en Aquaculture and Health management Aquaculture and Health management پژوهشي Original research papers <p style="text-align:justify;line-height:150%"><span style="font-size:11.0pt; line-height:150%;letter-spacing:-.1pt">Applying anaesthetic agent is recommended in aquaculture during fish handling specially tagging. However, it should be noted that anesthesia itself is likely to induce stressful condition due to its improper and high concentration. By means of nanoemulsions, drug delivery improves which in turn contributes to fewer amounts of pharmaceutical agents&rsquo; usage. In this research, nanoemulsions of clove oil (NCL) and lemonbeebrush (NLB) were used to develop the anesthetic efficacy and reduce the concentration needed for anesthesia in <i>Huso huso</i> (mean weight: 1115 &plusmn; 242.25 g) as an endangered species. Based on the results, anesthesia induction time (s) of stage 3 & 4 was significantly decreased in NCL and NLB when compared to MS-222 (p<0.05).&nbsp;</span><span style="font-size: 11pt;">NCL treated beluga were induced stage 5 anesthesia much quicker than NLB and MS-222. In contrary, recovery time did not show significant difference among treatments (p>0.05). Among measured biochemical factors, lactate had the highest value in NCL group during anesthesia (p<0.05). In contrast, lactate level had highest level in NLB, 24h post anesthesia (p<0.05). NLB and NCL produced higher non-specific immune (Lysozyme and ACH50) enhancements at 0 and 24h post anesthesia. Furthermore, results showed that MS-222 induced increased serum cortisol levels (p<0.05). Cortisol and glucose were not depressed by NLB and NCL, suggesting that these anesthetics are safer anesthetic for </span><i style="font-size: 11pt;">Huso huso</i><span style="font-size: 11pt;"> as it does not cause immune depression in anesthetized fish.</span></p> <p style="margin:0cm;margin-bottom:.0001pt;text-align:justify;line-height:150%; background:white"><span style="font-size:11.0pt;line-height:150%"></span></p> Aloysia triphylla, Eugenia caryophyllata, Innate immunity, Anesthesia, Great sturgeon 109 123 http://ijaah.ir/browse.php?a_code=A-10-219-1&slc_lang=en&sid=1 H Gholipour Kanani حسنا قلی پور کنعانی gholipourk@gmail.com 10031947532846002066 10031947532846002066 Yes Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University, Golestan province, Iran دانشگاه گنبدکاووس P Hooshmand پریا هوشمند pariahooshmand71@gmail.com 10031947532846002067 10031947532846002067 No Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University, Golestan province, Iran دانشگاه گنبدکاووس M Harsij محمد هرسیج m_harsij80@yahoo.com 10031947532846002068 10031947532846002068 No Department of Fisheries, Faculty of Agriculture and Natural Resource, Gonbad Kavous University, Golestan province, Iran دانشگاه گنبدکاووس E Gholamalipour Alamdari ابراهیم غلامعلی‌پور علمداری eg.alamdari@gmail.com 10031947532846002069 10031947532846002069 No Department of Plant Production, College of Agriculture and Natural Resources, Gonbad Kavoue University, Gonbad Kavous, Golestan province, Iran دانشگاه گنبدکاووس