eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 1 10 article A Dashtiannasab 1 M Mesbah 2 R Pyghan 3 S Kakoolaki 4 Aquatic Health and Disease Department, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Org., Bushehr, Iran Department of Clinical Sciences, Shahid Chamran University, Ahwaz, Iran Department of Clinical Sciences, Shahid Chamran University, Ahwaz, Iran Aquatic Health and Disease Department, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Org., Tehran, Iran Shrimp aquaculture has expanded rapidly in many countries and this rapid development resulted diseases outbreaks and further considerable utilization  of antibiotics. Use of natural products as antimicrobial has been reported as a resolution problem. The crude extract of a red seaweed (Laurencia snyderiae) collected from the Persian Gulf was evaluated for shrimp growth performance and to determine in vivo efficacy of this seaweed to prevention of  shrimp Vibriosis. The ethanol extract of Laurencia snyderiae was not toxic to the Artemia in star- I when it was fed to them for enrichment.  The white shrimp (Litopenaeus vannamei) Juveniles fed with these enriched Artemia at 0 mg mL-1 (Control group), and three treatments of 200 , 400   and 600 mg mL-1  for a 30 days period. Results showed an increase in survival rate in treatment groups compared with control group but wasn’t significantly (P<0.05). Shrimps that fed with enriched Artemia showed a significant in growth parameters compare ing to  Control group (P<0.05). The notably lower mortality was observed when these juvenile shrimps were challenged to Vibrio harveyi (after 30 days)  in comparison with  Control. The  results indicated that EELS has a good potential in growth- promoting and antibacterial activity against V. harveyi in which  is useful  in shrimp aquaculture. http://ijaah.ir/article-1-93-en.pdf red seaweed Laurencia snyderiae Litopenaeus vannamei Vibrio harveyi eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 11 16 article A Hedayati 1 AP Salati 2 Department of Fisheries , Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Department of Fisheries, Faculty of Marine Natural Resources, Khoramshar University of Marine Science and Technology, Khorramshahr, Iran Different pollutants have undesirable effects on aquatic ecosystems, detergent powder is one of the elements in the sewages so study on the effects of this factor on  aquatic animals is crucial. The aim of the present study was to investigate acute effects of detergents as potentially dangerous additive to assess mortality effects of this product on some cultured fish. Silver Carp (Hypophthalmicthys moitrix), Common Carp (Cyprinus carpio) ,Goldfish (Carassius auratus) and Roach (Rutilus rutilus caspicus) were exposed to the 0, 2, 10, 20 and 40 mg L-1  of detergent powder. All experiments were done according the standard methods for 96 h  exposure. LC50 was determined by probit analysis. The 96h toxicity tests showed 100% mortality at 40 mg L-1, and no mortality was observed in 5 mg L-1  for all species. LC50 of detergent powder in Goldfish (24.6 ± 0.32 mg L-1) was higher than other species, while silver carp (16.7 mg L-1) had the lowest one. http://ijaah.ir/article-1-94-en.pdf Fish LC50 Toxicity test Detergent powder per Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 17 23 article S kakoolaki 1 M Afsharnasab 2 Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extention Org., Tehran,Iran Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extention Org., Tehran,Iran Development of shrimp farming has been associated with the incidence of fatal diseases including viral, bacterial and protozoan parasites of shrimp. Our study was aimed to present an status of several important protozoan parasites of F. indicus in cultured earthen ponds in Iran during the period of 2011 to 2012. One hundred live shrimp  were randomly prepared from the Shrimp Helleh Station in south of Iran. Prevalence and intensity were two important indices that calculated in our research. They were scraping, putting on clean slides and examined under microscope to observe the protozoan parasites. Zoothamnium sp. is more prevalent pathogen among peritrichous ciliates (88.66% to 79%) in pleopods. Acineta sp. was less prevalent in our isolations neither in pleopods nor in gills. It is concluded that, peritrichous ciliates are more frequent ciliates causing mortality in cultured shrimps at high density in higher temperature and ammonia.  http://ijaah.ir/article-1-111-en.pdf F. indicus Protozoan Epibiont ciliates Epicommensal eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 24 36 article M Mesbah 1 T Mohammadian 2 m Alishahi 3 A Jangaran nejad 4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran A feeding trial was conducted for sixty continuous days to determine the effect of dietary Aloe vera and Echinacea  on immunological parameters of Shirbot (Barbus grypus) Four hundred and twenty fish weighing 50-60 g were randomly subjected to to seven different treatment, including one control  (Aloe vera and Echinacea free diet), A1 (0.1% of Aloe vera, A2 (0.2% of Aloe vera  , A3 (0.5% of Aloe vera) ,  E1 (0.1% of Echinacea) , E2 (0.2% of Echinacea  ), E3 (0.5% of Echinacea) each in triplicate. Some immunological parameters such as respiratory burst activity, Complement activity, Lysozyme activity, serum bactericidal activity, total protein and albumin were assessed. At the end of treatment, 30 fish from each group were challenged with Aeromonas hydrophila . No significance difference was seen in respiratory burst activity of  Aloe vera and Echinacea treatments compared to the control group (P>0.05).Complement and serum bactericidal activity enhanced in all the Aloe vera groups (P<0.05). Total protein and globulin in Aloe vera and Echinacea  treatments decreased compared with control group (P>0.05). Lysozyme activity in 0.2% Aloe vera treatments was significantly higher than Echinace and control groups (P<0.05). According to obtained results, it might be concluded that the feeding of this species by Aloe vera (specifically 0.2%) extract could likely enhance immunological parameters better than Echinacea . http://ijaah.ir/article-1-97-en.pdf Aloe vera Barbus grypus Echinacea immunological parameters eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 37 44 article L Roomiani 1 M Ghaeni 2 AE khajerahimi 3 Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Department of Fisheries, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Department of Fisheries, Tehran North Branch, Islamic Azad University, Tehran, Iran The major objective of this study was to investigate heavy metal content of black pomfret (Parastromateus niger ). The concentrations of Zn, Cu, As and Cd were determined in external (gill and shell) and internal organs (muscle and liver) of black pomfret in northwest of Persian Gulf in summer 2013. A total of 60 specimens were collected from fishing bottom trawlers. Concentrations of these heavy metals were measured by using atomic absorption spectrometer. Results showed that heavy metals concentrations varied significantly, depending on the types of the tissue in fish. Bioaccumulation of heavy metals Zn and Cu were in the sequence liver> shell> gill> muscle but for Cd and As were in the sequence of gill> shell> liver> muscle. In total organs and among heavy metals the accumulation of Zn and As were the highest and the lowest (P< 0.05). The results indicated that the gill of fishes were highly contaminated by heavy metals and exceeded of WHO and NHMRC guidelines for As and Cd, respectively. http://ijaah.ir/article-1-98-en.pdf Parastromateus niger Bioaccumulation Heavy metals Persian Gulf eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 45 53 article A Samnejhad 1 M Afsharnasab 2 S Kakoolaki 3 Specialized veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Aquatic Animal Health & Diseases, Agricultural Research, Education and Extention Org., Iranian Fisheries Science Research Institute, Tehran, Iran Department of Aquatic Animal Health & Diseases, Agricultural Research, Education and Extention Org., Iranian Fisheries Science Research Institute, Tehran, Iran In  this  research,  the  effect  of  different  water  temperature  on  THC  (Total  hemocyte  count),  variety  of  hemocyte  cells  and  histopathological  differences    in  Crayfish  Astacus leptodactylus  were  studied.  This  study  was  designed  in  three  groups  in  triplicate  with  20  Crayfish  in  each  glass  aquarium.  The  rate  of  temperature  in  three  groups  were  10,15,  20,  and  25 °C, respectively.  The  results  indicated  that  the  differences  of  THC  value  between  Group1  (69±  19.00)    and  Group  3  (18±  12.49)  were  significant  after  48  h  .  After  168  h,  also  the  differences  of  THC  value  between  Group  3  (9±  6.24)  with  Groups 1 & 2  (35.33±  7.02 &32.33±  8.73)  were  significant.  The  highest  and  lowest  density  of    hemocyte  cells  were belong to semigranular  cells  (SGC),  granular  cells  (GC)  and  hyaline  cells  (HC), with estimated values of  59-64%,  28-36%  and  3-8%  respectively.  The  result  of  histopathology  in  heaptopancreas,  gill  and  heart  in  Group 3  affecting  of  high  temperature  showed  the  distribution  of  hemocyte  aggregation  and  pyknosis  of  nucleus  within  vaculation  of  hepatopancreas.  In  Groups  1  and  2  the  differences  of  hepatopancreas  and  gill  lamella  were  lower  and  no  pathological  changes  of  heart  were  observed. No significant changes of digestive tract were observed in all treatments. http://ijaah.ir/article-1-99-en.pdf Astacus leptodactylus Total hemocyte count Histopathology eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 54 65 article M Soltani 1 A Shenavar Masouleh 2 M Ahmadi 3 M Pourkazemi 4 A Taherimirghaed 5 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran; Center of excellence of aquatic animal health, University of Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran; Center of excellence of aquatic animal health, University of Tehran, Iran International Sturgeon Research Institute, Rasht, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran; Center of excellence of aquatic animal health, University of Tehran, Iran International Sturgeon Research Institute, Rasht, Iran Growth behavior of five lactic acid bacteria previously isolated from Persian sturgeon (Acipenser persicus), were evaluated at different pH, temperature and salt concentrations. Also, antibacterial activity of extracellular products (ECPs) of this LAB were assessed to Aeromonas hydrophila . Further, their antibiotic susceptibility was determined with some commonly used antibiotics in aquaculture. In an in vivo work the effect of Lactococcus lactis  as the supplementary diet was evaluated on growth performance of Persian sturgeon for a period of 56 days. Strong growth of all LAB were seen at 20 and  300 C, as well as 40 and 80 g L-1 NaCl. The LAB ECPs exhibited varied results of antagonism to the A. hydrophila  with maximum activity observed at temperature between 25 and  300 C Also, the higher antagonistic activity was observed for ECPs of W. cibaria , P. pentosaceus, and L. lactis   at pH 9. Both  W. cibaria and E. faecalis  were resistance to oxytetracycline, erythromycin, trimethoprim sulfamethoxazol, enrofloxacin, florfenicol, and flumequine, while L. lactis  was sensitive to oxytetracycline, erythromycin, enrofloxacin, florfenicol. These results showed that use of L. lactis  can act as a positive probiotic in Persian sturgeon feed via improvement of fish growth performance, feed efficiency and fish health. http://ijaah.ir/article-1-100-en.pdf sturgeon health Lactococcus lactis probiotic. eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 66 72 article A Taheri Mirghaed 1 M Ghadam 2 H Ebrahimzadeh Mousavi 3 M Soltani 4 P Shohreh 5 H Rahmati Holasoo 6 I Mobedi 7 A Banitalebi 8 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Parasitology, Faculty of Hygiene, Tehran University of Medical Sciences, Tehran, Iran Department of Aquatic Animals Health & Diseases, Hormozgan Veterinary Organization, Bandar Abbas, Iran Pick handle barracuda (Sphyraena jello) ) is one of the most important commercial species in the Persian Gulf. In order to investigate prevalence and intensity of nematoda, 150 S. jello  from three sites (Khuzestan, Bushehr and Hormozgan provinces) of the Iranian coast of the Persian Gulf were monthly investigated in the period 2012-2013. The nematodes belonging to the genus Hysterothylacium (family Raphidascarididae) were isolated from the abdominal cavity and digestive tract with a prevalence of 12% and mean intensity of 4.8. Intensity of infestation was increased with size of the host (P<0.05). This study is the FIrst report of presence of the third and fourth- stage of an unreported type of the third and fourth- stage of an unreported type of Hysterothylacium  larvae in S. jello  from the Persian Gulf. http://ijaah.ir/article-1-101-en.pdf Sphyraena jello Hysterothylacium sp. nematodes Persian Gulf. eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 73 79 article H Rahmati-Holasoo 1 F Isfahani 2 A rostami 3 A Vajhi 4 HA Ebrahimzadeh Mousavi 5 M Sattari 6 Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Fishery, Faculty of Animal Sciences and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Department of Fishery, Faculty of Natural Resources, Guilan University , Somehsara, Iran Skeletal deformities cause severe economic losses in the aquaculture industry due to the reduced market value of the affected fish. The banded cichlid (Heros severus) is an ornamental cichlid native to the Amazon region. In the summer 2011 during clinical investigation on 750 severum of 5 ornamental fish farms in Karaj and Tehran, Iran, a two-year-old severum having deformity in dorsal and anal  fins  was referred to the radiology section of Veterinary Teaching Hospital. Standard, high quality radiographs were taken and studied. Deformities in vertebrates, plural ribs, neural spines and hemal spines were detected. Kyphosis and Lordosis was observed in V14 to V18. The scoliosis in both parts was left and right sided and in first part was detectable in V10 to V13 and in second in V24 to V29. Neural spines of V14 to V17 and hemal spines of V16 to V22 were deformed. This case history showed that parents of this fish were offspring’s of the same fish and implies that inbreeding of brood stock may cause such deformities. http://ijaah.ir/article-1-102-en.pdf Heros severus skeletal deformities Kyphosis Lordosis scoliosis. eng Iranian Association of Aquatic Animal Health Sustainable Aquaculture and Health Management Journal 2821-1383 2821-1383 2016-02 2 1 80 86 article MY Yahyazadeh 1 M Seidgar 2 S Shiri 3 National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Urmieh, Iran National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Urmieh, Iran National Artemia Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Urmieh, Iran         Freshwater crayfish (Astacus leptodactylus) is an important economic fisheries resource of Aras reservoir, Iran. The infection of freshwater crayfish by gram negative and gram positive bacteria are common in natural and cultural environments. These bacteria are considered as secondary disease agents or opportunities. This study was conducted to investigate infestation condition on 70 freshwater crayfish regarding the bacterial agents by culturing from haemolymph in aseptic condition on Blood Agar, Trypton Soya Agar (TSA) and Cytophaga Agar and was performed by biochemical tests. In this study Aeromonas hydrophila, Staphylococcus aureus, Micrococcus luteus and Flavobacterium johnsoniae were isolated and determined. According to the results which was performed for first time on Aras reservoir freshwater crayfish, the percentage of crayfish which carried the bacteria in haemolymph were variable (20-58/5%). Also, the gram negative bacteria particularly Aeromonas hydrophila were dominant among the bacterial isolates from crayfish. http://ijaah.ir/article-1-103-en.pdf bacterial infestation Astacus leptodactylus Aras reservoir West Azarbaijan Iran