1 2821-1383 Iranian Association of Aquatic Animal Health 118 Treatment stategies with herbal or others Evaluationthe effects of different levels of Echinacea purpurea extract on the immunity responses, biochemical and hematological indicesand disease resistance against Streptococcus iniae in rainbow trout (Oncorhynchusmykiss) Sharif Rohani M b Pourgholam R c Haghighi M d b Iranian Fisheries Science Research Institute (IFSRI), Agriculture Research, Education and Extension Organization (AREEO), Tehran-Iran c ranian Fisheries Science Research Institute (IFSRI), Caspian Sea Ecology research Center, Agriculture Research, Education and Extension Organization (AREEO), Sary-Iran d Iranian Fisheries Science Research Institute (IFSRI), Coldwater Fishes Research Center (CFRC), Agriculture Research, Education and Extension Organization (AREEO), Tonekabon-Iran 1 11 2016 2 2 1 13 16 02 2017 16 02 2017 240 rainbow trout fingerlings weighting 16 gram were randomly allocated into three treatment groups including 0.5, 1.0and 1.5 gr/kg of Echinacea purpurea extract in food, and a control group (without EPE in food), each intriplicates for 60 days. At sampling times, some of hematological and biochemical parameters including C3 and C4 complements, oxygen free radicals, lysozyme activity, and other hemathological parameters were analyzed. Also, at the end of the experiment,fishes challenged with Streptococcus iniae and the mortality was analyzed. However, there were no significant differences in C4, total immunoglobulin, lymphocyte and monocyte (p>0.05). The amount of glucose, serum total protein and albumin decreased at the end of the experiment significantly (p<0.05). In addition, the results showed that mortality percentage in Echinacea treatment group with 1.5 gr/kg concentration was significantly lower than other treatment groups (p<0.05). In conclusion the results haveshown that Echinacea extract had the positive effect on immunity responses of rainbow trout especially in higher concentration (1.5gr/kg). Also, adding Echinacea extract in rainbow trout diet increased the resistance of rainbow trout against S. iniae.
119 Treatment stategies with herbal or others The efficacy of Echinacea (Echinacea purpurea) methanol extract on growth performance in grey mullet (Mugil cephalus) Akbary P e Kakoolaki S f Salehi H g Zorriehzahra m j h Sepahdari A i Mehrabi M R j Jadgal S k e Department of Marine Sciences, Chabahar Maritime University, Fisheries group, Chabahar, Iran f ranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran g ranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran h ranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran i ranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran j ranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran k Offshore Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Chabahar, Iran 1 11 2016 2 2 14 24 16 02 2017 16 02 2017 This experiment was conducted to evaluate the efficacy of different levels of Echinacea (Echinacea purpurea) methanol extract on the growth performances (final weight (FW), daily growth ratio (DGR), feed conversion rate (FCR), voluntary feed intake (VFI) and protein efficiency ratio (PER) and some of hematological parameters (hemoglobin (Hb), hematocrit (Hct), red blood cell (RBC) and white blood cell (WBC) of  grey mullet (Mugil cephalus). The experiment was conducted in a completely randomized design with 360 of larvae (with average weight of 0.75±0.03g) in 4 treatments: control group without using Echinacea extract, an another groups (treatment 2, 3 and 4) the amounts of this  extract were 50,100 and 200 g/kg food. The highest FW (4.22±0.11g), DGI (1.72±0.50%) and the lowest FCR (0.95±0.05) and VFI (1.77±0.05%), were observed in treatment 4. But treatment 4 in all of these growth parameters did not show a significant difference compared with treatment 3 (P> 0.05). After 60 days, treatments 3 and 4, showed significantly higher RBC, WBC, Hb and Hct than those fed the control diet. Finally, the present results suggest that diet containing 100 and 200 g kg-1 extract could improve growth and hematological parameter of M. cephalus. 120 Toxicology and polution Measurement the heavy metals content (Cd, Ni and Pb) in the muscle tissue of Psettodes erumei and Psettodes erumei of the Qeshm Island (Persian Gulf) Gholami S l Aghanajafizadeh S m Naderi M n l Department of Environment, Bandarabbas Branch, Islamic Azad University, Bandarabbas, Iran m Department of Environment, Maybod Branch, Islamic Azad University, Maybod, Iran n Department of Environmental Sciences, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran 1 11 2016 2 2 25 33 16 02 2017 16 02 2017 This study conducted to determine the amount of the heavy metals such as Cd, Ni, and Pb in the muscle tissue of Hoof benthic fish (Psettodes erumei) and urban pelagic fish () in Qeshm Island in the north of the Persian Gulf. Overall, the heavy metal content Lethrinus nebulosus of 30 tissue samples was randomly measured by atomic absorption spectrometer. The mean amount of Cd (0.16+ 0.002 ) and Ni( 0.56+ 0.03 ) in benthic Hoof was significantly greater than that in the Urban pelagic fish while the concentration of (29.64+ 3.72) in urban pelagic fish was higher than that in the Hoof tissue samples (P<0.05). Our results showed that the concentration of the three measured heavy metals in the fishes studied was less than the standard levels proposed by WHO and FAO 121 Parasitic Diseases Occurrence and histopathological effect of Ligula intestinalis on Sea bream (Abramis brama Orientalis) Bozorgnia A o Omidzahir Sh p Hoseini S M Darzi Sh o Faculty of Veterinary Medicine, Islamic Azad University, Ghaemshahr branch, Ghaemshahr, Iran p Faculty of Marine Sciences, University of Mazandaran,Babolsar, Iran Faculty of Veterinary Medicine, Islamic Azad University, Babol branch, Babol, Iran Faculty of Veterinary Medicine, Islamic Azad University, Ghaemshahr branch, Ghaemshahr, Iran 1 11 2016 2 2 34 43 16 02 2017 16 02 2017 Ligula intestinalis originally is belongs to Cestoda parasite that infests a range of fish family especially Cyprinidae. It is also a zoonosis parasite that affects fish and probably human health. This study tried to isolate L. intestinalis from Sea bream (Abramis brama Orientalis), an species classified in family, Cyprinidae, from Caspian Sea. Sixty Sea bream were caught from Caspian Sea, Babolsar Fisheries port. Among that, 26.66% were infected with L. intestinalis. The tissues of infected fishes including gonads, liver and kidney were examined for pathological findings. Ovaries changes showed fibrosis, infiltration of inflammatory cells, atrophy of oocytes; testicular hyperemia, necrosis, fibrosis and degeneration; kidneys necrosis, destruction of tubules, vacuolar degeneration; and liver vacuolar degeneration, biliary duct hyperplasia, necrosis, lymphatic vessel dilatation, fibrosis and cholestasis. Considering the effect of L. intestinalis on fish and human health, the prevention programs to disturb the cycle of parasite and more monitoring studies in fish epidemiology especially food fish are recommended. 122 Treatment stategies with herbal or others Effect of different concentration of profitable Bacillus on bioremediation of common carp (Cyprinus carpio) pond discharge Naderi Samani M Jafaryan H Gholipour H Harsij M Farhangi M 1 Ph.D student of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Fishery, Gonbad University of Agriculture Sciences and Natural Resources, Gonbad, Iran Department of Fishery, Gonbad University of Agriculture Sciences and Natural Resources, Gonbad, Iran Department of Fishery, Gonbad University of Agriculture Sciences and Natural Resources, Gonbad, Iran Department of Fishery, Gonbad University of Agriculture Sciences and Natural Resources, Gonbad, Iran 1 11 2016 2 2 44 54 17 02 2017 17 02 2017 A 4 days study was conducted to determine the effects of Different concentration of bioaugmentor bacterial strain Bacillus licheniformisi, B. subtilis, B. polymyxa, B. laterosporus and B. circulans (Protexin Aquatech, UK) on adjustment of parameters of water quality as TAN, NO2-N, NO3-N and turbidity from the effluent of Common Carp ponds. Effects of time and concentration were studied as a completely randomized split-plot design. Time and concentration and their interaction had a significant difference (P<0.05) on changes of total ammonium nitrogen, nitrate nitrogen, nitrite nitrogen and turbidity. The total counts of bacteria recorded in the water of bioremedated tanks were also lower than that in the control tank.  123 Aquaculture and Health management The Effects of Dietary Probiotic Pediococcus acidilactici on the Growth Performance and Survival rate of Oriental Bream Fry (Abramis brama orientalis) Asadi Khomami S Mooraki N Valipour A Kakoolaki S Young Researchers and Elites Club , North Tehran Branch, Islamic Azad University, Tehran, Iran Faculty of Fisheries, Islamic Azad University, North Tehran Branch, Tehran, Iran Aquaculture Institute of Inland Waters, Bandar Anzali, Guilan, Iran Aquatic animal Health and Diseases Department, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran 1 11 2016 2 2 55 66 17 02 2017 17 02 2017 The present study tried to investigate the effects of probiotic, Pediococcus acidilactici in concentrations of 1× 109, 2× 109 and 3×109cfu kg-1 diet on growth and survival rate of oriental bream fry (Abramis brama orientalis, Berg 1949) in comparison to control diet (devoid of probiotic) for 60 days. In so doing, 180 specimens of oriental bream with initial weight of 2.69± 0.22 g were divided randomly into12 fiberglass tanks (capacity 110 l).They were fed at 3-5 percent body weight daily. A the end of the trial, growth and survival rates were measured.  The best growth performance was viewed in treatments rather than the control because of the improvement of gut microflora balance, which performed by probiotic P. acidilactici, following by the enzyme secretion for increasing nutrient digestibility and fish appetite. The best bodyweight gain, specific and daily growth rates, and  the lowest feed conversion ratio was observed in treatment, feeding with diet containing 2× 109cfu kg-1 diet (P<0.05). The results of the current study revealed that it is the role of probiotic, P. acidilactici to the diet of oriental breamfry and A. brama orientalis, which resulted in growth performance increase and feed efficiency ratio improvement.  124 Vaccination Profile and protective role of 61kDa and 47kDa antigens of Edwardsiella tarda in rohu (Labeo rohita) Kasagala KH.D.T. Pani Prasad K Makesh M Gireesh Babu P Veterinary Research Institute, Sri Lanka Central Institute of Fisheries Education, Mumbai, India Central Brackishwater Research Institute, Chennai, India Central Brackishwater Research Institute, Chennai, India 1 11 2016 2 2 67 87 17 02 2017 17 02 2017 Edwardsiella tarda is a versatile pathogen that could survive in different environmental conditions and infect economically important fish species. The whole cell antigenic proteins were extracted from E. tarda and assessed via Western blot using anti E. trada rohu and anti E. trada rabbit serum. Two strong reacted proteins with antibodies were recovered viz 61 kDa and 47 kDa from SDS PAGE gels to evaluate their vaccine potential in rohu fish. Fish were vaccinated 10 µg of antigenic proteins intraperitoneally as immunogens with Freund’s incomplete adjuvant (FIA). The boosters were given on the 10th day of immunization with PBS. The blood was drawn from vena caudalis at every 7th day for hematological and immunological studies. Fish were challenged on 35th day with 1× 106 cfu E. tarda field isolate ET-1. The cumulative mortality (CM) was recorded over one week and relative percentage survival (RPS) was calculated. The 61 kDa and 47 kDaproteins had significantly higher RPS, white blood cell count, specific and nonspecific parameters over control group. 61 kDa protein gave significantly high NBT activity, lysozyme activity and specific antibodies for E. tarda. 47 kDa protein hadsignificantly high phagocytic index and myeloperoxidase activity. The phagocytic capacity was significantly high at 3rd week of 47 kDaprotein. 61 kDaand 47kDa proteins had RPS of 59% and 52% respectively after challenge studies. Non specific immune parameters were more active at the first four weeks of immunization and specific antibodies for E. tarda were significantly higher since second week and peaked at 4th week in this study. Therefore 61 kDa could be considered as best vaccine candidateover 47 kDa for E. tarda infection in rohu fish. 127 Treatment stategies with herbal or others The Effect of Gamma (γ) Irradiation to inactivate Escherichia coli in Contaminated water Ahari H Alinejad Dizaj M Paidari S Anvar A A Assistant Professor, Department of Food Science and Technology Science and Research Branch, Islamic Azad University, Tehran, Iran Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Food Science. Technology Science and Research Branch, Islamic Azad University, Tehran, Iran Assistant Professor, Department of Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran. 1 11 2016 2 2 88 96 18 02 2017 18 02 2017 Several common approaches are used for disinfection of water in this section, but using the approaches (e.g. chlorination) are accompanied with hazardous by-products and also lead to notable changes in odor and flavor of water. Gamma irradiation as an emerging method has been encouraged currently by FAO/WHO for water, wastewater and food treatment. Coliform bacteria belong to common contaminants of water and being used as an indicator of efficacy of water safety measures, including gamma irradiation. Current study was conducted to evaluate the efficacy of gamma irradiation to eradication of microbial contaminants from food industry water resources. Water samples were collected from food industries located around Tehran, capital of Iran. Samples from three major resources of food industry water system; Well, Tank and Recycled water were collected and one part of the samples irradiated with 137Cs gamma radiator. Treated and untreated samples were tested by standard Multiple-tubes fermentation technique for detection of coliform bacteria and MPN indexes were calculated. Gamma irradiation had killing effect on coliforms and leads to noticeable reduction in MPN indexes of treated water samples compared to untreated ones. With few modifications in radiation dose, it is expected to implant portable gamma radiators to treatment of water in food industry section. 128 Treatment stategies with herbal or others Effects of antifungal activity of Daenensis thyme (Thymus daenensis)and Mentha (Mentha longifolia)essential oils on rainbow trout(Oncorhynchus mykiss) eggs hatchability Salehi M Soltani M Hosseini-Shekarabi S. P. Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. 1 11 2016 2 2 97 107 21 02 2017 21 02 2017 In this study, rainbow trout (Oncorhynchus mykiss) eggs were treated with effective doses of  Daenensis thyme, Thymus daenensis (20, 10 and 5 mgL-1) and Mentha, Mentha longifolia (10, 5 and 2.5 mgL-1), during incubation period (20 days) until hatching for 30 minutes per every other day as constant flow bath treatment method. The mortality level in normal control (without any treatment) was significantly higher than other groups (p<0.05). Level of mortality in egg treated with Daenensis thyme at 20 mgL-1(12.01±0.357%) was significantly lower than both normal control (33.35±0.889%) and Mentha groups until the eyed-egg stage (p<0.05). No significant different was seen between Mentha at 10 and 5 mgL-1mortality untileyed-egg stage(p>0.05).  The highest hatching rate (78.36±0.340%)was recorded in Daenensis thyme at 20 mgL-1concentrations compared to other groups except Malachite green (p<0.05). Eggs treated with Daenensis thyme and Mentha essence showed greater mean percentage of survival and hatching rates compared to the normal control (p<0.05). However, essential oil derived from Daenensis thyme at 20 mgL-1 probably has the potential to be used as health control of rainbow trout eggs against fungal contamination.