Sustainable Aquaculture and Health Management Journal نشریه آبزی پروری پایدار و مدیریت بهداشتی Sustainable Aquaculture. Health. Management. J. Agriculture http://ijaah.ir 1 admin 2821-1383 2821-1383 10.61186/ijaah 14 13 en jalali 1394 11 1 gregorian 2016 2 1 2 1 online 1 fulltext
en Effect of water temperature on hemocytes and histopathological findings in freshwater Crayfish (Astacus leptodactylus) hematology hematology پژوهشي Original research papers <p dir="ltr" style="text-align: justify;">In&nbsp; this&nbsp; research,&nbsp; the&nbsp; effect&nbsp; of&nbsp; different&nbsp; water&nbsp; temperature&nbsp; on&nbsp; THC&nbsp; (Total&nbsp; hemocyte&nbsp; count),&nbsp; variety&nbsp; of&nbsp; hemocyte&nbsp; cells&nbsp; and&nbsp; histopathological&nbsp; differences&nbsp;&nbsp;&nbsp; in&nbsp; Crayfish&nbsp; <em>Astacus leptodactylus&nbsp; </em>were&nbsp; studied.&nbsp; This&nbsp; study&nbsp; was&nbsp; designed&nbsp; in&nbsp; three&nbsp; groups&nbsp; in&nbsp; triplicate&nbsp; with&nbsp; 20&nbsp; Crayfish&nbsp; in&nbsp; each&nbsp; glass&nbsp; aquarium.&nbsp; The&nbsp; rate&nbsp; of&nbsp; temperature&nbsp; in&nbsp; three&nbsp; groups&nbsp; were&nbsp; 10,15,&nbsp; 20,&nbsp; and&nbsp; 25 &deg;C, respectively.&nbsp; The&nbsp; results&nbsp; indicated&nbsp; that&nbsp; the&nbsp; differences&nbsp; of&nbsp; THC&nbsp; value&nbsp; between&nbsp; Group1&nbsp; (69&plusmn;&nbsp; 19.00)&nbsp; &nbsp;&nbsp;and&nbsp; Group&nbsp; 3&nbsp; (18&plusmn;&nbsp; 12.49)&nbsp; were&nbsp; significant&nbsp; after&nbsp; 48&nbsp; h &nbsp;.&nbsp; After&nbsp; 168&nbsp; h,&nbsp; also&nbsp; the&nbsp; differences&nbsp; of&nbsp; THC&nbsp; value&nbsp; between&nbsp; Group&nbsp; 3&nbsp; (9&plusmn;&nbsp; 6.24)&nbsp; with&nbsp; Groups 1 & 2&nbsp; (35.33&plusmn;&nbsp; 7.02 &32.33&plusmn;&nbsp; 8.73)&nbsp; were&nbsp; significant.&nbsp; The&nbsp; highest&nbsp; and&nbsp; lowest&nbsp; density&nbsp; of&nbsp;&nbsp;&nbsp; hemocyte&nbsp; cells&nbsp; were belong to semigranular&nbsp; cells&nbsp; (SGC),&nbsp; granular&nbsp; cells&nbsp; (GC)&nbsp; and&nbsp; hyaline&nbsp; cells&nbsp; (HC), with estimated values of&nbsp; 59-64%,&nbsp; 28-36%&nbsp; and&nbsp; 3-8%&nbsp; respectively.&nbsp; The&nbsp; result&nbsp; of&nbsp; histopathology&nbsp; in&nbsp; heaptopancreas,&nbsp; gill&nbsp; and&nbsp; heart&nbsp; in&nbsp; Group 3&nbsp; affecting&nbsp; of&nbsp; high&nbsp; temperature&nbsp; showed&nbsp; the&nbsp; distribution&nbsp; of&nbsp; hemocyte&nbsp; aggregation&nbsp; and&nbsp; pyknosis&nbsp; of&nbsp; nucleus&nbsp; within&nbsp; vaculation&nbsp; of&nbsp; hepatopancreas.&nbsp; In&nbsp; Groups&nbsp; 1&nbsp; and&nbsp; 2&nbsp; the&nbsp; differences&nbsp; of&nbsp; hepatopancreas&nbsp; and&nbsp; gill&nbsp; lamella&nbsp; were&nbsp; lower&nbsp; and&nbsp; no&nbsp; pathological&nbsp; changes&nbsp; of&nbsp; heart&nbsp; were&nbsp; observed. No significant changes of digestive tract were observed in all treatments.</p> Astacus leptodactylus, Total hemocyte count, Histopathology 45 53 http://ijaah.ir/browse.php?a_code=A-10-35-27&slc_lang=en&sid=1 A Samnejhad 10031947532846001397 10031947532846001397 Yes Specialized veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran M Afsharnasab 10031947532846001398 10031947532846001398 No Department of Aquatic Animal Health & Diseases, Agricultural Research, Education and Extention Org., Iranian Fisheries Science Research Institute, Tehran, Iran S Kakoolaki 10031947532846001399 10031947532846001399 No Department of Aquatic Animal Health & Diseases, Agricultural Research, Education and Extention Org., Iranian Fisheries Science Research Institute, Tehran, Iran