en Treatment stategies with herbal or others Treatment stategies with herbal or others پژوهشي Original research papers <p align="justify"><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">This study was conducted to examine </font></font></p> <p><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">in vitro </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">antibacterialpotential from seed methanol extract of Peganum harmala (L) against some </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">fi</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">sh pathogenic bacteria including Lactococcus garvieae, Aeromonas hydrophila, </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">Yersinia ruckeri </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">and </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">Pseuodomonas putida </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">isolated from diseased rainbow trout (</font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">Oncorhynchus mykiss</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">). The antibacterial activity of extracts was evaluated using disc diffusion assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). MICS were measured by serial dilution and the microplate assays. Results showed that the methanol extract of </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">P. harmala </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">was bactericidal for all test acteria. The MICs of extract using serial dilution and microplate method were 0.6 mg mL</font></font><font face="TimesNewRomanPSMT" size="1"><font face="TimesNewRomanPSMT" size="1">-1 </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">and 0.312 to 0.625 mg mL</font></font><font face="TimesNewRomanPSMT" size="1"><font face="TimesNewRomanPSMT" size="1">-1 </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">against </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">P. putida </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">and 0.8 mg mL</font></font><font face="TimesNewRomanPSMT" size="1"><font face="TimesNewRomanPSMT" size="1">-1 </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">and 0.625 to 1.25 mg mL</font></font><font face="TimesNewRomanPSMT" size="1"><font face="TimesNewRomanPSMT" size="1">-1 </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">against </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">L. garvieae</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">, </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">A. hydrophila</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">, </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">Y. ruckeri</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">, respectively which was con</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">fi</font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">rmed by MBC determination. Thus, the antibacterial activity of seed extract of </font></font><i><font face="TimesNewRomanPS-ItalicMT" size="3"><font face="TimesNewRomanPS-ItalicMT" size="3">P. harmala </font></font><font face="TimesNewRomanPSMT" size="3"><font face="TimesNewRomanPSMT" size="3">can be comparable as an alternative in the control of infectious by these Microorganisms.</font></font></i></i></i></i></i></i></i></i></i></i></p> fish pathogenic bacteria Peganum harmala, seed extracts, 7 16 http://ijaah.ir/browse.php?a_code=A-10-35-11&slc_lang=en&sid=1 P. Akbary 10031947532846001050 10031947532846001050 Yes Department of Marine Sciences, Chabahar Maritime University, Iran M. S. Fereidouni 10031947532846001051 10031947532846001051 No Aquatic Animal Health Unit, School of Veterinary Medicine, Shiraz University, Shiraz, Iran M. Akhlaghi 10031947532846001052 10031947532846001052 No Aquatic Animal Health Unit, School of Veterinary Medicine, Shiraz University, Shiraz, Iran