:: Volume 3, Issue 1 (2017) ::
Sustainable Aquaculture. Health. Management. J. 2017, 3(1): 16-25 Back to browse issues page
The effect of dietary propolis and pollen extracts on growth performance and haematological responses of rainbow trout (Onchorhynchus mykiss)
N Choobkar * , S Kakoolaki , F Mohammadi , M Rezaeimanesh
Abstract:   (6173 Views)

The aim of this study was to determine the effect of propolis and pollen extract on biochemical and haematological parameters of Onchorhynchus  mykiss. One hundred and eighty healthy fish, O. mykiss (mean weight 20.57±4.48 g) were used for growth performance and haematological assays. They were randomly selected, and distributed among fifteen 600-L fiberglass tanks as three treatments (1% Pollen, 1% Proplils, 0.5% Pollen + 0.5% Proplils as groups 1-3, respectively), negative control as group 4 in triplicates at density of 30 fish each. After 8 weeks of the experiment, which the fish fed supplemented diets, final weight of all treatments showed no significant difference (p> 0.05) as well as WG and DGI. Definitely, all the treatments of the mentioned criteria showed a significant increase (p< 0.05) compared to control group. On the other hand, FCR showed a significance difference (p= 0.00) among the treatments and control so that its value for the group 3 was the minimum one (1.84±0.05) and followed by group 2 (2.36±0.03). Some criteria containing Hb, RBC, Hct and WBC showed a remarkable increase (p< 0.05) in group 3 compared to other groups while MCV and MCH was significantly lower in group 3 (p< 0.05).
Keywords: herbal medicine, Pollen, Propolis, Onchorhynchus mykiss, haematology
Full-Text [PDF 266 kb]   (2175 Downloads)    
Type of Study: Original research papers | Subject: Treatment stategies with herbal or others
Received: 2017/01/20 | Accepted: 2017/03/30 | Published: 2017/04/7
References
1. Abd-El-Rhman, A.M. (2009) Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticus. Fish & shellfish immunology 27(3), 454-459. [DOI:10.1016/j.fsi.2009.06.015]
2. Ardó, L., Yin, G., Xu, P., Váradi, L., Szigeti, G., Jeney, Z. & Jeney, G. (2008) Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture 275(1), 26-33. [DOI:10.1016/j.aquaculture.2007.12.022]
3. Attia, Y., Al‐Hanoun, A., Tag El‐Din, A., Bovera, F. & Shewika, Y. (2011) Effect of bee pollen levels on productive, reproductive and blood traits of NZW rabbits. Journal of animal physiology and animal nutrition 95(3), 294-303. [DOI:10.1111/j.1439-0396.2010.01054.x]
4. Dacie, J.V. & Lewis, S.M. (2001) Miscellaneous tests. Churchill Livingstone, Dacie and Lewis practical haematology.
5. Dinakaran Michael, R., Srinivas, S., Sailendri, K. & Muthukkaruppan, V. (1994) A rapid method for repetitive bleeding in fish. Indian Journal of Experimental Biology 32, 838-838.
6. El-Asely, A.M., Abbass, A.A. & Austin, B. (2014) Honey bee pollen improves growth, immunity and protection of Nile tilapia (Oreochromis niloticus) against infection with Aeromonas hydrophila. Fish & shellfish immunology 40(2), 500-506. [DOI:10.1016/j.fsi.2014.07.017]
7. FAO (2014) The state of the world fisheries and aquaculture. Rome, FAO.
8. FAO (2016) The state of world Fisheries and Aquaculture. Rome.
9. Ferreira, D., Rocha, H.C., Kreutz, L.C., Loro, V.L., Marqueze, A., Koakoski, G., da Rosa, J.G.S., Gusso, D., Oliveira, T.A. & de Abreu, M.S.(2013) Bee products prevent agrichemical-induced oxidative damage in fish. PloS one 8(10), e74499. [DOI:10.1371/journal.pone.0074499]
10. Grant, K.R. (2015) Fish hematology and associated disorders. Veterinary Clinics of North America: Exotic Animal Practice 18(1), 83-103. [DOI:10.1016/j.cvex.2014.09.007]
11. Harikrishnan, R., Balasundaram, C. & Heo, M.-S. (2012) Effect of Inonotus obliquus enriched diet on hematology, immune response, and disease protection in kelp grouper, Epinephelus bruneus against Vibrio harveyi. Aquaculture 344, 48-53. [DOI:10.1016/j.aquaculture.2012.03.010]
12. Harikrishnan, R., Kim, J.-S., Kim, M.-C., Balasundaram, C. & Heo, M.-S. (2011) Styrax japonica supplementation diet enhances the innate immune response in Epinephelus bruneus against bacterial and protozoan infections. Experimental parasitology 129(3), 260-265. [DOI:10.1016/j.exppara.2011.07.017]
13. Kakoolaki, S., Talas, Z.S., Cakir, O., Ciftci, O. & Ozdemir, I. (2013) Role of Propolis on Oxidative Stress in Fish Brain. Basic and Clinical Neuroscience 4(2), 153-158.
14. Moreira, L., Dias, L.G., Pereira, J.A. & Estevinho, L. (2008) Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food and Chemical toxicology 46(11), 3482-3485. [DOI:10.1016/j.fct.2008.08.025]
15. Oskoii, S.B., Kohyani, A.T., Parseh, A., Salati, A.P. & Sadeghi, E. (2012) Effects of dietary administration of Echinacea purpurea on growth indices and biochemical and hematological indices in rainbow trout (Oncorhynchus mykiss) fingerlings. Fish physiology and biochemistry 38(4), 1029-1034. [DOI:10.1007/s10695-011-9587-8]
16. Řehulka, J. (2002) Aeromonas causes severe skin lesions in rainbow trout (Oncorhynchus mykiss): clinical pathology, haematology, and biochemistry. Acta Veterinaria Brno 71(3), 351-360. [DOI:10.2754/avb200271030351]
17. Sapkota, A., Sapkota, A.R., Kucharski, M., Burke, J., McKenzie, S., Walker, P. & Lawrence, R. (2008) Aquaculture practices and potential human health risks: current knowledge and future priorities. Environment international 34(8), 1215-1226. [DOI:10.1016/j.envint.2008.04.009]
18. Selamoglu Talas, Z. & Gulhan, M.F. (2009) Effects of various propolis concentrations on biochemical and hematological parameters of rainbow trout (Oncorhynchus mykiss). Ecotoxicology and Environmental Safety 72(7), 1994-1998. [DOI:10.1016/j.ecoenv.2009.04.011]
19. Selamoglu Talas, Z., Pinar Dundar, S., Fuat Gulhan, M., Orun, I. & Kakoolaki, S. (2012) Effects of propolis on some blood parameters and enzymes in carp exposed to arsenic. Iranian Journal of Fisheries Sciences 11(2), 405-414.
20. Sforcin, J., Fernandes, A., Lopes, C., Bankova, V. & Funari, S. (2000) Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology 73(1), 243-249. [DOI:10.1016/S0378-8741(00)00320-2]
21. Soltni M., Kakoolaki S., Kisami M. (2000) Isolation and identification of dominant Vibrio species in farmed prawn of Heleh station, Bushehr. Journal of the Faculty of Veterinary Medicine, University of Tehran. 55(2), 29-32.
22. Soltani, M., Pirali, E., Rasoli, A., Kakoolaki, S. & Shams, G. (2014) Antibiotic residuals in some farmed rainbow trout(Oncorhynchusmykiss) of market size in Iran. Iranian Journal of Aquatic Animal Health 1(1), 71-77.
23. Wang, J., Li, S., Wang, Q., Xin, B. & Wang, H. (2007) Trophic effect of bee pollen on small intestine in broiler chickens. Journal of Medicinal Food 10(2), 276-280. [DOI:10.1089/jmf.2006.215]
24. Xu, X., Sun, L., Dong, J. & Zhang, H. (2009) Breaking the cells of rape bee pollen and consecutive extraction of functional oil with supercritical carbon dioxide. Innovative food science & emerging technologies 10(1), 42-46. [DOI:10.1016/j.ifset.2008.08.004]

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