fa Treatment stategies with herbal or others Treatment stategies with herbal or others پژوهشي Original research papers <p style="text-align: justify;">The effects of three&nbsp; <a name="OLE_LINK7">anesthetics</a>, <a name="OLE_LINK14">clove oil </a>(<a name="OLE_LINK5">CO</a>) ( 30 mgl^-1), <a name="OLE_LINK13">2-phenoxy ethanol </a>(<a name="OLE_LINK6">2-PE</a>) (0.3 mlL^-1), and MS-222 (100 mgl^-1) were examined on blood enzymes including lactate dehydrogenase (LDH), creatine kinase (CK), alanine aminotransferase (<a name="OLE_LINK8">A</a>LT), aspartate aminotransferase (AST), albumin, glucose, and cortisol levels in the Caspian brown trout. Blood parameters were evaluated in the control (no anesthetics), and three other treatments 10 min and 24 h after the end of anesthesia. Significant increases&nbsp; were seen in blood albumin, ALT, AST, glucose, and cortisol levels in the treatment 24 h following anesthesia by MS-222 compared to the control (p <0.05). Significant increase in blood glucose was also observed in the treatment 10 min after anesthesia with<a name="OLE_LINK10">CO</a>. Post-anesthesia cortisol levels significantly increased in 2-PE treatment after 10 min, in CO treatment after 10 min and 24 h, and in MS-222 treatment following 24 h compared to control. CK activity was significantly decreased only in 2-PE treatment in comparison to control. Except for the treatments of 24 h after anesthesia with MS-222 and 10 min after anesthesia by 2-PE, ALT enzyme activity did not show significant differences in all treatments compared to control (p >0.05). As results, MS-222 is less stressful than both 2-PE and <a name="OLE_LINK11" style="background-color: rgb(255, 255, 255);">CO </a>in short term. The long term stressing effect of 2-PE was detected to be lesser than both MS-222 and CO.&nbsp; So, based on the enzymatic and hormonal responses, MS-222 can be effectively used to reduce severe instantaneous stress such as surgery and spawning.</p> Caspian brown trout, 2-phenoxy ethanol, clove oil, MS-222, Blood enzymes 47 60 http://ijaah.ir/browse.php?a_code=A-10-35-44&slc_lang=fa&sid=1 M Bahrekazemi 10031947532846002339 10031947532846002339 Yes Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran N Yousefi 10031947532846002340 10031947532846002340 No Department of Fisheries, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran