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:: Volume 4, Issue 1 (4-2018) ::
I. j. Aqua. Anim. Health 2018, 4(1): 109-123 Back to browse issues page
Applying new formulated herbal anesthetic comparing to tricaine methanesulfonate (MS-222) in beluga (Huso huso)
H Gholipour Kanani , P Hooshmand, M Harsij, E Gholamalipour Alamdari
Abstract:   (495 Views)

Applying anaesthetic agent is recommended in aquaculture during fish handling specially tagging. However, it should be noted that anesthesia itself is likely to induce stressful condition due to its improper and high concentration. By means of nanoemulsions, drug delivery improves which in turn contributes to fewer amounts of pharmaceutical agents’ usage. In this research, nanoemulsions of clove oil (NCL) and lemonbeebrush (NLB) were used to develop the anesthetic efficacy and reduce the concentration needed for anesthesia in Huso huso (mean weight: 1115 ± 242.25 g) as an endangered species. Based on the results, anesthesia induction time (s) of stage 3 & 4 was significantly decreased in NCL and NLB when compared to MS-222 (p<0.05). NCL treated beluga were induced stage 5 anesthesia much quicker than NLB and MS-222. In contrary, recovery time did not show significant difference among treatments (p>0.05). Among measured biochemical factors, lactate had the highest value in NCL group during anesthesia (p<0.05). In contrast, lactate level had highest level in NLB, 24h post anesthesia (p<0.05). NLB and NCL produced higher non-specific immune (Lysozyme and ACH50) enhancements at 0 and 24h post anesthesia. Furthermore, results showed that MS-222 induced increased serum cortisol levels (p<0.05). Cortisol and glucose were not depressed by NLB and NCL, suggesting that these anesthetics are safer anesthetic for Huso huso as it does not cause immune depression in anesthetized fish.

Keywords: Aloysia triphylla, Eugenia caryophyllata, Innate immunity, Anesthesia, Great sturgeon
Full-Text [PDF 387 kb]   (92 Downloads)    
Type of Study: Research | Subject: Aquaculture and Health management
Received: 2017/12/13 | Accepted: 2018/04/15 | Published: 2018/04/15
1. Azad I.S., Al-Yaqout A.& Al-Roumi M. (2014) Antibacterial and immunity enhancement properties of anaesthetic doses of thyme (Thymus vulgaris) oil and three other anaesthetics in Sparidentaxhasta and Acanthopagrus latus. Journal of King Saud University 26,101-106. [DOI:10.1016/j.jksus.2013.12.003]
2. Bagheri T. & Imanpour M.R. (2011) The Efficacy, Physiological Responses and Hematology of Persian Sturgeon, Acipenser persicus, to Clove Oil as an Anesthetic Agent. Turkish Journal of Fisheries and Aquatic Sciences 11, 477-483. [DOI:10.4194/1303-2712-v11_3_20]
3. Boyer S.E, White J.S., Stier A.C.& Osenberg C.W. (2009) Effects of the fish anesthetic, clove oil (eugenol), on coral health and growth. Journal of Experimental Marine Biology and Ecology 369, 53–57. [DOI:10.1016/j.jembe.2008.10.020]
4. Carnat A., Carnat A.P., Fraisse D. & Lamaison J.L. (1999) The aromatic and polyphenolic composition of lemon verbena tea. Fitoterapia 70 (1), 44–49. [DOI:10.1016/S0367-326X(98)00016-1]
5. Cataldi E., Marco P.D., Mandich A. & Cataudella S. (1998) Serum parameters of Adriatic sturgeon Acipenser naccarii (Pisces: Acipenseriformes): effect of temperature and stress. Comparative biochemistry. Physiology A121, 351–354. [DOI:10.1016/S1095-6433(98)10134-4]
6. Chaney A.L. & Merbach E.P. (1962) Modified reagents for determination of urea and ammonia. Clinical Chemistry 8, 130-132.
7. Czesny S., Rinchard J., Garcia M.A. & Dabrowski K.D. (2003) The effect of fasting, prolonged swimming, and predator presence on energy utilization and stress in juvenile walleye (Stizostedion vitreum). Physiological behavior 79, 597–603. [DOI:10.1016/S0031-9384(03)00124-0]
8. Ebrahimi P. (2014) Topical Quercetin Nanoemulsions: Optimization of preparation using chemometric approaches. Pharmaceutical Chemistry Journal 48(6) 402–407. [DOI:10.1007/s11094-014-1120-9]
9. Feng G., Liu J., Zhuang P., Zhang L.& Duan M. (2011) Anesthesia and recovery with clove oil in juvenile Siberian sturgeon (Acipenser baerii). Bioinformatics and Biomedical Engineering, (ICBBE) 5th International Conference, 10-12 May, Wuhan, China: 1-4. [DOI:10.1109/icbbe.2011.5781404]
10. Frisch A. J., Ulstrup K.E., Hobbs J.P.A. (2007) The effects of clove oil on coral: An experimental evaluation using Pocillopora damicornis. Journal of experimental marine biology and ecology 345, 101–109. [DOI:10.1016/j.jembe.2007.02.004]
11. Gressler L.T., Riffel A.P.K., Parodi T.V., Saccol E.M.H., Koakoski G., Costa S.T., Pavanato M.A., Heinzmann B.M., Caron B., Schmidt D., Liesuy S.F., Barcellos L.J.G. & Baldisserotto B. (2014) Silver catfish Rhamdia quelen immersion anaesthesia with essential oil of Aloysia triphylla (L'Herit) Britton or tricaine methanesulfonate: effect on stress response and antioxidant status. Aquaculture research 45, 1061–1072. [DOI:10.1111/are.12043]
12. Grutter A.S. & Pankhurst N.W. (2000) The effects of capture, handling, con¢nement and ectoparasite load on plasma levels of cortisol, glucose and lactate in the coral reef fish Hemigymnus melapterus. Journal of Fish Biology 57(2), 391- 401. [DOI:10.1111/j.1095-8649.2000.tb02179.x]
13. Guglielmini G.(2008). Nanostructured novel carrier for topical application. Clinical Dermatology 26, 341-346. [DOI:10.1016/j.clindermatol.2008.05.004]
14. Hoseini S.M.& Ghelichpour M. (2012) Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.). Fish Physiology and Biochemistry 38(2) 493-8. [DOI:10.1007/s10695-011-9529-5]
15. Hoseini S.M., Hosseini S.A. & Jafar Nodeh A. (2011) Serum biochemical characteristics of Beluga, Huso huso (L.), in response to blood sampling after clove powder solution exposure. Fish Physiology and Biochemistry 37(3), 567–572. [DOI:10.1007/s10695-010-9458-8]
16. Imanpoor M.R., Bagheri T., Hedayeti S.A.A. (2010) The Anesthetic Effects of Clove Essence in Persian Sturgeon, Acipenser persicus. World Journal of Fish Marine Science 2(1), 29-36.
17. Iwama G.K., McGeer J.C. & Pawluk M.P. (1989) The effect of anesthetics in five fishes on acid-base balance, hematocrit, blood gases, cortisol, and adrenaline in rainbow trout. Canadian Journal of Zoology 67, 2065–2073. [DOI:10.1139/z89-294]
18. Johnson, J.L., Trushenski, J.T. & Bowker, J.D. (2016) Induction, recovery, and hematological responses of Pallid Sturgeon to chemical and electrical sedation. North American Journal of Fisheries Management 36(3):568–575. [DOI:10.1080/02755947.2016.1141121]
19. Keene J.L., Noakes D.L.G., Moccia R.D. & Soto C.G. (1998) The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research 29, 89-101. [DOI:10.1111/j.1365-2109.1998.tb01113.x]
20. Marking L. L. & Meyer F.P. (1985) Are better anaesthetics needed in fisheries?. Fisheries 10(6), 2-5. https://doi.org/10.1577/1548-8446(1985)010<0002:ABANIF>2.0.CO;2 [DOI:10.1577/1548-8446(1985)0102.0.CO;2]
21. Matsuyama H., Tanaka K., Nakao M. & Yano T. (1988) Characterization of the alternative complement pathway of carp. Developmental Comparative Immunology 12, 403–408. [DOI:10.1016/0145-305X(88)90015-8]
22. Miller E.C., Swanson A.B., Phillips D.H., Flethcer T.L., Liem A., Miller J.A. (1989) Structure-activity studies of the carcino-genicities in the mouse and rat of some naturally occurring and synthetic alkenylbenzene derivatives related to safrole and estragole. Cancer Research 43,11-24.
23. Mohammadi Arani M., Shahbaz S. & Safari O. (2013) A study on the anesthetization of persian sturgeon (Acipenser persicus) fingerlings in three weight groups; using clove (Eugenia caryophyllata) oil. International Research Journal of Applied & Basic Science 5(11) 1454-1460.
24. Mohammadizarejabad A., Bastami K.D., Sudagar M. & Pourali Motlagh S. (2009) Hematology of great sturgeon (Huso huso Linnaeus, 1758) juvenile exposed to clove powder as an anesthetic. Comparative Clinical Pathology 19(5) 465–468. [DOI:10.1007/s00580-009-0886-8]
25. Mulochau T. & Durville P. (2004) Effects of a clove oil-ethanol solution on the coral Pocillopora verrucosa. Revue d'Ecologie la Terre et la Vie 59, 425–432.
26. Needham D.G. (1998) Anaesthesia and surgery. Timber press. USA, 586 p.
27. Ortuno J., Esteban M.A., Meseguer J. (2002) Effects of four anaesthetics on the innate immune response of gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology 12(1), 49–59. [DOI:10.1006/fsim.2001.0353]
28. Park J.W., Park E.S., Chi S.C., Kil H.Y. & Lee K.H. (2003) The effect of lidocaine on the globule size distribution of propofol emulsions. Anesthesia & Analgesia 97(3), 769–71. [DOI:10.1213/01.ANE.0000074797.70349.CA]
29. Parodi T.V., Cunha M.A., Heldwein C.G., Souza D.M., Martins A.C., Garcia L.O., Wasielesky J.W., Monserrat J.M., Schmidt D., Caron BO, Heinzmann B & Baldisserotto B (2012) The anesthetic efficacy of eugenol and the essential oils of Lippia alba and Aloysia triphylla in post-larvae and subadults of Litopenaeus vannamei (Crustacea, Penaeidae). Comparative Biochemical Physiology Part C: Toxicology & Pharmacology 155(3), 462–468.
30. Pirhonen J. & Shreck C.B. (2003) Effects of anaesthesia with MS-222, clove oil and CO 2 on feed intake and plasma cortisol in steelhead trout (Oncorhynchus mykiss). Aquaculture 220, 507-514. [DOI:10.1016/S0044-8486(02)00624-5]
31. Ross L.G. & Ross B. (2008) Anaesthetic and sedative techniques for aquatic animals, 3rd edition. Blackwell Scientific Publications, Oxford, UK. [DOI:10.1002/9781444302264]
32. Rotllant J., Balm P.H., Perez-Sanchez J., Wendelaar-Bonga S.E. & Tort L. (2001) Pituitary and interregnal function in gilthead sea bream (Sparus aurata L., Teleostei) after handling and confinement stress. General Comparative Endocrinology 121(3), 333–342. [DOI:10.1006/gcen.2001.7604]
33. Sahoo P.K., Meher P.K., Mahapatra K.D., Saha J.N., Jana R.K. & Reddy P.V.G.K. (2004) Immune responses in fullsib families of Indian major carp,Labeo rohita, exhibiting resistance to Aeromonas hydrophila infection. Aquaculture 238, 115-125. [DOI:10.1016/j.aquaculture.2004.06.008]
34. Shaluei F., Hedayati A., Jahanbakhshi A. & Baghfalaki M. (2012) Physiological responses of great sturgeon (Huso huso) to different concentrations of 2-phenoxyethanol as an anesthetic. Fish Physiology & Biochemistry 38(6), 1627–1634. [DOI:10.1007/s10695-012-9659-4]
35. Shi X., Li D., Zhuang P., Nie F. & Long L. (2006) Comparative blood biochemistry of Amur sturgeon, Acipenserschrenckii, and Chinese sturgeon, Acipenser sinensis. Fish Physiology and Biochemistry 32, 63–66. [DOI:10.1007/s10695-006-7134-9]
36. Skjervold P.O., Fjaera S.O. & Oestby P.B. (1999) Rigor in atlantic salmon as affected by crowding stress prior to chilling before slaughter. Aquaculture 175, 93–101. [DOI:10.1016/S0044-8486(99)00037-X]
37. Soto C.G. & Burhanuddin S. (1995) Clove oil as a fish anaesthetic for measuring length and weight of rabbit fish (Siganus lineatus). Aquaculture 136, 149-152. [DOI:10.1016/0044-8486(95)01051-3]
38. Summerfelt R.C. & Smith L.S. (1990) Anesthesia, surgery, and related techniques. Pages 213 – 263 in C. B. Schreck and P. B. Moyle, editors. Methods for fish biology. American Fisheries Society, Bethesda, Maryland.
39. Thaylise V., Parodi M., Cunha A., Becker A.G., Zeppenfeld C.C., Martins D.I., Koakoski G., Barcellos L.G., Heinzmann B.M. & Baldisserotto B. (2014) Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen. Fish Physiology and Biochemistry 40, 323–334. [DOI:10.1007/s10695-013-9845-z]
40. Velisek J., Svobodova Z. & Piackova V. (2005a) Effects of clove oil anaesthesia on Rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Brunensis 139-146.
41. Velisek J., Svobodova Z., Piackova V., Groch L. & Nepejchalova L. (2005b) Effects of clove oil anaesthesia on Common carp (Cyprinus carpio). Veterinia Medicina-Czech 50(6), 269-275. [DOI:10.17221/5623-VETMED]
42. Wagner G.N., Singer T.D. & McKinley R.S. (2003) The ability of clove oil and MS-222 to minimize handling stress in Rainbow Trout (Oncorhynchus mykiss Walbaum). Aquaculture Research 34, 1139 – 1146. [DOI:10.1046/j.1365-2109.2003.00916.x]
43. Wagner E., Arndt R. & Hilton B. (2002) Physiological stress responses, egg survival and sperm motility for Rainbow Trout broodstock anesthetized with clove oil, tricaine methanesulfonate or carbon dioxide. Aquaculture 211, 353- 366. [DOI:10.1016/S0044-8486(01)00878-X]
44. Weber R.A., Pe´rez-Maceira J.J., Peleteiro J.B., Garcı´a-Martı´n L. & Aldegunde M. (2011) Effects of acute exposure to 2-phen-oxyethanol, clove oil, MS-222, and metomidate on primary and secondary stress responses in Senegalese sole (Solea senegalensis Kaup 1858). Aquaculture 321, 108-112. [DOI:10.1016/j.aquaculture.2011.08.029]
45. Wedemeyer G.A., Barton B.A. & McLeay D.J. (1990) Stress and acclimation. In: Schreck CB, Moyle PB (eds) Methods for fish biology. American Fisheries Society Bethesda 451–489.
46. Wendelaar Bonga S.E. (1997) The stress response in Fish. Physiology Review 77,591–625. [DOI:10.1152/physrev.1997.77.3.591]
47. Woody C.A., Nelson J. & Ramstad K. (2002) Clove oil as an anaesthetic for adult sockeye salmon: field trails. Journal of Fish Biology 60 (2), 340-347. [DOI:10.1111/j.1095-8649.2002.tb00284.x]

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Gholipour Kanani H, Hooshmand P, Harsij M, Gholamalipour Alamdari E. Applying new formulated herbal anesthetic comparing to tricaine methanesulfonate (MS-222) in beluga (Huso huso) . I. j. Aqua. Anim. Health. 2018; 4 (1) :109-123
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