Sustainable Aquaculture and Health Management Journal

en Evaluation of antimicrobial activity of peptides isolated from Cerastoderma and Didacta bivalves habitat in the southern shores of the Caspian Sea Bacterial Disease Bacterial Disease پژوهشي Original research papers <div style="text-align: justify;">The antibacterial effects of methanol, ethanol, chloroform extracts and alcalase hydrolysis of Cerastoderma and Didacta were investigated against Salmonella typhi, Salmonella paratyphi and Staphylococcus aureus by disk diffusion method, Minimum Inhibitory Concentration and Minimum Bactericidal Concentration. Methanolic and ethanolic extracts of Cerastoderma showed the highest effects against S. typhi (20.33 ± 0.33 and 12.33 ± 0.33) and S. paratyphi (22.66 ± 1.45 and 15.33 ± 0.33), however, the same effects against S. aureus (18.00 ± 0.00 and 17.00 ± 0.00) were observed for two bivalves. Chloroform extract of Cerastoderma and Didacta showed similar effects in controlling S. paratyphi (8.00 ± 0.58 vs 10.00 ± 0.57) and S. aureus (16.00 ± 1.15 vs 16.00 ± 1.15) in concentrations of 10 and 5 mg ml-1. Chloroform extract of Cerastoderma exhibited higher effect than that from Didacta against S. typhi (11.00 ± 1.73 vs 8.00 ± 0.58) and the dilution of 10 mg ml-1 had the most suitable performance. The enzymatic hydrolysis of the Cerastoderma and Didacta showed the same performance in controlling of S. typhi (13.67 ± 4.37 vs 13.33 ± 1.76) and S. paratyphi (17.00 ± 0.58 vs 15.33 ± 1.45). However, the enzymatic hydrolysis of Cerastoderma showed better effect than that of Didacta in controlling S. aureus (18.00 ± 1.15 vs 13.00 ± 2.30), and the 10 and 5 mg ml-1 dilutions were the most appropriate concentrations. It is concluded that Cerastoderma can be used as a resource with potent antibacterial compounds in the preparation of natural antimicrobial agents.</div> Antibacterial activity, Extracts, Cerastoderma, Didacta 1 12 http://ijaah.ir/browse.php?a_code=A-10-176-3&slc_lang=en&sid=1 A Ghorbanalizadeh آسیه قربانعلی زاده eftekar@yahoo.com 10031947532846001804 10031947532846001804 No Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran گروه بیولوژی دریا، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران A Moshfegh اعظم مشفق moshfeghazam@gmail.com 10031947532846001805 10031947532846001805 Yes Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran گروه بیولوژی دریا، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران M Setorki محبوبه سترکی doctor.setorgi@gmail.com 10031947532846001806 10031947532846001806 No Department of Biology, Izeh Branch, Islamic Azad University, Izeh,Iran گروه زیست شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران