:: Volume 7, Issue 1 (2021) ::
Sustainable Aquaculture. Health. Management. J. 2021, 7(1): 21-31 Back to browse issues page
Research Article: Effects of pH-induced technique on flocculation efficiency and fatty acids profile of marine microalgae, Thalassiosira sp
M Hobbi , P Akbary * , Z Aminikhoei
Abstract:   (2109 Views)
Microalgae contain many valuable biological compounds, so their commercial production is of great importance. One of the important challenges in microalga production is their high cost associated with their harvesting processes. Therefore, finding efficient and cost-effective technologies for algae biomass separation is essential for achieving an operational process. The study was conducted to find the influence of pH variation from 4 to 11 on harvesting efficiency and fatty acids component of marine microalgae Thalassiosira sp under laboratory condition. The NaOH and HCl were added to adjust pH value in each treatment. Data was presented as mean ± SD. Comparison between treatments was performed using one-way analysis of variance ANOVA. The results showed that with increasing the pH up to 10, flocculation processes (92%) and concentration factor (20) increased significantly. While, lowering the pH from 8.2 to 4 was not effective in isolating algal biomass. In the next stage, the biomass fatty acids content was measured by both alkaline pH induction method and the centrifuge method. Analyzes showed that the percentage of lipid and saturated and unsaturated fatty acids in biomass collected by alkaline pH induction technique was lower than the centrifugation method. In general, the results of the present study showed that clotting in alkaline pH up to 10 can be suggested as a suitable method for harvesting of Thalassiosira sp microalgae, and if the production of polyunsaturated acids is considered, of consuming high amounts of sodium hydroxide should be avoided.
Keywords: Microalgae, Thalassiosira sp, pH-induced, Harvesting efficiency, Fatty acids
Full-Text [PDF 524 kb]   (830 Downloads)    
Type of Study: Original research papers | Subject: Biotechnology
Received: 2021/06/10 | Accepted: 2021/08/11 | Published: 2021/08/14
References
1. Barros, , A. I., Gonçalves, A. L., Simões, M. and Pires, J. C. 2015., Harvesting techniques applied to microalgae: a review. Renewable and Sustainable Energy Reviews, 41, 1489-1500. [DOI:10.1016/j.rser.2014.09.037]
2. Becker, W., 2004. 18 Microalgae in Human and Animal Nutrition. Handbook of microalgal culture: biotechnology and applied phycology, 312- 351. [DOI:10.1002/9780470995280.ch18]
3. Besson, A. and Guiraud, P., 2013. High-pH-induced flocculation-flotation of the hypersaline microalga Dunaliella salina. Bioresource technology, 147, 464-470. [DOI:10.1016/j.biortech.2013.08.053] [PMID]
4. Borowitzka,, M. A. and Borowitzka, L. J., 1988. Micro-algal biotechnology, Cambridge University Press.
5. Garcia, N., Lŏpez-ELias, J. A., Miranda, A., Martinez-Pprrchas, M., Hureta, N. and Garcia, A., 2012. Effect of salinity on growth and chemical composition of the diatom Thalassiosira weissflogii at three culture phases. Latin American Journal of Aquatic Research, 40, 435-440. [DOI:10.3856/vol40-issue2-fulltext-18]
6. Guillard, R. R., 1975. Culture of phytoplankton for feeding marine invertebrates. Culture of marine invertebrate animals. Springer. [DOI:10.1007/978-1-4615-8714-9_3]
7. Horiuchi, J.-I., Ohba, I., Tada, K., Kobayashi, M., Kanno, T. and Kishimoto, M., 2003. Effective cell harvesting of the halotolerant microalga Dunaliella tertiolecta with pH control. Journal of bioscience and bioengineering, 95, 412-415. [DOI:10.1016/S1389-1723(03)80078-6]
8. Iso, U., 2011. 12966-2 Animal and Vegetables Fat and Oils. Gas Chromatography of Fatty Acid Methyl Esters. Part 2: Preparation of Methyl Esters of Fatty Acids. International Organization for Standardization: Geneva, Switzerland.
9. KRISHNAKUMAR, S., BAI, V. D. M. & RAJAN, R. A. 2013. Evaluation of bioactive metabolites from halophilic microalgae Dunaliella salina by GC-MS analysis. Int. J. Pharm. Pharm. Sci, 5, 296-303.
10. Kwon, H., Lu, M., Lee, E. Y. and Lee, J., 2014. Harvesting of microalgae using flocculation combined with dissolved air flotation. Biotechnology and bioprocess engineering, 19, 143-149. [DOI:10.1007/s12257-013-0433-y]
11. Lang, Y., Del Monte, F., Rodriguez, B. J., Dockery, P., Finn, D. P. and Pandit, A., 2013. Integration of TiO 2 into the diatom Thalassiosira weissflogii during frustule synthesis. Scientific reports, 3, 3205-3216. [DOI:10.1038/srep03205] [PMID] [PMCID]
12. Liu, J., Zhu, Y., Tao, Y., Zhang, Y., Li, A., Li, T., Sang, M. and Zhang, C., 2013. Freshwater microalgae harvested via flocculation induced by pH decrease. Biotechnology for biofuels, 6, 98-109. [DOI:10.1186/1754-6834-6-98] [PMID] [PMCID]
13. Maji, G., Choudhury, S., Hamid, S., Prashanth, R. and Sibi, G., 2018. Microalgae harvesting via flocculation: impact of pH, algae species and biomass concentration. Methods of Microbiology and Molecular Biology, 1, 1-5.
14. Milledge, J. J. and Heaven, S., 2013. A review of the harvesting of micro-algae for biofuel production. Reviews in Environmental Science and Bio/Technology, 12, 165-178. [DOI:10.1007/s11157-012-9301-z]
15. Mixson, S. M., Stikeleather, L. F., Simmons, O. D., Wilson, C. W. and Burkholder, J. M., 2014. pH-induced flocculation, indirect electrocoagulation, and hollow fiber filtration techniques for harvesting the saltwater microalga Dunaliella. Journal of applied phycology, 26, 1701-1709. [DOI:10.1007/s10811-013-0232-z]
16. Pėrez, L., Salgueiro, J. L., Maceiras, R., Cancela, Á. and Sānchez, Á., 2017. An effective method for harvesting of marine microalgae: pH induced flocculation. Biomass and Bioenergy, 97, 20-26. [DOI:10.1016/j.biombioe.2016.12.010]
17. Sales, R. and Abreu, P. C., 2015. Use of natural pH variation to increase the flocculation of the marine microalgae Nannochloropsis oculata. Applied biochemistry and biotechnology, 175, 2012-2019. [DOI:10.1007/s12010-014-1412-2] [PMID]
18. Show, K.Y., Lee, D. J. and Chang, J. S., 2013. Algal biomass dehydration. Bioresource technology, 135, 720-729. [DOI:10.1016/j.biortech.2012.08.021] [PMID]
19. Spilling, K., Seppālā, J. and Tamminen, T., 2011. Inducing autoflocculation in the diatom Phaeodactylum tricornutum through CO 2 regulation. Journal of applied phycology, 23, 959-966. [DOI:10.1007/s10811-010-9616-5]
20. Uduman, N., Qi, Y., Danqah, M. K., Forde, G. M. and Hoadley, A., 2010. Dewatering of microalgal cultures: a major bottleneck to algae-based fuels. Journal of renewable and sustainable energy, 2, 012701. [DOI:10.1063/1.3294480]
21. Wan, C., Alam, M. A., Zhao, X. Q., Zhang, X. Y., Guo, S.-L., Ho, S. H., Chang, J.-S. and Bai, F. W., 2015. Current progress and future prospect of microalgal biomass harvest using various flocculation technologies. Bioresource technology, 184, 251-257. [DOI:10.1016/j.biortech.2014.11.081] [PMID]
22. Wu, Z., Zhu, Y., Huang, W., Zhang, C., Li, T., Zhang, Y. and Li, A., 2012. Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium. Bioresource technology, 110, 496-502. [DOI:10.1016/j.biortech.2012.01.101] [PMID]
23. Yang, F., Xiang, W., Fan, J., Wu, H., Li, T. and Long, L., 2016. High pH-induced flocculation of marine Chlorella sp. for biofuel production. Journal of applied phycology, 28, 747-756. [DOI:10.1007/s10811-015-0576-7]



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