en Viral Diseases and viology Viral Diseases and viology پژوهشي Original research papers <div style="text-align: justify;">The objectives to this research were to determine the haematological changes in <em>Oryctolagus cuniculus</em> as an animal model in response to killed hemorrhagic septicemia virus (VHSV) virulent to <em>Oncorhynchus mykiss</em>. This study composed of 5 groups; Treatment 1 (T1) with 1cc virus, treatment 2 (T2) with 0.5cc virus and 0.5cc adjuvant, treatment 3 (T3) with 0.75cc virus and 0.25cc adjuvant, treatment 4 (T4) with 0.25cc virus and 0.75cc adjuvant, and a control for a period of 4 months, once a month IM injection in 5 group of three New Zealand rabbits with health certification prepared by Razi Vaccine and Serum Research Institute. After 15 days, blood parameters, hematocrit (HCT), hemoglobin (HGB), mean hemoglobin concentration in red blood cells (MCH), red blood cells (RBC), average concentration of red blood cell (MCHC), white blood cells (WBC), neutrophils (SEG), lymphocytes (Lyn), eosinophil (Eos), aspirate transaminase (AST) and alanine transaminase (ALT).The results&nbsp; showed that ALT, AST, HCT, HGB and WBC concentrations of all rabbits significantly were difference (<em>p < 0.05</em>). T2 (0.5cc virus and 0.5cc adjuvant) and T3 (0.75cc virus and 0.25cc adjuvant) showed higher ALT, AST, HCT and WBCs among other treatments in all 4 blood samplings and among these treatments, T2 with lower levels of ALT and AST (indicating less pressure on the liver) and higher levels of WBCs which can lead to greater amount of neutralizing polyclonal antibodies indicated better results.</div> <div style="text-align: justify;"><br> &nbsp;</div> Viral Hemorrhagic Septicemia virus, Rabbit, Hematology, Enzymes 61 77 http://ijaah.ir/browse.php?a_code=A-10-387-1&slc_lang=en&sid=1 M Hafezieh Masoumehhafezieh@yahoo.com 10031947532846002941 10031947532846002941 No Islamic Azad University, Science and Research Branch, Tehran, Iran S Kakoolaki bsh443@gmail.com 10031947532846002942 10031947532846002942 Yes Iran Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran M Ghasemi mohades@yahoo.com 10031947532846002943 10031947532846002943 No Inland Water Aquaculture Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Bandar Anzali, Iran R Kazempoor rkbs_kh@yahoo.com 10031947532846002944 10031947532846002944 No Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran A Hemati bsh443@gmail.com 10031947532846002945 10031947532846002945 No Iran Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran