[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: ::
Back to the articles list Back to browse issues page
Effect of garlic peel on haematological, biochemical and digestive enzyme activity in beluga juvenile (Huso huso)
Hا Chitsaz , H Oraji , A Keramat Amirkolaei , R Akrami
Abstract:   (851 Views)
Our study was concentrated on the effect of garlic peel on haematological parameters, biochemistry and digestive enzymes on beluga juvenile (Huso huso) with mean body weight of 18. 41 ± 0. 89 g after the feeding trial for 90 days. Garlic peel powder added with 0%, %0.5, %1, %1.5 and %2 of feed to basic sturgeon diet (49% protein and 14% lipid). ultimately, haematological parameters, biochemistry, digestive enzyme activity were evaluated. Results showed that the hemoglobin (Hb) was remarkably greater in the treatment fed garlic peel diet in comparison with the control (p<0.05), while others blood indices did not significant differences (p>0.05) between juveniles fed control and garlic peel supplementation diets. The group fed 1.5% garlic peel showed highly significant different in total protein (p<0.05). However, Glucose, triglyceride, total lipid, cholesterol, ALP and ALT were significantly decrease in the juvenile fish fed by the 1.5% garlic peel diet compare to the control (p<0.05).The fish treated with 1.5% garlic peel exhibited significantly increase in amylase, protease and lipase compare to the control (p<0.05), but trypsin, chymotrypsin and  pepsin were not affected (p>0.05). These results indicated that garlic peel powder in 1.5% level, improved blood indices and digestive enzymes activity of beluga sturgeon.
 
Keywords: Garlic peel, Blood indices, Digestive enzyme, Huso huso
Full-Text [PDF 621 kb]   (306 Downloads)    
Type of Study: Applicable | Subject: hematology
Received: 2017/08/8 | Accepted: 2018/03/8 | Published: 2018/03/8
References
1. Abdel-Fattah A. F., Khaireldin A. (1970) Pectin of garlic skins. U. A. R. J. Chem. 13, 27-37.
2. Abdel-Fattah A. F., Edrees M.(1972) A study on the composition of garlic skins and the structural features of the isolated pectic acid. J of Sci. Food Agric. 23, 871-877. [DOI:10.1002/jsfa.2740230708]
3. Alexander M. M., Sulebele G.A. (1973) Pectic substances in onion and garlic skins. J of Sci. Food Agric. 24, 611-615. [DOI:10.1002/jsfa.2740240514]
4. Akrami R., Nasritajan M., Jahedi A., Jahedi M.,Razeghi Mansour M., Jafarpour S.A. (2015a) Effects of dietary Synbiotic on growth, survival,lactobacillus bacterial, blood indices and immunity of beluga (Huso huso) juvenile.Aquaculture Nutrition 21(6), 952–959. [DOI:10.1111/anu.12219]
5. Akrami R., Gharaei A., Razeghi Mansour M. Galeshi A. (2015b) Effects of dietary onion (Allium cepa) powder on growth, innate immune response and haemato-biochemical parameters of beluga (Huso huso) juvenile. Fish and Shellfish Immunology 45 (2), 828–834. [DOI:10.1016/j.fsi.2015.06.005]
6. Bessey OA., Lowry OH., Brock MJ. (1946) Rapid coloric method for determination of alkaline phosphatase in five cubic millimeters of serum. J Biolog Chem 164,321–329
7. Bhardwaj N., Strickland AD., Ahmad F., Atanesyan L., West K., Lloyd DM. (2009) A comparative histological evaluation of the ablations produced by microwave, cryotherapy and radiofrequency in the liver. Pathology 41(2):168–172. [DOI:10.1080/00313020802579292]
8. Binaii M., Ghiasi M., Farabi S.M., Pourgholam R., Fazli H., Safari R., Alavi S.E., Taghavi M.J., Bankehsaz Z. (2014) Biochemical and haemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica). Fish Shellfish Immunol 36(1).46- 51. [DOI:10.1016/j.fsi.2013.10.001]
9. Bradford M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72,248–254. [DOI:10.1016/0003-2697(76)90527-3]
10. Chitsaz H., Akrami R. (2016) Effect of garlic peel on metabolic enzymes activity in common carp (Cyprinus carpio) The first national conference of aromatic and medicinal herbs Gonbad kavous university.
11. Collier H.B. (1944) The standardization of blood hemoglobin determinations. Can. Med. Assoc. J., 50, 550–552.
12. Ewuola E.o., Folayan o.A., Gbore F.A., Adebunmi Akanji R.A., Ogunlade J.T., Adeneye J.A. (2004) Physiological response of growing West African dwarf goats fed groundnut shell-based diets as the concentrate supplements Bowen Journal of Agriculture 1, 61-69.
13. Fazlolahzadeh F., Keramati K., Nazifi S., Shirin S., Seifi S. (2011) Effect of garlic (Allium sativum) on haematological parameters and plasma activities of ALT and AST of Rainbow trout (oncorhynchus mykiss) in temperature stress. Australian Journal of Basic and Applied Sciences 5(9), 84-90.
14. Frankic T., Voljc M., Salobir J., Rezar V.(2009) Use of herbs and spices and their extracts in animal nutrition. Acta agriculturae Slovenica. 94(2),95-102.
15. Ghiasi F., Mirzargar SS., Badakhshan H., Shamsi S. (2010) Effects of low concentration of cadmium on the level of lysozyme in serum, leukocyte count and phgocytic index in Cyprinus carpio under the wintering conditions. J. Fisheries Aquat. Sci. 5(2), 113-119. [DOI:10.3923/jfas.2010.113.119]
16. Gholipour Kanani H., Nobahar Z., Kakoolaki Sh., Jafarian H. (2014) Effect of ginger- and garlic-supplemented diet on growth performance, some haematological parameters and immune responses in jvenile Huso huso. Fish Physiol Biochem 40,481–490. [DOI:10.1007/s10695-013-9859-6]
17. Gisbert E., Giménez G., Fernández I., Kotzamanis Y. Estévez A. (2009) Development of digestive enzymes in common dentex, Dentex dentex during early ontogeny.Aquaculture, 287: 381-387. [DOI:10.1016/j.aquaculture.2008.10.039]
18. Goldenfarb P.B., Bowyer F.P., Hall E., Brosious E. (1971) Reproducibility in the haematology laboratory: the microhaematocrit determination. J. Clin. Pathol. 56, 35–39. [DOI:10.1093/ajcp/56.1.35]
19. Harada K. (1990) Attraction activities of spices for oriental weather fish and yellowtail. Bull Jap Soc Sci Fish. 56, 2029-2033. [DOI:10.2331/suisan.56.2029]
20. Hassanpour M. (2015) Effect of Dietary Ginger (Zingiber officinale) Extract on Growth, Biochemical and Immunological Parameters in Juvenile Huso huso, MSc. Thesis, Khazar Institute of Higher Education (Nonprofit- Nongovernment),Mahmoudabad,Iran pp:124
21. Hoseinifar S.H., Mirvaghefi A., Merrifield D.L., (2011) The effects of dietary inactive brewer's yeast Saccharomyces cerevisiae var. ellipsoideus on the growth, physiological responses and gut microbiota of juvenile beluga (Huso huso).Aquaculture nutrition 25,354-362. [DOI:10.1016/j.aquaculture.2011.04.043]
22. Ifesan B. O. T., Fadipe E. A., (2014) Investigation of Antioxidant and Antimicrobial Properties of Garlic Peel Extract (Allium sativum) and Its Use as Natural Food Additive in Cooked Beef. Journal of Scientific Research and Reports. 3 (5) . [DOI:10.9734/JSRR/2014/5726]
23. Iijima N., Tanaka S., Ota Y., (1998) Purification and characterization of bile salt–activated lipase from the hepatopancreas of red sea bream, Pagrus major. Fish Physiology and Biochemistry, 18, 59–69. [DOI:10.1023/A:1007725513389]
24. Jha AK., Pal A.K., Sahu N.P., Kumar S. Mukherjee S.C. (2007) Haematoimmunological responses to dietary yeast RNA,w-3fatty acid and b-carotene in Catla catla juveniles. Fish & Shellfish Immunology 23,917–927. [DOI:10.1016/j.fsi.2007.01.011]
25. Jian J., Wu Z. (2003) Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker (Pseudosciaena crocea). Aquaculture, 218, 1–9. [DOI:10.1016/S0044-8486(02)00192-8]
26. Klontz G.W. (1994) Fish haematology. Techniques in Fish Immunology, vol. 3, pp. 121–132.
27. Langlois A., Corring T., Fevrier C. (1987) Effects of wheat bran on exocrine pancreas secretion in the pig. Reprod Nutr Dev 27,929–939 [DOI:10.1051/rnd:19870705]
28. Lee JY., Gao Y.(2012) Review of the application of garlic, Allium sativum, in aquaculture. J World Aquac Soc, 43, 447-458. [DOI:10.1111/j.1749-7345.2012.00581.x]
29. Martin-Kleiner I., Flegar-Mestric Z., Zadro R., Breljak D., Stanovic Janda S., Stojkovic R., Marusic M., Radacic M& Boranic M. (2001) The effect of the zeolite clinoptilolite on serum chemistry and hematopoiesis in mice. Food Chem. Toxicol. 39(7), 717-27. [DOI:10.1016/S0278-6915(01)00004-7]
30. Mohseni M., Pourkazemi M., Bahmani M., Pourali H.R., Sajjadi M.M. (2007) Effect of different dietary protein to energy ratios (P/E) on growth performance and body composition of farmed persian sturgeon (Acipenser persicus).Iranian scientific fisheries journal.16(1).
31. Nya E.J. Austin B. (2009) Use of dietary ginger, Zingiber officinale Roscoe as an immunostimulant to control Aeromonas hydrophila infections in rainbow trout,Oncorhynchus mykiss (walbaum). Journal of Fish. Diseases 32(11), 971-977. [DOI:10.1111/j.1365-2761.2009.01101.x]
32. Patriche T., Patriche N., Tenciu M. (2009),Cyprinids total blood proteins determination,Lucrări ştiinţifice Zootehnie şi Biotehnologii 42(2), 95-101 , Timişoara.
33. Platel K., Rao A., Saraswah G., Srinivasan K. (2002) Digestive stimulant action of three indian spice mixes in experimental rats. Die Nahrung. 46,394-398. https://doi.org/10.1002/1521-3803(20021101)46:6<394::AID-FOOD394>3.0.CO;2-D [DOI:10.1002/1521-3803(20021101)46:63.0.CO;2-D]
34. Pourkazemi M., (2006) Caspian Sea sturgeon conservation and fisheries: Past, Present and Future. Journal of Applied Ichthyology 22, 12-16. [DOI:10.1111/j.1439-0426.2007.00923.x]
35. Rungraungsak-Torrissen K., Sunde J., Berg A.E., Nordgarden U., Fjelldal P.G. Oppedal F. (2008) Digestive efficiency, free amino acid pools and quality of growth performance in Atlantic salmon (Salmo salar L.) affected by light and vaccine type. Fish Physiology and Biochemistry 35 (2), 255-272. [DOI:10.1007/s10695-008-9206-5]
36. Safarpour Amlashi A., Falahatkar B., Sattari M., Tolouei Gilani MH. (2011) Effect of di-etary vitamin E on growth, muscle composition, haematological and immu-nological parameters of sub-yearling beluga Huso huso L. Fish & Shellfish Immunology 30,807-14. [DOI:10.1016/j.fsi.2011.01.002]
37. Sakai M. 1999. Current research status of fish immunostimulants. Aquaculture 172, 63–92. [DOI:10.1016/S0044-8486(98)00436-0]
38. Sankar H., Philip B., Philip R. (2017) Effect of probiotics on digestive enzyme activities and growth of cichlids,Etroplus suratenis (Pearl spot) and Oreochromis mossambicus(Tilapia).Aquaculture Nutririon Journal 23,852-864. [DOI:10.1111/anu.12452]
39. Schmidtlein H., Herrmann K. (1975) On the phenolic acids of vegetables. IV. Hydroxycinnamic acids and hydroxybenzoic acids of vegetables and potatoes. Z. Lebensm. Unters.-Forsch 159, 255-263. [DOI:10.1007/BF01139577]
40. Shubha Ratna Sh.(2017) Effect of Herbs and Herbal Products Feed Supplements on Growth in Fishes: A Review. Nepal Journal of Biotechnology Vol. 5, 1, 58-63. [DOI:10.3126/njb.v5i1.18870]
41. Sunde J., Taranger G.L., Rungruangsak-Torrissen K. (2001) Digestive protease activities and free amino acids in white muscle as indicators for feed conversion efficiency and growth rate in Atlantic salmon (Salmo salarL.) Fish Physiol. Biochem. 25,335–345. [DOI:10.1023/A:1023233024001]
42. Talpur A.D., Ikhwanuddin M., Ambok Bolong A. (2013) Nutritional effects on ginger (Zingiber officinal Roscoe) on immune response of Asian sea bass (Lates calcarifer) and disease resistance against Vibrio harveyi. Aquacultur 46, 400–401.
43. Thanikachalam K., Kasi M., Rathinam X. (2010) Effect of garlic peel on growth, haematological parameters and disease resistance against Aeromonas hydrophila in African catfish Clarias gariepinus(Bloch) fingerlings. Asian Pacific Journal of Tropical Medicine. 614-618. [DOI:10.1016/S1995-7645(10)60149-6]
44. Tras Brown K.M. (2007) Fish applied pharmacology, translated by:Fatemi M., Mirzargar s.Tehran University Press.first publish.
45. Vahedi AH., Hasanpour M., Akrami R., Chitsaz H. (2017) Effect Of Dietary Supplementation With Ginger (Zingiber Officinale) Extract On Growth, Biochemical And Hemato-immunological Parameters In Juvenile Beluga (Huso Huso),Iranian journal of aquatic animal health, Volume 3, Issue 1 (4).
46. Vaseeharan B., Thaya R. (2013) Medicinal plant derivatives as immunostimulants. an alternative to chemotherapeutics and antibiotics in aquaculture. Aquac. Int.
47. Walter H. (1984) Proteinases methods with hemoglobin, casein and azocoll as substrates. In Bergmeyer HU (ed) Methods of enzymatic analysis, vol V. Verlag Chemie, Weinheim,pp 270–277.
48. Wilson A., Demmig-Adams B. (2007) Antioxidant, anti-inflammatory, and antimicrobial properties of garlic and onions, nutrition and food science. 27(3).
49. Windisch W., Schedle K., Plitzner C., Kroismayer A. (2008) Use of phytogenetic products as feed additives for swine and poultry. Journal of Animal Science 86 (Supplement): E140–E148. [DOI:10.2527/jas.2007-0459]
50. Yano T. (1992) Assay of hemolytic complement activity. In: J.S. Stolen, T.C. Fletcher, D.P. Anderson, S.C. Hattari, A.F. Rowley (Eds.). Techniques in fish immunology. SOS Publications, Fair Haven, New Jersey, USA. 2.131-141.
51. Zambonino JL., Cahu CL. (1994) Influence of diet on pepsin and some pancreatic enzymes in sea bass (Dicentrarchus labrax) larvae. Comp Biochem Physiol 109,209–212. [DOI:10.1016/0300-9629(94)90122-8]


XML     Print



Back to the articles list Back to browse issues page
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 3767