1 2821-1383 Iranian Association of Aquatic Animal Health 110 hematology Effect of different stocking densities on hematological parameters and growth performance of great sturgeon (Huso huso) Naderi Samani M b Jafaryan H c Jafaryan S d b 1Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran c 2Department of Fishery, Gonbad University of Agriculture Sciences and Naturar Resources, Gonbad, Iran. d 2Department of Fishery, Gonbad University of Agriculture Sciences and Naturar Resources, Gonbad, Iran. 1 11 2017 3 2 1 10 29 06 2016 24 06 2017 A 5 weeks study was conducted to determine the effects of different stocking density on hematological parameters of great sturgeon. Fish were cultured in duplicates of fiberglass tanks under stocking densities of 50, 75, 100 and 125 fish/m3 as T1, T2, T3 and T4 respectively. Based on the results significant increase (p<0.05) in total body weight and total length were related to low density group. The lowest mean body weight (103.26 gr) and length (29.65 cm) were recorded in T4. At the end of experiment, Results of hematological analyses showed changes in the stocking density had non-significant impact on values of WBC and mean corpuscle hemoglobin (MCH) (p>0.05). The highest mean corpuscular hemoglobin concentration (MCHC) was also recorded in T4. The highest level of hemoglobin, hematocrit (7.25 g dl-1 and 22.2%) were significantly associated with T1 (p<0.05). The findings suggested that the haematological parameters and growth performance are considered as a benefactor for indicating stress in fish due to different physical or chemical parameters.
129 Histopathology Effects of salinity and plasma prolactin on chloride cells in the gill of Chalcalburnus chalcoides Neurasteh N e Setorki m f Tehranifard A g Moshfegh A h e islamic azad u f islamic azad u g islamic azad u h islamic azad u 1 11 2017 3 2 11 21 26 02 2017 31 07 2017 Gill chloride cells and prolactin hormone are of high importance in the adaptation of euryhaline fish. Guldenstati (Chalcalburnus chalcoides, 1772), an adromous fish, migrates from the Caspian Sea to rivers to have a more successful reproduction. The present study was aimed to evaluate the changes in the number and size of C. chalcoides gill chloride cells as well as to determine the relationship of its plasma prolactin with water salinity. Eighty-four individual C. chalcoides were collected from river (Lale Roud; 0.4 ppt), Lale Roud estuary (3.75 ppt), and Caspian Sea (9.71 ppt). The sampling was lasted for a-12 month period in 2014. The highest (1349±152) and lowest (881±37) number of gill chloride cells were observed in the animals collected from the Caspian Sea and in the river (Lale Roud), respectively. However, plasma prolactin demonstrated the highest level in C. chalcoides caught from the river (0.89±0.02 ng ml-1), but the lowest amount (0.70±0.03 ng ml-1) in the ones collected from the Caspian Sea. Overall, these results suggest that C. chalcoides migration across its habitats is an energy consuming ecological behavior, and the fish consumes high energy just after breeding and while returning to the Caspian Sea.   144 Fish processing Effect of different packaging on the shelf life of silver carp (Hypophthalmichthys molitrix) fillets stored at 4 ºC Rahmatipoor R i Roomiani L j Askary Sary A k i islamic azad u j islamic azad u k islamic azad u 1 11 2017 3 2 22 35 11 07 2017 13 08 2017 Freshness is one of the main quality attributes for processing, marketing and consumption of fish. Fish is increasingly becoming the favored food of people in many countries as it is rich in proteins. However, the disadvantage associated with broader consumption of fish products is their comparatively short shelf-life. Therefore, effective methods for extending shelf-life and improving quality of fresh silver carp fillets are necessary. The goal of this study was to evaluate the biochemical and microbial changes of silver carp fillets in different packaging including aerobic, vacuum and modified atmosphere packaging (MAP) when stored at 4ºC for 15 days. Data obtained from these tests was averaged and subjected to the analysis of variance (ANOVA) using SPSS software. The fillets packaged at MAP had significantly (p<0.05) lower pH, TVB-N, TBA and PV contents than those packaged at vacuum and aerobic packaging. The increase in viable bacterial population was significantly (p<0.05)higher in samples packed at vacuum and aerobic. The results showed MAP combined with refrigerated storage resulted in an extension of the shelf-life of fillets; up to 11 days at MAP, 12 days at vacuum compared to 3 days in aerobic samples.   133 Toxicology and polution Histopathological changes in various tissues of striped catfish, Pangasianodon hypophthalmus, fed on dietary nucleotides and exposed to water-borne silver nanoparticles or silver nitrate Pournori B l Paykan Heyrati F m Dorafshan S n l Department of Natural Resources Isfahan University of Technology Isfahan, 84156-83111, Iran m Department of Natural Resources Isfahan University of Technology Isfahan, 84156-83111, Iran n Department of Natural Resources Isfahan University of Technology Isfahan, 84156-83111, Iran 1 11 2017 3 2 36 52 09 04 2017 31 08 2017 The objective of this paper focused on the ffects of dietary nucleotides (NT) on histopathological alternations of striped catfish Pangasianodon hypophthalmus, after exposure to water-borne silver nanoparticles (AgNPs) and silver nitrate (AgNO3). Fish were fed with a diet containing nucleotide (0.75%) or control diet for 10 weeks and then divided into 3 experiments including control, 20 µg L-1 of AgNPs or AgNO3 for 10 days. At the end, histopathological changes in gill, liver and kidney were evaluated using haematoxylin-eosin technique. Water-borne AgNPs or AgNO3 caused some distinctive histopathological alterations in both feeding group. The most important damages were observed in epithelial cell hyperplasia, inflammation and necrotic epithelial cell in the gills, pigmentation, fiber cells and cytoplasmic vacuolization of hepatocytes in the liver and visualization of red blood cells and eosinophils, glomerular and tubular necrosis in the kidney. Based on organ index (Iorg), the highest damages were observed in the tissues of the fish fed on the control diet and exposed to 20 µg L-1 of AgNPs. No significant differences were observed in histopathological alterations between two feeding groups when compare the same organs with similar pollutant (kind and concentration). It could be concluded that dietary NT could not improve the fish ability against water-borne AgNPs or AgNO3.   142 Toxicology and polution Emerging contaminants in raw water supply in the east of Tehran Zarei M o Moogouei R p Tabari M o islamic azad u p islamic azad u islamic azad u 1 11 2017 3 2 53 60 17 06 2017 20 08 2017 Emerging contaminant in drinking water has created concerns among public, water supplier and government. Pharmaceutical in water and food even in low concentration have shown potential risks to human. Acetaminophen and ibuprofen are among a set of the most prescribed drugs in the world. In this study raw water and treated drinking water from Latyan drinking water treatment facilities were analyzed to determine possible concentration of acetaminophen and ibuprofen in water. All the data were subjected to analysis of variance to consider significance of differences. Furthermore mean comparison between data was calculated using Duncan test. Presence of acetaminophen in the ground water resources was not reported. While in the Jajrood river and Latyan Dam Lake the concentration of acetaminophen were 2.16 ± 0.09 and 9.17 ± 0.18  ηg L-1 respectively. In the case of ibuprofen the only observed concentration was equal to 11.49 ± 0.33 in the Latyan Dam Lake. No concentration of acetaminophen and ibuprofen was detected in drinking water samples gathered after treatment process.Why pharmaceuticals were known as indicators for controlling the quality of raw water and the treatment efficiency in the drinking water treatment facilities, ground water and treated water of this area are safe with no detection of acetaminophen and ibuprofen. 147 Treatment stategies with herbal or others Effect of Zataria multiflora Boiss on haematological and Growth parameters of Oncorhynchus mykiss Choobkar N Kermanshah Branch, Islamic Azad University 1 11 2017 3 2 61 73 11 08 2017 28 09 2017 Zataria multiflora is well known to middle-east, Iran, Afghanistan and Pakistan where, it is cultivated only in warm areas. The objective of this article focused on this issue whether Zataria multiflora Boiss has effect on haematological and Growth parameters of Oncorhynchus mykiss. One hundred and twenty healthy finger ling fish, Oncorhynchus mykiss (mean weight 4.3 g) were commercially obtained and then transferred to the research field located in Tehran. Fish were fed on diets covering 0, 50, 100 and 150 mg kg-1 Z. multiflora (Z.M.) as four groups; in triplicates. All of them were hand-fed (3-4% of body weight) to satiation 6-8 times a day for 8 weeks (60 days). There was no significant difference (p> 0.05) among the fish fed the different levels of Z.M for the mean weight. The maximum value of SGR was observed in group 50 ppm of Z.M. (2.85 ± 0.01) with significant difference (p< 0.05) with control (2.44 ± 0.0). Accordingly, this result showed that FCR value was minimum in control (7.20 ± 0.0) with no significant difference (p> 0.05) compared with group 50 ppm of Z.M. The result of WG for group 50 ppm of Z.M. (453.86 ± 0.46) showed a significant difference (p< 0.05) with those of other groups. On the other hand, Z.M. in concentration of 50 ppm could enhance WBC compared to control and other treatments as well as RBC but no difference was observed among the treatments (p> 0.05). Against, the values for Hb and MCH were significantly lower than others (p< 0.05). It is concluded that the fish fed with 50 ppm of Zataria multiflora increased mean weight of rainbow trout while enhancement of innate immunity significantly was occurred.   140 Toxicology and polution Evaluation of silver nanoparticles toxicity in Daphnia magna: Comparison of chemical and green biosynthetic productions Tulaby Dezfuly Z Alishahi M Aramoon A Mashjoor S Shahid Chamran University of Ahvaz Department of Clinical Sciences Faculty of veterinary, Shahid Chamran University of Ahvaz, Ahvaz, Iran Shahid Chamran University of Ahvaz, Ahvaz, Iran Department of Marine biology, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran 1 11 2017 3 2 74 85 07 06 2017 14 10 2017 Recently nanoparticles, particularly silver nanoparticles, are broadly used in industry, hence the contamination of the environment with AgNPs has caused considerable concern. In this study, the toxicity of biosynthetic nanosilver produced by two macroalgae: Sargassum boveanum and Ulva flexuosa extracts were compared with chemical nanosilver in Daphnia magna. Size and quality of nanoparticles evaluated by TEM, FT-IR spectrum, and Particle size analyzer. The acute toxicity test was evaluated following the OECD and Test guideline No: 211. D. magna were reproduced using parthenogenesis from a single individual according to OECD guideline. Then Daphnia exposed to eight serial dilutions of each nanosilver in triplicates for 48 hours. The mortality rate after 12h, 24h, 36h, and 48h were recorded and analyzed using probit software. Results showed that all nanosilver (regardless of their synthesis origin) were toxic in Daphnia and acute toxicity of this nanosilver was different (p<0.05).  The 48h LC50 of SPN, UPN, and CPN in Daphnia were 1.03, 3.24 and 0.03 mg L-1 respectively. The mortality rate in D. magna enhanced in all tested groups, along with increasing nanosilver concentration and exposure time duration. Highest toxicity belongs to chemical nanosilver (LC50 = 0.03 mg L-1), which was 30 and 100 times more toxic than SP (LC50 = 1.03) and UP (LC50 = 3.24 mg L-1) respectively. According to the high toxicity of chemosynthetic nanosilver compare to biosynthetic ones, biosynthetic nanoparticles are highly recommendable and environmentally friendly alternative to chemical oriented nanoparticles.   146 Aquaculture and Health management Effect of oral administration of Levamisole, Quil-A and Cinnamon in growth amount, hematological and immune parameters of Marmalade cichlid, Labeotrophus fuelleborni (Ahl, 1926) Amiri Z Bahrekazemi M Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran 1 11 2017 3 2 86 97 06 08 2017 24 10 2017 The study evaluated the effects of Levamisole, Quil-A and Cinnamon on growth, hematological and immune parameters of marmalade cichlid. Fish (2.00 ± 0.02 g) were fed diets supplemented with 0.2% Levamisole, 0.5% Quil-A and 1% Cinnamon for 60 days and the control group was fed with basal diet. The highest weight gain and condition factor were observed in fish fed diet enriched with Levamisole followed by Quil-A. There was no significant difference between the three treatments in specific growth rate (SGR) (p>0.05). However, the SGR showed a significant difference in the control group (2.16 ± 0.02% day-1) compared to other treatments (p<0.05). No significant difference in survival rate was observed between treatments (p>0.05). The lowest FCR was recorded in fish fed diet enriched with Levamisole (1.74 ± 0.95). The highest levels of RBCs, hemoglobin and hematocrit were observed in Levamisole treatment, but the highest number of WBCs was recorded in Cinnamon treatment (16766700 ± 450.92 mL-1). The maximum level of lysozyme was also observed in Cinnamon treatment (8.84 ± 0.38 µg mL-1). While no significant difference was recorded in the levels of the basophil, eosinophil, and monocyte, the highest levels of neutrophils (25.00 ± 1.0%) and lymphocytes (78.33 ± 0.57%) were observed in Cinnamon treatment. So, the use of Levamisole significantly improved growth performance and hematological parameters, but immunological parameters were improved with fish fed diet supplemented with Cinnamon. 148 Aquaculture and Health management Characterization of complement activity in serum of the four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae) Yektaseresht A Gholamhosseini A Janparvar A 1Department of Pathobiology, School of Veterinary Medicine, Shiraz Universi 2Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. 1Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. 1 11 2017 3 2 98 109 19 08 2017 14 11 2017 Serum complement is valuable tool in determining the health status of fish. This study was conducted to examine in vitro activity of serum complement as an indicator of innate immunity in four sturgeon species, Acipenser stellatus, A. baeri, A. nudiventris and Huso huso (Chondrostei: Acipenseridae). The Effects of different temperatures, concentrations and volumes of serum on alternative complement pathway activity were evaluated using standard haemolytic assays in four sturgeon species (A. stellatus, A. baeri, A. nudiventris and Huso huso). Results indicated significant relation between measured hemolysis and concentration, volume and temperature (p<0.05). Maximal hemolytic activity was exhibited at 100% serum in four sturgeon species. (p<0.05). Exposure of different volumes of serum from sturgeon species to RBCs exhibited volume dependent hemolysis (p<0.05). Maximal hemolytic activity was resulted in 30 μL of serum for all the sturgeon species. The highest hemolytic activity occurred at 4 °C for all the sturgeon species, but the hemolytic activity decreased at 37 °C. The results showed that the alternative complement pathway activity in sturgeon species serum may be affected by temperature, concentration and volume of serum that it caused an increase in non-specific immunity and the resistance to the outbreak of diseases.     158 Preventive and Control Strategies including engineering models Feasibility study providing a software model to predict incidence of White Spot Syndrome Virus in shrimp ponds contributed with physical and chemical factors in Iran Kakoolaki S Sepahdari A Mehrabi M Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Tehran, Iran. Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Tehran, Iran. Agricultural Research Education and Extension Organization (AREEO), Iranian Fisheries Science Research Institute (IFSRI), Tehran, Iran. 1 11 2017 3 2 110 117 27 09 2017 21 11 2017 The objective of this survey was to provide a software ecological model to predict the incidence of WSSV in Litopenaeus vannamei ponds of Gwater area located in southeastern of Iran. The coding and grouping of effective factors, which were involved in the occurrence of White Spot Syndrome Virus (WSSV) including temperature, salinity, oxygen, pH, ammonia, silica, pond preparation and management and water phytoplankton with the critical-to-optimal ranges had being applied.  The data were originated from a national research plan named epidemiological study of the effect of environmental and management factors affecting the incidence of WSSV of Fenneropenaeus indicus and L. vannamei. The type and quality of processes in the emergence of the effect of each exposure in the appearance of the outcome of WSSV were designed. In this design, meaningful effective factors directly contributed to the development of the process, and other factors merely assumed as complementary form of disease prognosis. It is concluded that the ecological model can shows the role of the physicochemical and management factors in incidence of WSSV in the area where the disease was already encountered with.