Sustainable Aquaculture and Health Management Journal
نشریه آبزی پروری پایدار و مدیریت بهداشتی
Sustainable Aquaculture. Health. Management. J.
Agriculture
http://ijaah.ir
1
admin
2821-1383
10.52547/ijaah
14
13
en
jalali
1397
1
1
gregorian
2018
4
1
4
1
online
1
fulltext
en
An inactivated infectious pancreatic necrosis virus immersion vaccine used in rainbow trout (Oncorhynchus mykiss) (Walbaum, 1792) and the effect of Montanide IMS 1312 VG as adjuvant on the vaccine efficacy
Vaccination
Vaccination
پژوهشي
Original research papers
<div style="text-align: justify;">Since infectious pancreatic necrosis virus (IPNV) is the most predominant strain causing fatal viral disease in Iranian fish farms, an inactivated vaccine for this fatal pathogen was developed. Additionally, the effect of the above-mentioned vaccine was investigated against IPNV using Montanide 1312 VG adjuvant. Two hundred and forty healthy fish with an average weight of 3.00±0.45 g were vaccinated using immersion method with ethylene amine-inactivated IPN vaccine in combination with Montanide IMS 1312 VG for 5 min at 11 ± 1°C. The control group was vaccinated with sterile phosphate-buffered saline. The immersed vaccine with Montanide IMS 1312 VG adjuvant increased the expression of immune system genes such as IgM, MX, and CD8 within 30 days of vaccination period. The expression of IgM, Mx (P<0.05) and CD8 (P>0.05) genes was higher for the vaccine containing 10g of Montanide than that of 50g of Montanide. In fish vaccinated with the adjuvant, the relative percent survival was notably higher than of fish vaccinated without the adjuvant and the control group. Results showed that the ethylene amine-inactivated IPNV vaccine by the immersion method with Montanide IMS 1312 VG significantly has a strong protective immunity against IPNV in rainbow trout.</div>
Infectious pancreatic necrosis virus, Rainbow trout, Immersion vaccine, Montanide IMS 1312 VG
69
81
http://ijaah.ir/browse.php?a_code=A-10-222-1&slc_lang=en&sid=1
S
Rashidi Monfared
سمیرا
رشیدی منفرد
rashidi_s@ut.ac.ir
10031947532846002036
10031947532846002036
No
Department of Veterinary, Shiraz university, Shiraz, Iran
دانشکده دامپزشکی دانشگاه شیراز
M
Akhlaghi
مصطفی
اخلاقی
akhlaghi@shirazu.ac.ir
10031947532846002037
10031947532846002037
Yes
Department of Veterinary, Shiraz university, Shiraz, Iran
دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران
S. A
Pourbakhsh
poursaba@yahoo.com
10031947532846002038
10031947532846002038
No
Razi Vaccine and Serum Research Institute, Karaj, Iran
موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران
R
Fallahi
روزبه
فلاحی
r.fallahi@rvsri.ac.ir
10031947532846002039
10031947532846002039
No
Razi Vaccine and Serum Research Institute, Karaj, Iran
موسسه تحقیقات واکسن و سرم سازی رازی، کرج، ایران
S
Soltanian
سیاوش
سلطانیان
siyavashsoltanian@yahoo.com
10031947532846002040
10031947532846002040
No
Department of Veterinary, Shiraz university, Shiraz, Iran
دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران
A
Yektaseresht
آزاده
یکتاسرشت
yektaseresht@yahoo.com
10031947532846002041
10031947532846002041
No
Department of Veterinary, Shiraz university, Shiraz, Iran
دانشکده دامپزشکی دانشگاه شیراز، شیراز، ایران