:: Volume 8, Issue 1 (2022) ::
Sustainable Aquaculture. Health. Management. J. 2022, 8(1): 61-77 Back to browse issues page
Research Article: Blood and enzyme factors changes in New Zealand rabbit (Oryctolagus cuniculus) as an animal model in response to killed hemorrhagic septicemia virus (VHSv) virulent to Oncorhynchus mykiss
M Hafezieh , S Kakoolaki * , M Ghasemi , R Kazempoor , A Hemati
Abstract:   (2404 Views)
The objectives to this research were to determine the haematological changes in Oryctolagus cuniculus as an animal model in response to killed hemorrhagic septicemia virus (VHSV) virulent to Oncorhynchus mykiss. This study composed of 5 groups; Treatment 1 (T1) with 1cc virus, treatment 2 (T2) with 0.5cc virus and 0.5cc adjuvant, treatment 3 (T3) with 0.75cc virus and 0.25cc adjuvant, treatment 4 (T4) with 0.25cc virus and 0.75cc adjuvant, and a control for a period of 4 months, once a month IM injection in 5 group of three New Zealand rabbits with health certification prepared by Razi Vaccine and Serum Research Institute. After 15 days, blood parameters, hematocrit (HCT), hemoglobin (HGB), mean hemoglobin concentration in red blood cells (MCH), red blood cells (RBC), average concentration of red blood cell (MCHC), white blood cells (WBC), neutrophils (SEG), lymphocytes (Lyn), eosinophil (Eos), aspirate transaminase (AST) and alanine transaminase (ALT).The results  showed that ALT, AST, HCT, HGB and WBC concentrations of all rabbits significantly were difference (p < 0.05). T2 (0.5cc virus and 0.5cc adjuvant) and T3 (0.75cc virus and 0.25cc adjuvant) showed higher ALT, AST, HCT and WBCs among other treatments in all 4 blood samplings and among these treatments, T2 with lower levels of ALT and AST (indicating less pressure on the liver) and higher levels of WBCs which can lead to greater amount of neutralizing polyclonal antibodies indicated better results.

Keywords: Viral Hemorrhagic Septicemia virus, Rabbit, Hematology, Enzymes
Full-Text [PDF 473 kb]   (205 Downloads)    
Type of Study: Original research papers | Subject: Viral Diseases and viology
Received: 2022/04/25 | Accepted: 2022/08/19 | Published: 2022/08/23
1. Ahne, W., 1982. Vergleichende Untersuchung iiber die Stabilitiit von vier fischpatogener Viren (VHSV. PFR, SVCV, IPNV). Zentralblatt fur Veterinarmedizin, 12, 121-134. [DOI:10.1111/j.1439-0450.1982.tb01248.x]
2. Bokai, S., Absalan Fard, K., Falah Mehrabadi, M., Ebrahimzadeh Mousavi, H., Qajari, A. and Shahbazi, N., 2016. Investigating Viral Hemorrhagic Septicemia outbreaks and factors affecting it in trout farms in the country. Iranian Journal of Epidemiology, 13(3), 253-261.
3. Bradford, M.M., 1976. A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-252. [DOI:10.1016/0003-2697(76)90527-3] [PMID]
4. Dale, O.B., Ørpetveit, I., Lyngstad, T.M., Kahns, S., Skall, H.F., Olesen, N.J. and Dannevig, B.H., 2009. Outbreak of viral hemorrhagic septicemia virus (VHS) in seawater-farmed rainbow trout in Norway caused by VHS virus genotype III. Disease Aquatic Organisms, 85, 93-103. [DOI:10.3354/dao02065] [PMID]
5. Dragoo, J., Braun, H., Durham, J., Ridley, B., Odegaard, J., Luong, R. and Arnoscky, S., 2012. Comparison of the Acute Inflammatory Response of Two Commercial Platelet-Rich Plasma Systems in Healthy Rabbit Tendons. Comparative Study. The American Journal of Sports Medicine, 40(6), 1274-81. [DOI:10.1177/0363546512442334] [PMID]
6. Einer-Jensen, K., Ahrens, P. and Lorenzen, N., 2005. Parallel phylogenetic analyses using the N, G or NV gene from a fixed group of VHSV isolates reveal the same overall genetic typing. Disease Aquatic Organisms, 67, 39-45. [DOI:10.3354/dao067039] [PMID]
7. Einer-Jensen, K., Ahrens, P., Forsberg, R. and Lorenzen, N., 2004. Evolution of the fish rhabdovirus viral hemorrhagic septicemia virus. Journal of Genetic Virololgy, 85, 1167-1179. [DOI:10.1099/vir.0.79820-0] [PMID]
8. Elmas, M., Yazar, E., Uney, K., Er (Karabacak), A. and Tras, B., 2008. Pharmacokinetics of enrofloxacin and flunixin meglumine and interactions between both drugs after intravenous co-administration in healthy and endotoxemia rabbits. Veterinary Journal, 177(3), 418-24. [DOI:10.1016/j.tvjl.2007.05.021] [PMID]
9. Elsayed, E., Faisal, M., Thomas, M., Whelan, G., Batts, W. and Winton, J., 2006. Isolation of viral hemorrhagic septicemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St. Clair, Michigan, USA, reveals a new sub lineage of the North American genotype. Journal of Fish Disease, 29, 611-619. [DOI:10.1111/j.1365-2761.2006.00755.x] [PMID]
10. Emmenegger, E., Hoon Moon, C., Hershberger, P. and Kurath, G., 2013. Virulence of viral hemorrhagic septicemia virus (VHSV) genotypes Ia, IVa, IVb, and IVc in five fish species. Diseases of aquatic organisms. Disease of Aquatic Organisms 12, 107(2):99-111. [DOI:10.3354/dao02671] [PMID]
11. Fijan, N., Sulimanovid, D., Bearzotti, M., Muzinid, D., Zwillenberg, L.O., Chilmonczyk, S., Vautherot, J- F. and Kinkelin, P., 1983. Some properties the epithelioma papulosum cyprinid cell line from carp Cyprinus (EPC). Annales Virology, 134(2), 207-220. [DOI:10.1016/S0769-2617(83)80060-4]
12. Gao, G., Lebherz, C., Weiner, D., Grant, R., Calcedo, R., McCullough, B., Bagg, A., Zhang,Y. and Wilson, J., 2016. Erythropoietin gene therapy leads to autoimmune anemia in macaques. http://www.bloodjournal.org/.
13. Haenen, O.L.M. and Davidse, A., 1993. Comparative pathogenicity of two strains of pike fry rhabdovirus and spring viremia of carp virus for young roach common carp, grass carp and rainbow trout. Disease Aquatic Organisms, 15, 87-92. [DOI:10.3354/dao015087]
14. Hafezieh, M., Ghasemi, M., Kakoolak, S., and Kazempour, R., 2021. Investigation of polyclonal IgG antibody production in response to the injection of killed septicemia virus (VHSV) of rainbow trout (Oncorhyncus mykiss) in Oryctolagus cuniculus rabbit in order to produce a rapid diagnosis kit. Azad University, Sciences and research Branch, Tehran, IRAN DVM, Thesis. 145p.
15. Haghighi Khiabanian Asl, A., Bandehpour, M., Sharifnia, Z. and Kazemi, B., 2008. Diagnosis of viral hemorrhagic septicemia (VHS) in Iranian rainbow trout aquaculture by pathology and molecular techniques. Bulletin European Association Fish Pathology, 28, 170-5.
16. Jensen, M. H., 1963. Preparation of fish tissue cultures for virus research. Bulletin Office International des Epizooties, 59, 131-134.
17. Jensen, M. H., 1965. Research on the virus of Egtved disease. Annales New York Academy Sciences, 126, 422-426. [DOI:10.1111/j.1749-6632.1965.tb14292.x] [PMID]
18. Jørgensen, P.E.V., 1980. Egtved virus: the susceptibility of brown trout and rainbow trout to eight virus isolates and the significance of the findings for the VHS control. In: Ahne, W. (Ed.), Fish Diseases: Third COPRAQ Session. Springer-Verlag, Berlin, Heidelberg, New York, pp. 3-7. [DOI:10.1007/978-3-642-67854-7_1]
19. LÓpez-Vazquez, C., Raynard, R.S., Bain, N., Snow, M., Bandin, I. and Dopazo, C.P., 2006. Genotyping of marine viral hemorrhagic septicemia virus isolated from the Flemish Cap by nucleotide sequence analysis and restriction fragment length polymorphism patterns. Diseases of Aquatic organisms, 73, 23-31. [DOI:10.3354/dao073023] [PMID]
20. Lorenzen, E., Crstensen, B. and Olesen, N.J., 1999. Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Diseases of Aquatic organisms, 37, 81-88. [DOI:10.3354/dao037081] [PMID]
21. Melillio, A., 2007. Rabbit clinical pathology. Journal of Exotic Pet Medicine, 16:135-145. [DOI:10.1053/j.jepm.2007.06.002] [PMID] [PMCID]
22. Meyers, T.R. and Winton, J.R., 1995. Viral hemorrhagic septicemia virus in North America. Annales Review. Fish Disease, 5, 3-24. [DOI:10.1016/0959-8030(95)00002-X]
23. Mojabi, A., Safi, Sh. and Heydar Nejad, A., 2019. Hematology and veterinary medicine and laboratory methods. Publisher: Applied Scientific Higher Education Institute of Agricultural Jihad, 250 pages.
24. Mustafaei, A., 2014. Theoretical and practical guide for protein electrophoresis in gel (first edition). Yadavaran (affiliated with Yadavaran International Research and Publishing Company). 184 pages.
25. Nishizawa, T., Yoshimizu, M., Winton, J., Ahne, W. and Kimura, T., 1991. Characterization of structural proteins of hirame rhabdovirus, HRV. Diseases of Aquatic organisms, Vol. 10, 167-172. [DOI:10.3354/dao010167]
26. OIE, World Organization for animal health, 2021. Final report 2021. 88 GS/FR - PARIS.
27. Olesen, N. J., Lorenzen, N., Aarhus, N., Scott, E. and Lapatra, N., 1999. Production of Neutralizing Antisera against Viral Hemorrhagic Septicemia (VHS) Virus by Intravenous Injections of Rabbits Clear Springs Foods, Inc., Buhl, Idaho 83316, USA. Journal of Aquatic Animal Health, 11, 10-16. [DOI:10.1577/1548-8667(1999)0112.0.CO;2]
28. Olesen, N.J. and Vestergard Jørgsen, P.E., 1982. Can and do herons serve as vectors for Egtved virus? Bulletin European Association Fish Pathology, 2, 48.
29. Peters, F. and Neukirch, M., 1986. Transmission of some fish pathogenic viruses by the heron, Ardera cinera. Journal of Fish Disease, 9, 539-544. [DOI:10.1111/j.1365-2761.1986.tb01050.x]
30. Rahbarizadeh, F., Asadi Karam, Gh. and Rahimi Jamnani, F., 2015. Polyclonal and monoclonal antibodies (from production to application). Tarbiat Modares University. 254 p.
31. Rasmussen, C.J., 1965. A biological study of the Egtved disease (InuL). Annales New York Academy Sciences, 126, 427-460. [DOI:10.1111/j.1749-6632.1965.tb14293.x] [PMID]
32. Reed, L.J. and Muench, H., 1938. A Simple method of estimating fifty percent endpoints. Sanders and Sanders, 248 pp.
33. Skall, H.F., Olesen, N.J. and Mellergaard, S., 2005. Prevalence of viral hemorrhagic septicemia virus in Danish marine fishes and its occurrence in new host species. Disease Aquatic Organisms, 66, 145-151. [DOI:10.3354/dao066145] [PMID]
34. Smail, D.A., 1999. Viral hemorrhagic septicemia. In: Fish Diseases and Disorders, Volume 3: Viral, Bacterial and Fungal infections, Woo P.T.K. and Bruno D.W., eds, 123-147.
35. Snow, M., Bain, N., Black, J., Taupin, V., Cunnigham, C.O., King, J.A., Skall, H.F. and Raynard, R.S., 2004. Genetic population structure of marine viral hemorrhagic septicemia virus (VHSV). Disease Aquatic Organisms, 61, 11-21. [DOI:10.3354/dao061011] [PMID]
36. Snow, M., Cunningham, C.O., Melvin, W.T. and Kurath, G., 1999. Analysis of the nucleoprotein gene identifies distinct lineages of viral hemorrhagic septicemia virus within the European marine environment. Virus Research, 63, 35-44. [DOI:10.1016/S0168-1702(99)00056-8] [PMID]
37. Tordo, N., Benmansour, A., Calisher, C., Dietzgen, RG., Fang, RX., Jackson, AO., Kurath, G., Nadin-Davis, S., Tesh, RB. and Walker, PJ., 2005. Family Rhabdoviridae. In: Fauquet CM. Mayo, MA, Maniloff, J., Desselberger, U., Ball, LA., (Eds.), Virus Taxonomy: Eight Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, 623-533.
38. Walker, P.J., Bnmansour, A., Dietzgen, R. and et.al., 2000. Family Rhabdoviridae. In: Virus Taxonomy classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses, Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L. et al., eds. 563-583.
39. Wolf, K., 1988. Viral hemorrhagic septicemia. In: Fish Viruses and Fish Viral Diseases. Cornell University Press, Ithaca, New York, USA, 217-249.

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