One of the most important and influential stress causing problem and secondary diseases in fish is transport stress. The aim of this study was to evaluate the physiological effects of acute stress of fish transportation on some biochemicals in Salmo trutta caspius. A total of 100 fish were transported in plastic bags for 6 h and then released in 300-l tanks. Blood samples were taken after 6, 12, 24 and 48 h after a 6-h transportation (n=15). Based on the results, blood glucose increased compared to the basal value (p<0.05) after 6 h but the value was decreased at 12 and 24 h compared to that of 6 h. Cortisol value was increased significantly (p<0.05) in all sampling times.  Unexpectedly, protein content was significantly increased (p<0.05) at 24h. On the other hands, other parameters uch as Na⁺, Cl^-, K⁺, did not show a significant  variation after transportation (p>0.05).