy of Sciences of the United States of America 93, 8546-8551. [DOI:10.1073/pnas.93.16.8546]
26. Smith L.C., Azumi K., Nonaka M. (1999) Complement systems in invertebrates. The ancient lectin and alternative Pathways. Immunopharmacology l42, 107–120.
27. Takahashi, J.D.B., Takahashi, L.S., Machado, C.M.M., Sabbadin F., Sabioni Z.R.E., Criscuolo E. (2012) Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus). Revista Brasileira de Zootecnia 41, 237-241. [DOI:10.1590/S1516-35982012000200001]
28. Watson L.R., Yun S.C., Groff J.M., Hedrick R.P. (1995) Characteristics and pathogenicity of a novel herpesvirus isolated from adult and subadult white sturgeon Acipenser transmontaneus. Diseases of Aquatic Organisms 22, 199-210. [DOI:10.3354/dao022199]
29. Watson L.R., Groff J.M., Hedrick R.P. (1998) Replication and pathogenesis of white sturgeon iridovirus in experimentally infected white sturgeon Acipenser transmontaneus juvenile sand sturgeon cell lines. Diseases of Aquatic Organisms 32, 173-184. [DOI:10.3354/dao032173]
30. Yano T., Ando H., Nakao M. (1984) Optimum conditions for the assay of hemolytic complementtiter of carp and seasonal variation of the titers. Journal of the Faculty of Agriculture Kyushu University 29, 91-101.
31. Yano T., Hatayama Y., Matsuyama H., Nakao M. (1988) Optimum conditions for the assay of the classical pathway-complement titer of tilapia (Tilapia nilotica) serum. Journal of the Faculty of Agriculture Kyushu University, 33, 29-36.
32. Yano T. (1992) Assays of hemolytic complement activity. In: Techniques in fish immunology (ed. by Stolen J.S., Fletcher T.C., Anderson D.P., et al.), pp.131-141. Fair Haven, New Jersey: SOS.
33. Yektaseresht A., Gholamhosseini A., Janparvar A., Salighehzadeh R. (2016) Effect of concentration, volume and temperature of serum complement of common carp (Cyprinus carpio), on unsensitized rabbit red blood cell hemolysis. Online Journal of Veterinary Research 20(8), 557-562.
34. Yektaseresht A., Gholamhosseini A., Janparvar A. (2017) Evaluation of the effect of temperature, concentration and volume of serum complement on alternative complement pathway activity in koi carp (Cyprinus carpio koi). Specialty Journal of Biological Sciences 3 (1), 1-6.



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Yektaseresht A, Gholamhosseini A, Janparvar A. Characterization of complement activity in serum of the four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae) . I. j. Aqua. Anim. Health. 2017; 3 (2) :98-109
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