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I. j. Aqua. Anim. Health 2018, 4(1): 49-58 Back to browse issues page
Histological Description of intestine and Gut Associated Lymphoid Tissue in Esox Lucius from Anzali wetland
N Salamat
Abstract:   (527 Views)
Study of gut tissue structure in fish is important as the fish culture increases and then, more information is required regarding to feeding and nourishment. Then, the present study aimed to describe the histological and histochemical characteristics of GALT in the pike, Esox lucius, from the Anzali wetland. In this regards, 20 E. lucius were collected from the Anzali wetland. Tissue samples from different parts of gut were fixed in Bouin’s solution. Tissue slides were prepared using tissue preparation techniques and stained with hematoxylin and eosin (H&E), Periodic acidSchiff (PAS), Alcian blue and AB-PAS. In E. lucius the internal surface of gut was folded and coated by simple columnar cells and goblet cells. Goblet cells positively reacted to PAS, AB and AB-PAS, due to mix acidic and neutral glycoproteins containing. Eosinophilic granular cells were detected in lamina propria. GALT represented throughout the gut mucosa in the epithelium and lamina propria. Lymphocytes increased in number toward the posterior part of intestine (P<0.05).

Keywords: Gut, GALT, Esox lucius, Histology, Anzali wetland.
Full-Text [PDF 565 kb]   (137 Downloads)    
Type of Study: Research | Subject: Histopathology
Received: 2017/12/11 | Accepted: 2018/03/17 | Published: 2018/10/21
1. Abelli L., Picchietti S., Romano N., Mastrolia L., Scapigliati G. (1997) Immunohistochemistry of gut-associated lymphoid tissue of the sea bass Dicentrarchus labrax (L.). Fish and Shellfish Immunology 7, 235-245. [DOI:10.1006/fsim.1996.0079]
2. Al-Abdulhadi H.A. (2005) Some comparative histological studies on alimentary tract of tilapia fish (Tilapia spiluru) and sea bream (Mylio cuveir). Egyptian Journal of Aquatic Research 31, 387-397.
3. Banan khojasteh S.M., Sheikhzadeh F., Mohammanejad D., Azami A. (2009) Histological, histochemical and ultrastructural study of the intestine of Rainbow Trout (Oncorhynchus mykiss). World Applied Science 6(11), 1529-1531.
4. Brandtzaeg P., Pabst R. (2004) Let's go mucosal: communication on slippery ground. Trends in Immunology 25, 570-577. [DOI:10.1016/j.it.2004.09.005]
5. Brandtzaeg P., Kiyono H., Pabst R., Russell M.W. (2008) Terminology: nomenclature of mucosa-associated lymphoid tissue. Mucosal Immunology 31. [DOI:10.1038/mi.2007.9]
6. Carrasson M., Grau A., Dopazo L.R., Crespo S. (2006) A histological, histochemical and ultrastructural study of the digestive tract of Dentex dentex (pisces, sparidae). Histology and Histopathology 21(6), 579-593.
7. Cerutti A. (2008) The regulation of IgA class switching. Nature Reviews Immunology 434. [DOI:10.1038/nri2322]
8. Cheroutre H., Madakamutil L. (2005) Mucosal effector memory T cells: the other side of the coin. Cellular and Molecular Life Sciences 62, 2853-2866. [DOI:10.1007/s00018-005-5232-y]
9. Cinar K., Senol N. (2006) Histological and histochemical characterization of the mucosa of the digestive tract in flower fish (Pseudophoxinus antalyae). Anatomia, Histologia, Embryologia 35, 147-151. [DOI:10.1111/j.1439-0264.2005.00629.x]
10. Dai X., Shu M., Fang W. (2007) Histological and ultrastructural study of the digestive tract of rice field eel, Monopoterus albus. Applied Ichthyology 23, 177-183. [DOI:10.1111/j.1439-0426.2006.00830.x]
11. Doggett T.A., Harris J.E. (1991) Morphology of the gut associated lymphoid tissue of Oreochrotnis tnossambicus and its role in antigen absorption. Fish and Shellfish Immunology 1, 213-227. [DOI:10.1016/S1050-4648(10)80006-3]
12. Hernandez D.R., Pérez Gianeselli M., Domitrovic M. (2009) Morphology, Histology and Histochemistry of the Digestive System of South American Catfish (Rhamdia quelen). International Journal of Morphology 7, 5-111. [DOI:10.4067/S0717-95022009000100019]
13. Kucharzik T., Lugering N., Rautenberg K., Lugering A., Schmidt M.A., Stoll R., Domschke W. (2000) Role of M cells in intestinal barrier function. Annals of the New York Academy Science 915, 171-183. [DOI:10.1111/j.1749-6632.2000.tb05240.x]
14. Kumar S., Tembhre M. (1996) Anatomy physiology of fishes. 1st Edn. Nehru Nagar, Ghaziabad, Vikas Publishing House PVT Ltd,70.
15. Martin T.J., Blaber S.J.M. (1984) Morphology and histology of the alimentary tracts of Ambassidae (Cuvier) (Teleostei) in relation to feeding. Morphology 182, 295-305. [DOI:10.1002/jmor.1051820305]
16. Murry H.M., Wright G.M., Goff G.P. (1994) A comparative histological and histochemical study of the stomach from three species of pleuronectid, the Atlantic halibut, Hippoglossus hippoglussus, the yellowtail flounder, Pleuronecles americanus. Canadian Journal of Zoology 72, 1199-1210. [DOI:10.1139/z94-161]
17. Nikbakht M., Albughobeysh N., Peyghan R. (2007) Histological and Histometerical study of Gut Associated Lymphoid Tissue of intestinal bulb and proper intestine in Barbus sharpeyi. Science Journal of Shahid Chamran University 17, 106-116.
18. Piccheietti S., Terribili F.R., Mastrolia L., Scapigliati G., Abelli L. (1997) Expression of lymphocyte antigenic developing Gut – Associated Lymphoid Tissue the sea bass (Dicentrarchus Labrax L.). Anatomy and Embryology 196(6), 457-463. [DOI:10.1007/s004290050113]
19. Raji A.R., Norouzi E. (2010) Histological and histochemical study on the alimentary canal in Walking catfish (Claris batrachus) and piranha (Serrasalmus nattereri). Iranian Journal of Veterinary Research 11(3), 255-261.
20. Reid P.E., Volz D., Cho K.Y., Owen D.A. (1988) A new method for the demonstration of oacy sugar in human colonic histochemical epithelial glycoproteins. Histochemical Journal 20, 510-518. [DOI:10.1007/BF01002649]
21. Reite O.B. (1997) The rodlet cells of teleosts: evidence of activation in tissues exposed to noxious agents. In: Abstracts of the 8th Conference of the European Association of Fish Pathologists, Edinburgh, 12.
22. Reite O.B., Evensen O. (2006) Inflammatory cells of teleostean fish: A review focusing on mast cells/eosinophilic granule cells and rodlet cells. Fish and Shellfish Immunology 20, 192-208. [DOI:10.1016/j.fsi.2005.01.012]
23. Rombout J.H.W.M., Taverne N., Van de Kamp M., Taverne-Thiele A.J. (1993a) Differences in mucus and serum immunoglobulin of carp (Cyprinus carpio L.). Developmental and Comparative Immunology 17, 309-317. [DOI:10.1016/0145-305X(93)90003-9]
24. Rombout J.H.W.M., Taverne-thiele A.J., Villena M.L. (1993b) The Gut- Associated Lymphoid Tissue (GALT) of carp (Cyprinus carpio L.): An immunocytochemical analysis. Developmental and Comparative Immunology 17, 55-66. [DOI:10.1016/0145-305X(93)90015-I]
25. Rombout J.H.W.M., Abelli L., Picchietti S., Scapigliati G., Kiron V. (2010) Teleost intestinal immunology, Fish and Shellfish Immunology 32, 1-11.
26. Stoskopf M.K. (1993) Fish medicine. Saunders Company, 13.
27. Temkin R.J., McMillan D.B. (1986) Gut associated lymphoid tissue (GALT) of goldfish Carassius auratus. Journal of Morphology 190, 9-26. [DOI:10.1002/jmor.1051900103]

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Salamat N. Histological Description of intestine and Gut Associated Lymphoid Tissue in Esox Lucius from Anzali wetland. I. j. Aqua. Anim. Health. 2018; 4 (1) :49-58
URL: http://ijaah.ir/article-1-155-en.html

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