Research Article: Morphometric study and identification of external parasites in Hypophthalmichthys molitrix breeders in Guilan, Iran 2 2 The parasites that normally cause fish disease under farming condition possess a direct life cycle with no need to intermediate hosts for dissemination. The ensuing infection can therefore spread rapidly inducing more damaging lesions in the external parts of host especially the gills. In this study, in order to isolate and identify foreign parasites of phytophagous fish in Guilan province, 4 farms were selected by cluster method from Silver Carp breeding farms around Rasht and 16 pieces of fish in 6 stages for 6 months from April to September 2020. The fish transported live to fish health lab, underwent individual clinical observation of their skin, fins and gills with at least 10 moist extended samples prepared for each part. Isolation and morphometric identification of samples were carried out via microscopic examinations. The collected data were statistically analysed using Excel version 2010 and SPSS 20 software. All the diagrams and table contents representing the intended parameters including frequency and the means were processed by Chi-square critical value of percentage 5. The results of the study showed a similar infestation outbreak of L. Trichodina on the fish fins and gills with no significant difference (P>0.05). Dactyl gyrus spp was more prevalent in fish gills and fins than on skin (P<0.05) but the prevalence rate of Dactylogyrus spp, chilodonellosis, cryptobia and Ichthyophthirius on the gills of phytophagous breeders were more than the rest of examined areas showing a significant difference with that of skin and gills (P<0.05). 1 9 2021/05/2 1400/2/12 2021/07/23 1400/5/1 M Rahanandeh M Rahanandeh Guilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran Iran Rahanandeh1340@gmail.com B Tizkar B Tizkar Guilan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran Iran btizkar@areeo.ac.ir Hypophthalmichthys molitrix Fish breeders external parasites Guilan Abdul-Ameer, K.N., 2004. The first record of the ciliated protozoan Trichodina cottidarum in Iraq on the gills of the common carp (Cyprinus carpio). Ibn Al-Haitham Journal For Pure And Applied Science, 17(3), 1– 6.##Aksoy, S., Saglam, N. and Dorucu, M., 2006. External parasites of three cyprinid fish species from Lake Hazar in Turkey. Indian veterinary journal, 83(1), 100-101.##Bhuiyan, A.S., Akther, S. and Musa, G.M., 2007. Occurrence of parasites in Labeo rohita (Hamilton) from Rajshahi. University journal of Zoology, Rajshahi University, 26, 31-34. https://doi.org/10.3329/ujzru.v26i0.694.##Blazhekovikj, D.D. and Stojanovski, S., 2015. Silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis) as hosts of Sinergasilus polycolpus, a new copepod species in the ichthyoparasitofauna of Macedonia. Food Science and Quality Management, 45, 32-38.##Chandra, K.J., 2004. Fish Parasitology. Lima Printing Press, Mymensingh, p. 176. ##Christoffersen, T.B., Kania, P.W., von Gersdorff Jørgensen, L. and Buchmann, K., 2017. Zebrafish Danio rerio as a model to study the immune response against infection with Ichthyophthirius multifiliis. Journal of fish diseases, 40(6), 847-852. https://doi.org/10.1111/jfd.12543.##Ghorbanzadeh, R. and Nazari, S., (2013) Ian Fisheries Organization, Statistical Yearbook, pp. 28. (In Persian).##Gussev, A.V., 1985. Parasitic metazoans: Class Monogenea. In: Bauer, O.N. (Ed.) Key to the parasites of freshwater fish fauna of the U.S.S.R. Nauka, Leningrad, 2, 1-424. ##Hussain, H.T., 2005. Ectoparasitic infection of the common carp and silver carp Fingerlings stocked during winter in AL-Shark AL-Awsat fish farm, Babylon Province, Iraq. M.Sc. thesis. Coll. AL-Mussab Technical Foundation of Technical Eduction: pp.106.##Jalali, B. and Barzegar, M., 2006. M. Fish parasites in Zarivar Lake. Journal of Agricultural Science and Technology, 8, 47-58.##Jalali, B., 1998. Parasites and parasitic diseases of Iranian freshwater fish, Iranian Department of Aquaculture, Iran Fisheries Publications, Tehran, Iran 1 Ed.p. 155-167. (In Persian).##Jeney, Z. and Jeney, G., 1995. Recent achievements in studies on diseases of common carp (Cyprinus carpio L.). Aquaculture, 129, 397–420. https://doi.org/10.1016/0044-8486(94)00283-T.##Jha, D.K. and Bhujel, R.C., 2012. Incidence of fish diseases and management practices in Nepal. Small-scale Aquaculture for Rural Livelihoods, 160-165.##Karunasagar, I., Karunasagar, I. and Otta, S.K., 2003. Disease problems affecting fish in tropical environments. Journal of Applied Aquaculture, 13(3-4), 231-249. https://doi.org/10.1300/J028v13n03_03.##Klinger, R. and Francisbyd, R., 2005. Introduction to fresh water fish parasites. University of Florida, P. 162-163. ##Malmberg, G., Collins, C., Cunnigham, C. and Jalali, B., 2007. Gyrodactylus derjavinoides sp.nov. (Monogenea, Platyhelminthes) on salmo trutta trutta L. and G. Derjavini Mikailov, 1975 on s.t. caspius Kessler, two different species of Gyrodactylus- Combined morphological and molecular investigations. Acta parasitology, 52(2), 89-103. https://doi.org/10.2478/s11686-007-0016-1.##Molnar, K., 1994. Effect of decreased water oxygen content on common carp fry withm D. vastator (monogenea) infection of varing severity. Diseases of Aquatic Organism, 20, 153-157. https://doi.org/10.3354/dao020153.##Muhammed, S.K., 2000. An external and eye parasite survey for carp fishes in ALEskandaryia Region (Babylon). Ph.D. Thesis Coll. Vet: Med., Univ. Baghdad, P. 82.##Nematollahi, A., Ahmadi, A., Mohammadpour, H. and Ebrahimi, M., 2013. External parasite infection of common carp (Cyprinus carpio) and big head (Hypophthalmichthys nobilis) in fish farms of Mashhad, northeast of Iran. Journal of parasitic diseases. 37(1), 131-133. https://doi.org/10.1007/s12639-012-0146-8.##Noga, E.J, 2010. Fish disease:diagnosis and treatment, Two.Edi. 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(In Persian).##Rahanandeh, M., Sharifpour, I., Jalali, B., Kazemi, R., Aminian, B. and Shafiei, S. 2010. Survey on Dactylogyrus in Caspian Frisian roach (Rutilus frisii kutum) caused by Dactylogyrus frisii. Global veterinaria, 4, 515-518.##Raissy, M., Ansari, M. Lashkari A., and Jalali, B. 2010. Occurrence of parasites in selected fish species in Gandoman Lagoon. Iranian Journal of Fisheries Sciences, 9 (3), 464-471. ##Tekin-Özan, S., Kir, İ. and Barlas, M., 2008. Helminth parasites of common carp (Cyprinus carpio L., 1758) in Beyşehir Lake and population dynamics related to month and host size. Turkish Journal of Fisheries and Aquatic Sciences, 8(2), 201-205. https://doi.org/10.1007/s10661-007-9765-4 .##Woo, P.T.K., 2006. Fish Diseoses and Disorders, Volumel, Protozoan and Metozoam Parasites, CABinternational, Canada. 2Ed. P. 116- 124. https://doi.org/10.1079/9780851990156.0000.## ##

Research Article: Comparative histomorphology of epidermis of head and caudal peduncle in Otolithes ruber, Huso huso and Pangasius hypophthalmus fish 2 2 Three species of fish including, macroscopic scaled fish as Otolithes ruber, microscopic scaled sturgeon as Huso huso and free scaled cat fish as Pangasius hypophthalmus were prepared and specimen of dorsal of head and caudal peduncle were carried out. Routine procedures of tissues preparation followed and paraffin sections stained with (H&E) and (PAS). Results showed, epidermis formed non keratinized stratified squamous epithelium with epidermis, lymphocytes, goblet cells, taste bud and club cells. The epidermis thickness of the head skin was higher than that of the caudal peduncle, as demonstrated by image analysis using light microscopy. Goblet cells were along the superficial cells layers and their distributions were varied. In histomorphometric studies by PAS staining the highest number of these cells were seen in head of O. ruber and the lowest were seen in the caudal peduncle of P. hypophthalmus. Most of them were seen from the middle to surface layer of the epidermis. Club cells, with large nucleus, mostly evident in the deep and middle layer of the epidermis, being the largest cells within the epithelium. The highest numbers of these cells (61.8 ± 2.16) were found in head region of P. hypophthalmus. Taste buds as a sensory organ were not seen in caudal peduncle of O. ruber and H. huso. Based on the results of this study, epidermis had similarities in cell type and differences in their numbers that could be justified by the presence or absence of scales. 10 20 2021/05/22021/03/26 1400/1/6 2021/07/232021/08/11 1400/5/20 M Mohamed M Mohamed Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Iran Mohamed@yahoo.com R Abdi R Abdi Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Iran abdir351@gmail.com M. T Ronagh M. T Ronagh Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Iran Ronagh@yahoo.com M. A Salari - Ali Abadi M. A Salari - Ali Abadi Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran Iran Salari@gmail.com Z Basir Z Basir Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Iran z.basir@scu.ac.ir Histomorphology Skin Otolithes ruber Huso huso Pangasius hypophthalmus Abdi, R., Sheibani, M.T., Adibmoradi, M. and Sharifpour, I., 2007. Histological study of liver and pancreas in adult Otolithes ruber in Bushehr, Iran. Iranian Scientific Fisheries Journal, 15(4), 87-96. (In Persian)## Al-Banaw, A., Kenngott, R., Al-Hassan, J.M., Mehana, N. and Sinowatz, F., 2009. Histochemical analysis of glycoconjugates in the skin of a catfish (Arius Tenuispinis, Day). Anatomy Histology Embryology, 39, 42–50. https://doi.org/10.1111/j.1439-0264.2009.00977.x##Amiripour, L., Abdi, R., Movahedinia, A. and Sahraian, M.R., 2015. Study of Liver and Intestine Tissue Structure in Orange Spotted Grouper (Epinephelus coioides) During Larval Development. Journal of Oceanography, 6(23), 87-92. ##Barbosa, A., Magalhaes, E.J. and Hoffmann, A., 2010. Conspecific and heterospecific alarm substance induces behavioral responses in piau fish Leporinus piau. Acta Ichthyology, 13, 119–126. https://doi.org/10.1007/s10211-010-0081-6##Basir, Z. and Peyghan, R., 2019. Immunohistochemical and ultrastructural study of the effect of different salinities on gill chloride cells of Cyprinus carpio. Iranian Journal of Fisheries Science, 28 (5), 131-141.##Basir, Z. and Abdi, R., 2016. Histological study of WBC and hematological indices of spotted catshark Chiloscyllium punctatum in Persian Gulf during the cold season. Journal of Marine Science and Technology, 14(4), 15-21.##Basir, Z., Hassan, M., Mahabadi, M.K., Mesbah, M. and Abdi, R., 2015. Histomorphometric and Histochemistry of Mucous Secreting Cells in Different Parts of Skin in Shabut (Barbusgrypus, Heckel 1843). Journal of Applied Environmental and Biological Sciences, 5(10S), 80-85.##Ceballos-Francisco, D., Cordero, H., Guardiola, F.A., Cuest, A. and Esteban, M.A., 2017. Healing and mucosal immunity in the skin of experimentally wounded gilthead seabream (Sparus aurata L). Fish & shellfish immunology, 71, 210–219. https://doi.org/10.1016/j.fsi.2017.10.017##Cordero, H., Brinchmann, M.F., Cuesta, A., Meseguer, J. and Esteban, M.A., 2015. Skin mucus proteome map of European sea bass (Dicentrarchus labrax). Proteomics, 15, 4007–4020. https://doi.org/10.1002/pmic.201500120##Cordero, H., Ceballos-Francisco, D., Cuesta, A. and Esteban, M.A., 2017. Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata). PLoS ONE, 12(6), e0180438. https://doi.org/10.1371/journal.pone.0180438 ##Guardiola, F.A., Cuesta, A., Abellan, E., Meseguer, J. and Esteban M.A., 2014. Comparative analysis of the humoral immunity of skin mucus from several marine teleost fish. Fish & shellfish immunology, 40, 24–31. https://doi.org/10.1016/j.fsi.2014.06.018##Guardiola, F.A., Cuesta, A., Arizcun, M., Meseguer, J. and Esteban, M.A., 2014. Comparative skin mucus and serum humoral defence mechanisms in the teleost gilthead seabream (Sparus aurata). Fish & shellfish immunology, 36, 545–551. https://doi.org/10.1016/j.fsi.2014.01.001##Halbgewachs, C.F., Marchan, T.A., Kusch, R.C., Chivers, D.P., 2009. Epidermal club cells and the innate immune system of minnows. Biological Journal of the Linnean Society, 98, 891–897. https://doi.org/10.1111/j.1095-8312.2009.01328.x##Jensen, L.B., 2015. Effect of temperature and diet on wound healing in Atlantic salmon (Salmo salar L.). Fish Physiology and Biochemistry, 41, 1527–1543. https://doi.org/10.1007/s10695-015-0105-2##Jung, J.A. and Tonn, W.M., 2011. Alarm substances elicit limited population - level responses in fathead minnow. Ecology of Freshwater Fish, 20, 220–230. https://doi.org/10.1111/j.1600-0633.2010.00481.x##Karlsen, C., Ytteborg, E., Timmerhaus, G., 2018. Atlantic salmon skin barrier functions gradually enhance after seawater transfer. Scientific Reports, 8, 95-101. https://doi.org/10.1038/s41598-018-27818-y##Kim, C.H., Park, M.K., Kang, E.J., 2008. Minute tuber- cles on the skin surface of larvae in the Korean endemic bit- terling, Rhodeus Pseudosericeus. Journak of Applied Ichthyology, 24, 269–275. https://doi.org/10.1111/j.1439-0426.2007.01030.x##Lei, F.Z., Jiang, J.P., Li, C., Xie, F., 2012. Histological observation of skinfrom three species of Megophryinae. Chinese Journal of Zoology, 47(3),20-7, 2012.##Li, X.J., Peng, X.L. and Qiao, Z.G., 2010. Studies on the types,distribution andsecretion of mucous cells in the skin and gill of Silurus asotu. Journal of Shanghai Ocean University, 19(6), 751-5.##Lin, X., Zhang, W.N., Lin, S.G., Jiang, D.P. and Wang, S.K., 2008. Type anddistribution of mucous cells in skin, gills and digestive tracts of Anguilla anguilla. Fujian Journal of Agricultural Sciences, 1, 39-43.##Liu, Y., Xiao, Q., Yang, S., Zhao, L., Fu, H., Du, J., Du, Z., Yan, T. and Wu, H., 2017. Characterization of hematopoiesis in Dabry's sturgeon (Acipenser dabryanus). Aquaculture and Fishery Sciences, 2(6), 262-8. https://doi.org/10.1016/j.aaf.2017.10.007##Moradkhani, A., Abdi, R., Salari-Ali Abadi, M.A., Nabavi, S. and Basir, Z., 2020. Quantification and description of gut-associated lymphoid tissue in, shabbout, Arabibarbus grypus (actinopterygii: cypriniformes: cyprinidae), in warm and cold season. Acta Ichthyologica et Piscatoria, 50(4), 423-432. https://doi.org/10.3750/AIEP/02910##Naderi, S., Abdi, R., Navabi, M.B., Movahedinia, A., 2014. Distribution Pattern of Main Mucus Secretory Cells in Different Parts of Epiderm in Epinephelus coioides. International Journal of Scientific Engineering and Technology, 3(5), 630-633.##Park, J.Y., Oh, M.K, Kang, E.J., Kim, C.H. and Beon, M.S., 2010. On the vascularization and structure of the skin of a Korean bullhead Pseudobagrus brevicorpus (Bagridae, Teleostei) based on its entire body and appendages. Journal of Applied Ichthyology, 26, 64–70. https://doi.org/10.1111/j.1439-0426.2009.01354.x##Raj, V.S., Fournier, G., Rakus, K., Ronsmans, M., Ouyang, P., Michel, B., Delforges, C., Costes, B., Farnir, F., Leroy, B., Wattiez, R., Melard, C., Mast, J., Lieffrig, F. and Vanderplasschen, A., 2011. Skin mucus of Cyprinus carpio inhibits cyprinid herpesvirus 3 binding to epidermal cells. 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Research Article: Effects of pH-induced technique on flocculation efficiency and fatty acids profile of marine microalgae, Thalassiosira sp 2 2 Microalgae contain many valuable biological compounds, so their commercial production is of great importance. One of the important challenges in microalga production is their high cost associated with their harvesting processes. Therefore, finding efficient and cost-effective technologies for algae biomass separation is essential for achieving an operational process. The study was conducted to find the influence of pH variation from 4 to 11 on harvesting efficiency and fatty acids component of marine microalgae Thalassiosira sp under laboratory condition. The NaOH and HCl were added to adjust pH value in each treatment. Data was presented as mean ± SD. Comparison between treatments was performed using one-way analysis of variance ANOVA. The results showed that with increasing the pH up to 10, flocculation processes (92%) and concentration factor (20) increased significantly. While, lowering the pH from 8.2 to 4 was not effective in isolating algal biomass. In the next stage, the biomass fatty acids content was measured by both alkaline pH induction method and the centrifuge method. Analyzes showed that the percentage of lipid and saturated and unsaturated fatty acids in biomass collected by alkaline pH induction technique was lower than the centrifugation method. In general, the results of the present study showed that clotting in alkaline pH up to 10 can be suggested as a suitable method for harvesting of Thalassiosira sp microalgae, and if the production of polyunsaturated acids is considered, of consuming high amounts of sodium hydroxide should be avoided. 21 31 2021/05/22021/03/262021/06/10 1400/3/20 2021/07/232021/08/112021/08/11 1400/5/20 M Hobbi M Hobbi Department of Fisheries, Faculty of Marine Sciences, Fisheries Group, Chabahar Maritime University, Chabahar, Iran Iran pariaakbary1355@gmail.com P Akbary P Akbary Department of Fisheries, Faculty of Marine Sciences, Fisheries Group, Chabahar Maritime University, Chabahar, Iran Iran paria.akbary@gmail.com Z Aminikhoei Z Aminikhoei Offshore Fisheries Research Center, Iranian Fisheries Science Research Institute (IFSRI), Agricultural Research Education and Extension Organization (AREEO), Chabahar, Iran Iran paria.akbary@gmail.com Microalgae Thalassiosira sp pH-induced Harvesting efficiency Fatty acids Barros, , A. I., Gonçalves, A. L., Simões, M. and Pires, J. C. 2015., Harvesting techniques applied to microalgae: a review. Renewable and Sustainable Energy Reviews, 41, 1489-1500. https://doi.org/10.1016/j.rser.2014.09.037.##Becker, W., 2004. 18 Microalgae in Human and Animal Nutrition. Handbook of microalgal culture: biotechnology and applied phycology, 312- 351. https://doi.org/10.1002/9780470995280.ch18.##Besson, A. and Guiraud, P., 2013. High-pH-induced flocculation–flotation of the hypersaline microalga Dunaliella salina. Bioresource technology, 147, 464-470. https://doi.org/10.1016/j.biortech.2013.08.053.##Borowitzka,, M. A. and Borowitzka, L. J., 1988. Micro-algal biotechnology, Cambridge University Press.##Garcia, N., Lŏpez-ELias, J. A., Miranda, A., Martinez-Pprrchas, M., Hureta, N. and Garcia, A., 2012. Effect of salinity on growth and chemical composition of the diatom Thalassiosira weissflogii at three culture phases. Latin American Journal of Aquatic Research, 40, 435-440. http://doi.org/10.3856/vol40-issue2-fulltext-18.##Guillard, R. R., 1975. Culture of phytoplankton for feeding marine invertebrates. Culture of marine invertebrate animals. Springer. https://doi.org/10.1007/978-1-4615-8714-9_3.##Horiuchi, J.-I., Ohba, I., Tada, K., Kobayashi, M., Kanno, T. and Kishimoto, M., 2003. Effective cell harvesting of the halotolerant microalga Dunaliella tertiolecta with pH control. Journal of bioscience and bioengineering, 95, 412-415. https://doi.org/10.1016/S1389-1723(03)80078-6.##Iso, U., 2011. 12966-2 Animal and Vegetables Fat and Oils. Gas Chromatography of Fatty Acid Methyl Esters. Part 2: Preparation of Methyl Esters of Fatty Acids. International Organization for Standardization: Geneva, Switzerland.##KRISHNAKUMAR, S., BAI, V. D. M. & RAJAN, R. A. 2013. Evaluation of bioactive metabolites from halophilic microalgae Dunaliella salina by GC–MS analysis. Int. J. Pharm. Pharm. Sci, 5, 296-303.##Kwon, H., Lu, M., Lee, E. Y. and Lee, J., 2014. Harvesting of microalgae using flocculation combined with dissolved air flotation. Biotechnology and bioprocess engineering, 19, 143-149. https://doi.org/10.1007/s12257-013-0433-y.##Lang, Y., Del Monte, F., Rodriguez, B. J., Dockery, P., Finn, D. P. and Pandit, A., 2013. Integration of TiO 2 into the diatom Thalassiosira weissflogii during frustule synthesis. Scientific reports, 3, 3205-3216. http://doi.org/ 10.1038/srep03205.##Liu, J., Zhu, Y., Tao, Y., Zhang, Y., Li, A., Li, T., Sang, M. and Zhang, C., 2013. Freshwater microalgae harvested via flocculation induced by pH decrease. Biotechnology for biofuels, 6, 98-109. http://doi.org. 10.1186/1754-6834-6-98.##Maji, G., Choudhury, S., Hamid, S., Prashanth, R. and Sibi, G., 2018. Microalgae harvesting via flocculation: impact of pH, algae species and biomass concentration. Methods of Microbiology and Molecular Biology, 1, 1-5. ##Milledge, J. J. and Heaven, S., 2013. A review of the harvesting of micro-algae for biofuel production. Reviews in Environmental Science and Bio/Technology, 12, 165-178. https://doi.org/10.1007/s11157-012-9301-z.##Mixson, S. M., Stikeleather, L. F., Simmons, O. D., Wilson, C. W. and Burkholder, J. M., 2014. pH-induced flocculation, indirect electrocoagulation, and hollow fiber filtration techniques for harvesting the saltwater microalga Dunaliella. Journal of applied phycology, 26, 1701-1709.http://doi.org/ 10.1007/s10811-013-0232-z.##Pėrez, L., Salgueiro, J. L., Maceiras, R., Cancela, Á. and Sānchez, Á., 2017. An effective method for harvesting of marine microalgae: pH induced flocculation. Biomass and Bioenergy, 97, 20-26. https://doi.org/10.1016/j.biombioe.2016.12.010.##Sales, R. and Abreu, P. C., 2015. Use of natural pH variation to increase the flocculation of the marine microalgae Nannochloropsis oculata. Applied biochemistry and biotechnology, 175, 2012-2019.http://doi.org/ 10.1007/s12010-014-1412-2.##Show, K.Y., Lee, D. J. and Chang, J. S., 2013. Algal biomass dehydration. Bioresource technology, 135, 720-729. https://doi.org/10.1016/j.biortech.2012.08.021.##Spilling, K., Seppālā, J. and Tamminen, T., 2011. Inducing autoflocculation in the diatom Phaeodactylum tricornutum through CO 2 regulation. Journal of applied phycology, 23, 959-966.http://doi.org/ 10.1007/s10811-010-9616-5.##Uduman, N., Qi, Y., Danqah, M. K., Forde, G. M. and Hoadley, A., 2010. Dewatering of microalgal cultures: a major bottleneck to algae-based fuels. Journal of renewable and sustainable energy, 2, 012701. https://doi.org/10.1063/1.3294480.##Wan, C., Alam, M. A., Zhao, X. Q., Zhang, X. Y., Guo, S. L., Ho, S. H., Chang, J. S. and Bai, F. W., 2015. Current progress and future prospect of microalgal biomass harvest using various flocculation technologies. Bioresource technology, 184, 251-257. http://doi.org/ 10.1016/j.biortech.2014.11.081.##Wu, Z., Zhu, Y., Huang, W., Zhang, C., Li, T., Zhang, Y. and Li, A., 2012. Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium. Bioresource technology, 110, 496-502. http://doi.org/ 10.1016/j.biortech.2012.01.101.##Yang, F., Xiang, W., Fan, J., Wu, H., Li, T. and Long, L., 2016. High pH-induced flocculation of marine Chlorella sp. for biofuel production. Journal of applied phycology, 28, 747-756. http://doi.org/ 10.1007/s10811-015-0576-7.## ##

Research Article: Chlorophyta algae of Keban Dam Lake Gülüşkür region with aquaculture criteria in Elazıg, Turkey 2 2 Algae can be used as indicator for water quality, which could be a benefit for fish health. In this study, algae and water samples were taken from three selected stations from Keban Dam Lake Gülüşkür Region were investigated for six months between March 2015 and August 2015. A total of 48 taxa belonging to Chlorophyta were recorded during the study. Members of Chlorophyta showed their best growth in July and August, when light and temperature increased. Species belonging to Scenedesmus, Pediastrum, Ankistrodesmus and Oocystis genera were the most important members of phytoplankton with their frequency of occurrence and the size of the populations they formed. 32 46 2021/05/22021/03/262021/06/102021/05/10 1400/2/20 2021/07/232021/08/112021/08/112021/08/14 1400/5/23 G Pala G Pala Department of Fundamental Science, Faculty of Fisheries, Firat University, Elazig, Turkey Turkey M Caglar M Caglar Department of Fundamental Science, Faculty of Fisheries, Firat University, Elazig, Turkey Turkey mcaglar@firat.edu.tr R FARUQ R FARUQ Department of Fundamental Science, Faculty of Fisheries, Firat University, Elazig, Turkey Turkey Z Selamoglu Z Selamoglu Department of Medical Biology, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey Turkey zselamoglu@ohu.edu.tr Phytoplankton Chlorophyta Aquaculture Keban Dam Lake Elazığ Turkey Akbay, N., 1993. Keban Baraj gölü'nün ova kısmında fito ve zooplanktonun horizontal ve vertikal dağılımı. F.Ü. Fen Bilimleri Enstitüsü, Y.L. Tezi, Elazığ, Turkey.##Atıcı, T. and Obalı, O., 2005. Control of Water Pollution and Phytoplanktonic Algal Flora in Bayındır Dam Reservoir (Ankara). E.U. Journal of Fisheries & Aquatic Sciences, 22 (1-2), 79-82.##Atıcı, T. and Obalı, O., 2006. Seasonal variation of phytoplankton and value of chlorophylla in the Sarıyar Dam Reservoir. Turk J Bot, 30, 349-357.##Atıcı, T., 2004. Sarıyar Barajı Planktonik Algleri Kısım: I - Cyanophyta, Eğirdir Su Ürünleri Fakültesi Dergisi, II (XII), 88-98.##Aykulu, G. and Obalı, O., 1981. Phytoplankton Biyomas in the Kurtboğazı Dam Lake. Commun. Fac. Sci. Univ. Ank., Ser. C, 24, 29-44.##Baykal, T. and Açıkgöz, İ., 2004. Hirfanlı Baraj Gölü algleri. Gazi Üniversitesi KırşehirEğitim Fakültesi, 5 (2), 115-136.##Baykal, T. and Yıldız K., 2006. Çamlıdere Baraj Gölü Bacillariophyta dışı algleri. İstanbul Üniversitesi Su Ürünleri Dergisi, 20, 63-77.##Baykal, T., Açıkgöz, İ., Yıldız, K. and Bekleyen, A., 2004. A study on algae in Devegeçidi Dam Lake. Turkish Journal of Botany, 28 (5), 457-472.##Baykal, T., Salman, S. and Açıkgöz, İ., 2006. The relationship between seasonal variation inphytoplankton and zooplankton densities in Hirfanlı Dam Lake (Kırşehir, Turkey). Turkish Journal of Biology, 30 (4), 217-226.##Baytut, Ö., Gönülol, A., Arslan, N. and Ersanlı, E., 2006. The Phytoplankton of Karaboğaz Lake in Samsun, Turkey. Journal of Freshwater Ecology, 21(2), 359 - 361.##Cirik, S. and Cirik, Ş., 1995. Limnoloji. Ege Üniversitesi Su Ürünleri Fakültesi Yayınları No: 21, 166 p. Ege Üniversitesi Basımevi Bornova-İzmir, Turkey.##Çetin, A.K. and Şen, B., 1997. Keban Baraj Gölü'nün Bacillariophyta dışındaki algleri ve mevsimsel değişimleri. F. Ü. Fen ve Mühendislik Bilimleri Dergisi, 9(2), 45-49.##Ersanlı, E., 2001. Simenit Gölü (Terme-Samsun-Türkiye) algleri üzerine bir araştırma. Yüksek Lisans Tezi, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Samsun, Turkey.##Ersanlı, E., 2006. Çakmak Barajı (Tekkeköy-Samsun) fitoplanktonu ve mevsimseldeğişimi üzerinde bir araştırma. Doktora Tezi, Ondokuz Mayıs Üniversitesi FenBilimleri Enstitüsü, Samsun, Turkey.##Ersoy, H. N., 1996. Sinop İli bektaşağa ve taşmanlı göletleri alg florası üzerine biraraştırma. Yüksek Lisans Tezi, Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Samsun, Turkey.##Fogg, G. E. and Thake, B., 1987. Algal cultures and phytoplankton ecology, 3rdEdition, 269 p. The University of Wisconsin Press.##Gönülol, A. and Aykulu, G., 1984. Çubuk-I Baraj Gölü algleri üzerinde araştırmalar I. fitoplanktonun kompozisyonu ve yoğunluğunun mevsimsel değişimi. Doğa Bilim Dergisi, Az. 8(3), 330-342.##Gönülol, A. and Çomak, Ö., 1992a. Bafra Balık Gölleri (Balık Gölü, Uzun Göl) Fitoplanktonu üzerinde floristik araştırmalar I- Cyanophyta. Doğa, Turkish Journal of Botany, 16, 223-245.##Gönülol, A. and Çomak, Ö., 1992b. Bafra Balık Gölleri (Balık Gölü, Uzun Göl) Fitoplanktonu üzerinde floristik araştırmalar IV- Bacillorophyta, Dinophyta, Xanhtophyta. Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Fen Dergisi, 4(1), 1-19.##Gönülol, A. and Çomak, Ö., 1993. Bafra Balık Gölleri (Balık Gölü, Uzun Göl) Fitoplanktonu üzerinde floristik araştırmalar II. Euglenophyta. Doğa, Turkish Journal of Botany, 17, 163-169.##Gönülol, A. and Obalı, O., 1998a. A study on the phytoplankton of Hasan Uğurlu Dam Lake (Samsun-Turkey). Turkish Journal of Biology, 22, 447-461.##Gürbüz, H. and Altuner, Z., 2000. Palandöken (Tekederesi) Göleti fitoplankton topluluğu üzerinde üzerinde kalitatif ve kantitatif bir araştırma. Turkish Journal of Biology, 24, 13-30.##Gürbüz, H. and Kıvrak, E., 2003. Seasonal variations of benthic algae of Kuzgun Dam Reservoir and their relationship to environmental factors. Fresenius Environmental Bulletin, 12(9), 1025-1032.##Gürbüz, H., Kıvrak, E. and Sülün, A., 2002. Porsuk Göleti (Erzurum, Türkiye) bentik alg florası üzerinde kalitatif ve kantitatif bir araştırma. E.U. Journal of Fisheries andAquatic Sciences, 19(1-2), 53 - 61.##Hasırcı, S., 2012. Dodurga Baraj Gölü (Boyabat, Sinop) fitoplanktonu ve mevsimsel değişimi üzerine bir araştırma. Yüksek Lisans Tezi, Sinop Üniversitesi FenBilimleri Enstitüsü, Sinop, Turkey.##Hutchinson, G. E., 1967. A treatise on limnology, Introduction to lake biology and the limnoplankton. 115p. John Wiley and Sons. Inc, Newyork.##Kazancı, N., Girgin, S., Dügel, M. and Oğuzkurt, D., 1997. Türkiye iç suları araştırmalarıdizisi ıı (ed. n. kazancı): akarsuların çevre kalitesi yönünden değerlendirilmesindeve izlenmesinde biyotik indeks yöntemi. İmaj Yayınevi, Ankara, Turkey.##Kıvrak, E. and Gürbüz, H., 2005. Seasonal variations in phytoplankton composition andphysical-chemical features of Demirdöven Dam Reservoir, Erzurum, Turkey. Biologia, Bratislava, 60(1), 1- 8.##Lund, J. W. G. and Reynolds, C. S., 1982. The Development and Operation of LargeLimnetic Enclosures in Blelham Tarn, English Lake District, and Their Contribution to Phytoplankton Ecology. Progress in phycological research, 1, 1-65.##Maraşlıoğlu, F., 2007. Yedikır Baraj Gölü (Amasya-Türkiye) fitoplanktonu vemevsimsel değişimi üzerine bir araştırma. Doktora Tezi, Ondokuz MayısÜniversitesi Fen Bilimleri Enstitüsü, Samsun, Turkey.##Maraşlıoğlu, F., Soylu, E. N. and Gönülol, A., 2005. Seasonal variation of thephytoplankton of Lake Ladik, Samsun, Turkey. Journal of Freshwater Ecology, 20 (3), 549-554.##Özyalın, S. and Ustaoğlu, R., 2008. Kemer Baraj Gölü (Aydın) net fitoplankton komposizyonunun incelenmesi. E.U. Journal of Fisheries and Aquatic Sciens. 4, 275-282.##Pala, T.G. and Caglar, M., 2006. Keban Baraj Gölü Epilitik Diyatomelerive Mevsimsel Değişimleri. Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 18(3), 323-329.##Pala, G., 2007. Keban Baraj Gölü gülüşkür kesimindeki planktonik algler ve mevsimseldeğişimleri II-Bacillariophyta. Fırat Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 19(1), 23-32.##Palmer, C. M., 1969. A composite rating of algae tolerating organic pollution. Journal of Phycology and International Journal of Algal Research, 5 (1), 76-82.##Prescott, G. W., 1973. Algae of the western great lake area m.c. brown comp., Dubuque, Lowa, 997p.##Prygiel, J., Carpentier, P., Almeida, S., Coste, M., Druart, J.C., Ector, L., Guillard, D., Honoré, M.A., Iserentant, R., Ledeganck, P., Lalanne-Cassou, C., Lesniak, C.,Mercier, I., Moncaut. P., Nazart, M., Nouchet, N., Peres, F., Peeters, V., Rimet,F., Rumeau, A., Sabater, S., Straub, F., Torrisi, M., Tudesque, L., Van der Vijver,B., Vidal, H., Vizinet, J. and Zydek, N., 2002. Determination of the diatom index(IBD NF T 90-354): results of an intercalibration exercise. Journal of Applied Phycology, 14, 27-39.##Reynolds C. S., Huszar V. L., Nasellı-Flores L. and Melo S., 2002. Towards a functionalclassification of the freshwater phytoplankton. Journal of Phytoplankton Research, 24, 417-428.##Reynolds, C.S., 1993. The Ecology of freshwater phytoplankton. Chambridge Univ. 384p.##Rosenberg, D. M. and Resh, V. H., 1993. Introduction to freshwater biomonitoring andbenthic macroinvertebrates. p. 1-9. in: d.m. rosenberg and v.h. resh (eds.) freshwater biomonitoring and benthic macroinvertebrates, Chapman and Hall, New York.##Şahin, B., 1993. Trabzon - Uzungöl'ün algleri üzerinde bir araştırma. Doktora Tezi, Karadeniz Teknik Üniversitesi Fen bilimleri Enstitüsü, Trabzon, Turkey.##Şehirli, H., 1998. Akgöl (Terme-Samsun) fitoplanktonunun kompozisyonu vemevsimsel değişimi üzerinde bir araştırma. Yüksek Lisans Tezi, Ondokuz MayısÜniversitesi Fen Bilimleri Enstitüsü, Samsun, Turkey.##Sevindik, O, T., 2010. Phytoplankton composition of Çaygören Reservoir, Balıkesir, Turkey. Turkish Journal of Fischeries and Aquatic Sciences, 10, 295-304.##Sezen, G., 2008. Sarımsaklı Baraj Gölü (Kayseri) fitoplanktonu ve su kalitesi özellikleri. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Doktota Tezi, Ankara, Turkey.##Sömek, H., Balık, S. and Ustaoğlu, M.R., 2005. Topçam Baraj Gölü (Çine-Aydın) fitoplanktonu ve mevsimsel değişimleri. Süleyman Demirel Üniversitesi, Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1), 26-32.##Sorensen, T., 1948. A method of establishing groups of equal amplitude in plantsociology based on similarity of species and its application to analyses of the vegetation on Danishcommon. Kongelige Danske Videnskabernes Selskab 5 (4), 1-34.##Soylu, E. N. and Gönülol, A., 2006. 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Research Article: Evaluation of feed efficiency, growth and biochemical parameters of rainbow trout (Oncorhynchus mikyss) juveniles fed with different levels of Alphamune prebiotic 2 2 This research was conducted to evaluate the effect of different levels of Alphamune prebiotic on growth performance and blood chemistry of rainbow trout (Oncorhynchus mykiss) juvenile. Rainbow trout with an initial weight of 16.46±0.09 g were randomly assigned to four dietary treatments for eight weeks. The dietary treatment was: 0 (ALP0), 1 (ALP1), 1.5 (ALP1.5), and 2 (ALP2) g of Alphamune prebiotic/kg of basal diet. The results showed that there was no significant difference in survival rate and SGR at the end of the trial. A significant difference was observed in the final weight and weight gain, and the highest one was obtained in ALP1.5 treatment (p<0.05). Food conversion rate (FCR) was affected by Alphamune levels and the lowest FCR was observed in ALP1.5 treatment. Blood biochemistry assay revealed that glucose, triglyceride, and cholesterol were not influenced by Alphamune's different levels. There were significant differences in IgM and total protein and the highest value was obtained in ALP2. These results showed that 1.5% Alphamune prebiotic (ALP1.5) had a positive effect on growth performance and biochemical parameters of rainbow trout juveniles. 47 55 2021/05/22021/03/262021/06/102021/05/102021/04/29 1400/2/9 2021/07/232021/08/112021/08/112021/08/142021/08/15 1400/5/24 H Kanani H Kanani Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Iran kanani@yahoo.com S. R Javadian S. R Javadian Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Iran ro.javadian@gmail.com S Bahram S Bahram Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Iran s.bahram@qaemiau.ac.ir Alphamune Prebiotic Growth Oncorhynchus mykiss Aimrkolaie, A.K., Yansari A.T. and Khalesi, M.K., 2016. Calculation of protein and energy requirements in beluga sturgeon (Huso huso) using a factorial approach. Journal of Animal Physiology and Animal Nutrition, 97, 485-494.##Akrami, R., Ghelichi, A. and Gharaei, A., 2010. The use of prebiotics in aquaculture. Azadshahr. Journal of Fisheries, 4, 77-84.##Bolu, S.A., Ojo, V., Oyeleke, B.A., Ajiboye, A.O., Baa Sambo, A. and Oluyemi, O., 2009. Response of broiler chicks to graded levels of alphamune G supplementation. International Journal of Poultry Science, 8, 32-34.##Esmaeili, M., Abedian Kenari, A. and Rombenso, A., 2017. Effects of fish meal replacement with meat and bone meal using garlic (Allium sativum) powder on growth, feeding, digestive enzymes and apparent digestibility of nutrients and fatty acids in juvenile rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Aquaculture Nutrition, 23, 1225-1234.##Fooks, L.J. and Gibson, G.R., 2002. Probiotics as modulators of the gut flora. British Journal of Nutrition, 88(S1), 39- 49.##Ganguly, S., Dora, K.C., Sarkar, S. and Chowdhury, S., 2013. Supplementation of prebiotics in fish feed: a review. Reviews in Fish Biology and Fisheries, 23, 195-199.##Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, A., Salminen, J., Scott, k., Stanton, C., Swanson, K., Cani, P., Verbeke, K. and Reid, G., 2017. The international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature reviews gastroenterology and hepatology, 14, 491-502.##Hoseinifar, S.H., Esteban, M.A., Cuesta, A. and Sun, Y.Z., 2015a., Prebiotics and fish immune response: a review of current knowledge and future perspectives. Journal of Reviews in Fisheries Science & Aquaculture, 23(4), 315-328.##Hoseinifar, S.H., Khalili, M., Rostami, H.K. and Esteban, M.A., 2013. Dietary galactooligosaccharide affects intestinal microbiota, stress resistance, and performance of Caspian roach (Rutilus rutilus) fry. Fish & Shellfish Immunology, 35(5), 1416-1420.##Hoseinifar, S.H., Ringo, E., Shenavar Masouleh, A. and Esteban, M.A., 2014a. Probiotic, prebiotic and synbiotic supplements in sturgeon aquaculture: a review. Journal of Reviews in Aquaculture, 8(1), 89-102.##Huff, G.R., Huff, W.E., Rath, N.C., Tellez, G., 2006. Limited treatmant with B-1, 3/16 improves production values of broiler chickens challenged with Escherichia coli. International Journal of Poultry Science, 85, 613-618.##Li, P. and Gatlin, D.M. III., 2004. Dietary brewer's yeast and the prebiotic GroBiotic AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops × M. saxatilis) to Streptococcus iniae infection. Aquaculture, 231, 445-456.##Li, P. and Gatlin, D.M. III., 2005. Evaluation of the prebiotic GroBiotic-A and brewer's yeast as dietary supplements for sub-adult hybrid striped bass (Morone chrysops × M. saxatilis) challenged in situ with Mycobacterium marinum. Aquaculture, 248, 197-205.##Mahious, A.S., Gatesoupe, F.-J., Hervi, M., Metailler, R. and Ollevier, F., 2006b. Effect of dietary inulin and oligosaccharides as prebiotics for weaning turbot, Psetta maxima (Linnaeus, C. 1758). Aquaculture International, 14, 219-229.##Miandare, H.K., Farvardin, S., Shabani, A., Hoseinifar, S.H. and Ramezanpour, S.S., 2016. The effects of galactooligosaccharide on systemic and mucosal immune response, growth performance and appetite related gene transcript in goldfish (Carassius auratus gibelio). Fish & Shellfish Immunology, 55, 479-483.##Misra, C. K., Das, B. K., Mukherjee, S. C. and Pattnaik, P., 2006. Effect of multiple development of larval cobia. Aquaculture, 274(1), 148-152.##Mohammadzadeh, S., Noverian, A.H., Ouraji, H. and Falahatkar, B., 2017. Growth, body composition and digestive enzyme responses of Caspian Kutum, Rutilus frisii (Kamenskii, 1901), juveniles fed different levels of carbohydrates. Applied Ichthyology, 2017, 1-8.##Newman, K., 2007. Form follows function in picking MOS product. Feedstuffs, 79, 1-2.##Olsen, R.E., Myklebust, R., Kryvi, H., Mayhew, T.M. and Ringo, E., 2001. Damaging effect of dietary inulin to intestinal enterocytes in Arctic charr (Salvelinus alpinus L.). Aquaculture Research, 32, 931-934.##Piazzon, M.C., Calduch-Giner, J.A., Fouz, B., Estensoro, I., Simó-Mirabet, P., Puyalto, M., Karalazos, V., Palenzuela, O., Sitjà-Bobadilla, A. and Pérez-Sánchez, J., 2017. Under control: how a dietary additive can restore the gut microbiome and proteomic profile, and improve disease resilience in a marine teleostean fish fed vegetable diets. Microbiome, 5(1), p.164.##Pryor, G.S., Royes, J.B., Chapman, F.A. and Miles, R.D., 2003. Mannanoligosaccharides in fish nutrition: effects of dietary supplementation on growth and gastrointestinal villi structure in gulf of Mexico sturgeon. North American Journal of Aquaculture, 65, 106-111.##Ringo, E., 1998. Arctic charr, Salvelinus alpinus (L.), reared in fresh and sea water. An experimental study of lipid digestion and intestinal microflora. PhD thesis, Norwegian College of Fishery Science, University of Tromso.##Ringo, E., Olsen, R.E., Gifstad, T., Dalmo, R.A., Amlund, H., Hemre, G.I. and Bakke, A.M., 2010. Prebiotics in aquaculture: a review. Aquaculture Nutrition, 16, 117-136.##Sang, H.M. and Fotedar, R., 2010. Effects of dietary β-1,3-glucan on the growth, survival, physiological and immune response of marron, Cherax tenuimanus(smith, 1912). Fish & Shellfish Immunology, 28, 957-960.##Staykov, Y., Spring, P., Denev, S. and Sweetman, J., 2007. Effect of a mannan oligosaccharide on the growth performance and immune status of rainbow trout (Oncorhynchus mykiss). Aquaculture International, 15, 153-161.##Torrecillas, S., Makol, A., Caballero, M.J., Montero, D., Gines, R., Sweetman, J. and Izquierdo, M.S., 2011a. Improved feed utilization, intestinal mucus production and immune parameters in sea bass (Dicentrarchus labrax) fed mannan oligosaccharides (MOS). Aquaculture Nutrition, 17, 223-233.##Torrecillas, S., Makol, A., Caballero, M.J., Montero, D., Robaina, L., Real, F., Sweetman, J., Tort, L. and Izquierdo, M.S., 2007. Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides. Fish and Shellfish Immunology, 23, 969-981.##Yousefian, M., Hedayatifard, M., Fahimi, S., Shikholeslami, M., Irani, M., Amirina, C. and Mousavi, S.E., 2012. Effect of prebiotic supplementation on growth performance and serum biochemical parameters of kutum (Rutilus frisii kutum) fries. Asian Journal of Animal and Veterinary Advances, 7, 684-692.##Zhongzhen, L., Ngoc T.T., Peina. J., Zaiqiao, s., Xiaobo, W. and Shengkang, B. 2019. Effects of prebiotic mixtures on growth performance, intestinal microbiota and immune response in juvenile chu's croaker, Nibea coibor, Fish and shellfish immunology, 89, 564-573.##Zhou, C., Liu, B., Wang, G., Xie, J., Ge, X. and Su, Y., 2009a. Effects of the compound of oligosaccharide and Chine medicines and flavomycin on growth disease resistance of allogynogenetic crucian carp (Carassius auratus gebelio var. E'erqisi, Bloch). Freshwater Fisheries, 39, 44-51.## ##

Research Article: Effects of nanoparticles of dried Aloe vera extract on some of the hematological parameters, liver enzymes and immune responses in Siberian sturgeon (Acipenser baerii) 2 2 Today, nanotechnology is considered as a priority and strategic technology for all countries due to its wide range of applications. The present study was performed to evaluate the effects of various levels of dried Aloe vera extract nanoparticles on some hematological parameters, liver enzymes and immune responses of Siberian sturgeon (Acipencer baerii). Three hundred sixty Acipenser baerii (with mean weight of 10.95 ± 0.04 g), were randomly divided into three treatment groups including 0.5%, 1% and 1.5% of nanoparticles of dried Aloe vera extract and one control group (without any additive), each in three replicates, stored in twelve tanks of 500-liter fiberglass with 350 liters water. Water physicochemical parameters were recorded daily. Nanoparticles of dried A. vera extract in a ratio of 0.5%, 1%, and 1.5% were added to the treatment groups diet for 2 months. At the end of the first and second months of rearing, the necessary samplings for hematology and serology studies were done. The results of this study showed that the amounts of RBC, Hb, Hct, MCV, WBC, lysozyme, ACH50 and IgM were significantly different in the treatment groups compared with the control group at the end of the study (p<0.05). There were no significant differences in liver enzymes including ALT, AST and ALP between treatments and control groups (p>0.05). In conclusion the results have shown that use of nanoparticles of dried A. vera extract had the impact of immune function booster and the usage of this nanoparticle mainly at the level of 1% in the diet can be used as a safety stimulant for Siberian sturgeon. 56 69 2021/05/22021/03/262021/06/102021/05/102021/04/292021/06/11 1400/3/21 2021/07/232021/08/112021/08/112021/08/142021/08/152021/08/16 1400/5/25 S Bazari Moghaddam S Bazari Moghaddam International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran Iran soheilbm274@gmail.com M Sharif Rohani M Sharif Rohani Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran Iran mostafasharif@yahoo.com M Haghighi M Haghighi Cold-water Fishes Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Tonekabon, Iran Iran masoud126@yahoo.com A. vera Nanoparticles Hematological parameters Immune indices Siberian sturgeon Adamek, Z., Prokes, M.M, Barus, V. and Sukop, I., 2007. 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