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Published articles: 123
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M.j Zorriehzahra, S Kakoolaki, M Mehrabi, A Sepahdari, M Ghasemi, M Yarmohammadi, M Ghiasi,
Volume 5, Issue 1 (5-2019)
Abstract

In the year 2017, Iran had fisheries production amounted to 1 million tons, of which the total aquaculture was about 300 thousand tons that 160 thousand tons of aquaculture was regarding Oncorhynchus mykiss. Increase of fish stocking density in the pond results in escalation of the number of nutrients, the stresses and incidence of diseases. Among these diseases viral diseases could be identified in the field of rearing ponds, such as Infectious hematopoietic necrosis (IHN), Viral Hemorrhagic Septicemia (VHS) and Infectious Pancreatic Necrosis (IPN). Unlike other projects that sample is taken from fish tissues known as a sample, in this study each farm was selected as a research unit. According to the layout of the work based on different fields; the positive or negative result of the presence of the pathogens was recorded.  However, aquatic samples were collected from Mazandaran province and Yasuj sites, located in north and southwest of Iran, respectively. Based on the results of Mazandaran area, it was revealed that the increase of bio-safety in the pre-broodstock farms did not have a significant difference (p> 0.05) compared with the increase of immunity level or serum antibodies. This index was relatively equal in fish of other areas. Contrarily, in the pre-broodstock farms, the lysozyme value as a non-specific immunity index had a significant increase, which indicates that the upgrading of the bio-safety level can reduce stress and shift the energy directly to the production.
 

M Khosravi, P Shohreh, R Khoshbakht,
Volume 5, Issue 2 (11-2019)
Abstract

Viral hemorrhagic septicemia virus (VHSV), Infectious hematopoietic necrosis virus (IHNV), and infectious pancreatic necrosis virus (IPNV) are documented as the most considerable viral pathogens in Rainbow trout (Onchorhynchus mykiss). This study aimed to evaluate the frequency of these pathogens in 65 farms with suspected clinical signs in northern Iran from March 2016 to February 2018. Logistic regression analysis used to assess the effect of several determinant factors on the occurrence of these pathogens. In total, 19 (29.23%) farms were positive by a reverse transcription-polymerase chain reaction (RT-PCR). The frequency of VHSV, IHNV, and IPNV was reported 18.5%, 6.2%, and 4.61%, respectively. The most affected farms (78.95%) used river water. Furthermore, our results revealed that using river water raised the chances of viral disease by 5 times (OR= 5.02; P= 0.01). Thus, using river water was a risk factor for the occurrence of viral pathogens. From four weight groups (A to D); fishes in groups A (fish < 1 gr) and B (1 to 20 gr) were more affected but not statistically significant (P>0.05). This study has provided insight into the frequency of these targeted viruses. Collectively, establishing routine rapid diagnostic programs and setting up basic educational practices can be valuable to design the prevention and control strategies.

 
S. Kakoolaki, S. A. M. Ebne Al-Torab, A. Ghajari, A. A. Anvar, A. Sepahdari, H. Ahari, H. Hoseinzadeh,
Volume 6, Issue 1 (8-2020)
Abstract

The COVID-19 pandemic is now spreading throughout the world affecting the agricultural activities including fish and shrimp culture sectors. Most of the shrimp producing countries particularly in South-east Asia have been affected due to the lockdown, quarantine roles and regulations ordered by the countries, which was assigned to reduce and control the COVID-19 pandemic spreading in the globe. The current establishment of the restriction and quarantine roles has significantly reduced the domestic and international transportations that can seriously affect the shrimp supply chain in the world. Further, the labor shortage, delay in shrimp harvesting and insufficient supply for the processing of shrimp in the plants are other impacts due to coronavirus outbreaks. The impacts of COVID-19 disease on world shrimp aquaculture can be numerous and is varied depending on the several factors. For instance shrimp culture in some countries such as India and Thailand could be seriously affected by insufficient SPF-shrimp broodstocks, labor shortage, transport restrictions, delayed harvesting (results in soft-shell shrimp) and uncertainty in shrimp trade whereas in other regions including Indonesia and Vietnam the Coronavirus-side effects on shrimp industry are relatively low. This review addressed the socio-economic impacts due to COVID-19 on the shrimp aquaculture sector in 2020.

M Hafezieh, S Kakoolaki, M Ghasemi, R Kazempoor, A Hemati,
Volume 8, Issue 1 (8-2022)
Abstract

The objectives to this research were to determine the haematological changes in Oryctolagus cuniculus as an animal model in response to killed hemorrhagic septicemia virus (VHSV) virulent to Oncorhynchus mykiss. This study composed of 5 groups; Treatment 1 (T1) with 1cc virus, treatment 2 (T2) with 0.5cc virus and 0.5cc adjuvant, treatment 3 (T3) with 0.75cc virus and 0.25cc adjuvant, treatment 4 (T4) with 0.25cc virus and 0.75cc adjuvant, and a control for a period of 4 months, once a month IM injection in 5 group of three New Zealand rabbits with health certification prepared by Razi Vaccine and Serum Research Institute. After 15 days, blood parameters, hematocrit (HCT), hemoglobin (HGB), mean hemoglobin concentration in red blood cells (MCH), red blood cells (RBC), average concentration of red blood cell (MCHC), white blood cells (WBC), neutrophils (SEG), lymphocytes (Lyn), eosinophil (Eos), aspirate transaminase (AST) and alanine transaminase (ALT).The results  showed that ALT, AST, HCT, HGB and WBC concentrations of all rabbits significantly were difference (p < 0.05). T2 (0.5cc virus and 0.5cc adjuvant) and T3 (0.75cc virus and 0.25cc adjuvant) showed higher ALT, AST, HCT and WBCs among other treatments in all 4 blood samplings and among these treatments, T2 with lower levels of ALT and AST (indicating less pressure on the liver) and higher levels of WBCs which can lead to greater amount of neutralizing polyclonal antibodies indicated better results.

 


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